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Determination of vigabatrin by capillary electrophoresis with laser-induced fluorescence detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Aug 25; 794(1):17-22.JC

Abstract

A new analytical method for vigabatrin based on capillary electrophoretic separation and laser-induced fluorescence detection has been developed. 5-Carboxytetramethylrhodamine succinimidyl ester was used for precolumn derivatization of the non-fluorescent drug. Optimal separation and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH9.5) containing 10 mM sodium dodecyl sulfate and a green He-Ne laser (excitation at 543.5 nm, emission at 589 nm). The concentration limit of detection in aqueous solution was 24 nM. Combined with a simple cleanup procedure, this method can be applied to the determination of vigabatrin in human plasma. A calibration curve ranging from 1.5 to 200 microM shown to be linear. Both the within-day and day-to-day reproducibilities and accuracies were less then 14.3% and 4.9% respectively. The limit of detection of vigabatrin in plasma was about 0.13 microM

Authors+Show Affiliations

Chang SY
Department of Applied Chemistry, Chaoyang University of Technology, County, Wufeng, Taichung, Taiwan 413. ychang@cyut.edu.tw
Lin WC
No affiliation info available

MeSH

AnticonvulsantsCalibrationElectrophoresis, CapillaryHumansLasersReproducibility of ResultsSpectrometry, FluorescenceVigabatrin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12888194

Citation

Chang, Sarah Y., and Wen-Chung Lin. "Determination of Vigabatrin By Capillary Electrophoresis With Laser-induced Fluorescence Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 794, no. 1, 2003, pp. 17-22.
Chang SY, Lin WC. Determination of vigabatrin by capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2003;794(1):17-22.
Chang, S. Y., & Lin, W. C. (2003). Determination of vigabatrin by capillary electrophoresis with laser-induced fluorescence detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 794(1), 17-22.
Chang SY, Lin WC. Determination of Vigabatrin By Capillary Electrophoresis With Laser-induced Fluorescence Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Aug 25;794(1):17-22. PubMed PMID: 12888194.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of vigabatrin by capillary electrophoresis with laser-induced fluorescence detection. AU - Chang,Sarah Y, AU - Lin,Wen-Chung, PY - 2003/7/31/pubmed PY - 2004/7/17/medline PY - 2003/7/31/entrez SP - 17 EP - 22 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 794 IS - 1 N2 - A new analytical method for vigabatrin based on capillary electrophoretic separation and laser-induced fluorescence detection has been developed. 5-Carboxytetramethylrhodamine succinimidyl ester was used for precolumn derivatization of the non-fluorescent drug. Optimal separation and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH9.5) containing 10 mM sodium dodecyl sulfate and a green He-Ne laser (excitation at 543.5 nm, emission at 589 nm). The concentration limit of detection in aqueous solution was 24 nM. Combined with a simple cleanup procedure, this method can be applied to the determination of vigabatrin in human plasma. A calibration curve ranging from 1.5 to 200 microM shown to be linear. Both the within-day and day-to-day reproducibilities and accuracies were less then 14.3% and 4.9% respectively. The limit of detection of vigabatrin in plasma was about 0.13 microM SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/12888194/Determination_of_vigabatrin_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -