Tags

Type your tag names separated by a space and hit enter

[Determination of adenosine, rutin and quercetin in Carthamus tinctorius by HPCE].
Yao Xue Xue Bao. 2003 Apr; 38(4):283-5.YX

Abstract

AIM

To develop a method for determination of adenosine, rutin and quercetin in Carthamus tinctorius L. by high performance capillary electrophoresis(HPCE).

METHODS

A fused silica capillary (66.5 cm x 50 microns ID, an effective length of 58 cm) was used. The running buffer composed of 50 mmol.L-1 borax (pH 9.7) containing 18% methanol. The applied voltage was 24 kV and the capillary temperature was 20 degrees C. The detection wavelength was 210 nm. Rifampicin was used as internal standard.

RESULTS

A good linearity between peak area ratio of the common peak to the internal standard and the concentration was found in the range of 10-160 mg.L-1 for adenosine, 100-2,000 mg.L-1 for rutin and 100-1,600 mg.L-1 for quercetin (r > 0.998). The average recoveries were 98.5%-100.5%, 96.9%-99.5% and 99.1%-99.5% for adenosine, rutin and quercetin, respectively. The relative standard deviation was less than 6.5% (n = 5).

CONCLUSION

The method is simple, rapid and with satisfactory recoveries and good reproducibilities. It can be used to control the quality of Carthamus tinctorius.

Authors+Show Affiliations

College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

12889129

Citation

Sun, Yi, et al. "[Determination of Adenosine, Rutin and Quercetin in Carthamus Tinctorius By HPCE]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 38, no. 4, 2003, pp. 283-5.
Sun Y, Guo T, Sui Y, et al. [Determination of adenosine, rutin and quercetin in Carthamus tinctorius by HPCE]. Yao Xue Xue Bao. 2003;38(4):283-5.
Sun, Y., Guo, T., Sui, Y., & Li, F. M. (2003). [Determination of adenosine, rutin and quercetin in Carthamus tinctorius by HPCE]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 38(4), 283-5.
Sun Y, et al. [Determination of Adenosine, Rutin and Quercetin in Carthamus Tinctorius By HPCE]. Yao Xue Xue Bao. 2003;38(4):283-5. PubMed PMID: 12889129.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of adenosine, rutin and quercetin in Carthamus tinctorius by HPCE]. AU - Sun,Yi, AU - Guo,Tao, AU - Sui,Yin, AU - Li,Fa-mei, PY - 2003/8/2/pubmed PY - 2004/4/20/medline PY - 2003/8/2/entrez SP - 283 EP - 5 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 38 IS - 4 N2 - AIM: To develop a method for determination of adenosine, rutin and quercetin in Carthamus tinctorius L. by high performance capillary electrophoresis(HPCE). METHODS: A fused silica capillary (66.5 cm x 50 microns ID, an effective length of 58 cm) was used. The running buffer composed of 50 mmol.L-1 borax (pH 9.7) containing 18% methanol. The applied voltage was 24 kV and the capillary temperature was 20 degrees C. The detection wavelength was 210 nm. Rifampicin was used as internal standard. RESULTS: A good linearity between peak area ratio of the common peak to the internal standard and the concentration was found in the range of 10-160 mg.L-1 for adenosine, 100-2,000 mg.L-1 for rutin and 100-1,600 mg.L-1 for quercetin (r > 0.998). The average recoveries were 98.5%-100.5%, 96.9%-99.5% and 99.1%-99.5% for adenosine, rutin and quercetin, respectively. The relative standard deviation was less than 6.5% (n = 5). CONCLUSION: The method is simple, rapid and with satisfactory recoveries and good reproducibilities. It can be used to control the quality of Carthamus tinctorius. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/12889129/[Determination_of_adenosine_rutin_and_quercetin_in_Carthamus_tinctorius_by_HPCE]_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:12889129 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.