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Epstein-Barr virus encoded latent membrane protein 1 induces TRAF1 expression to promote anti-apoptosis activity via NF-kappaB signaling pathway in nasopharyngeal carcinoma.
Chin Med J (Engl). 2003 Jul; 116(7):1022-8.CM

Abstract

OBJECTIVES

To identify whether Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP1) can induce tumor necrosis factor receptor-associated factor 1 (TRAF1) expression and promote its anti-apoptosis activity via the NF-kappaB signaling pathway, and assess that LMP1 suppresses apoptosis in nasopharyngeal carcinoma (NPC).

METHODS

A stable transfected cell line HNE2-LMP1 was established by introducing LMP1 cDNA into HNE2 cells. Transactivation of TRAF1 was determined by luciferase reporter assay, while expression of TRAF1 mRNA was detected by RT-PCR and expression of TRAF1 protein and caspase 3 by Western blot analysis. Apoptosis activity was observed through fluorescence staining.

RESULTS

LMP1 induced TRAF1 expression in NPC cells and caused a decrease in apoptosis. This induction could be blocked by antisense LMP1. Moreover, LMP1-mediated induction of a TRAF1 promoter-driven reporter gene was significantly impaired when the kappaB site kappaB1 or kappaB5 was disrupted, whereas mutation of kappaB3 had only a minor effect on LMP1 dependent up-regulation of the reporter gene.

CONCLUSION

LMP1 induces TRAF1 expression and promotes its anti-apoptosis activity via the NF-kappaB signaling pathway, which may be one of the mechanisms that LMP1 uses to suppress apoptosis in NPC cells.

Authors+Show Affiliations

Department of Oral and Maxillofacial Surgery, Xiang Ya Hospital, Central Southern University, Changsha 410078, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12890376

Citation

Wang, Chengxing, et al. "Epstein-Barr Virus Encoded Latent Membrane Protein 1 Induces TRAF1 Expression to Promote Anti-apoptosis Activity Via NF-kappaB Signaling Pathway in Nasopharyngeal Carcinoma." Chinese Medical Journal, vol. 116, no. 7, 2003, pp. 1022-8.
Wang C, Ai M, Ren W, et al. Epstein-Barr virus encoded latent membrane protein 1 induces TRAF1 expression to promote anti-apoptosis activity via NF-kappaB signaling pathway in nasopharyngeal carcinoma. Chin Med J (Engl). 2003;116(7):1022-8.
Wang, C., Ai, M., Ren, W., Xiao, H., Li, X., Tang, F., Gu, H., Yi, W., Weng, X., Deng, X., & Cao, Y. (2003). Epstein-Barr virus encoded latent membrane protein 1 induces TRAF1 expression to promote anti-apoptosis activity via NF-kappaB signaling pathway in nasopharyngeal carcinoma. Chinese Medical Journal, 116(7), 1022-8.
Wang C, et al. Epstein-Barr Virus Encoded Latent Membrane Protein 1 Induces TRAF1 Expression to Promote Anti-apoptosis Activity Via NF-kappaB Signaling Pathway in Nasopharyngeal Carcinoma. Chin Med J (Engl). 2003;116(7):1022-8. PubMed PMID: 12890376.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epstein-Barr virus encoded latent membrane protein 1 induces TRAF1 expression to promote anti-apoptosis activity via NF-kappaB signaling pathway in nasopharyngeal carcinoma. AU - Wang,Chengxing, AU - Ai,Midan, AU - Ren,Wei, AU - Xiao,Hui, AU - Li,Xiaoyan, AU - Tang,Faqing, AU - Gu,Huanhua, AU - Yi,Wei, AU - Weng,Xinxian, AU - Deng,Xiyun, AU - Cao,Ya, PY - 2003/8/2/pubmed PY - 2003/10/18/medline PY - 2003/8/2/entrez SP - 1022 EP - 8 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 116 IS - 7 N2 - OBJECTIVES: To identify whether Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP1) can induce tumor necrosis factor receptor-associated factor 1 (TRAF1) expression and promote its anti-apoptosis activity via the NF-kappaB signaling pathway, and assess that LMP1 suppresses apoptosis in nasopharyngeal carcinoma (NPC). METHODS: A stable transfected cell line HNE2-LMP1 was established by introducing LMP1 cDNA into HNE2 cells. Transactivation of TRAF1 was determined by luciferase reporter assay, while expression of TRAF1 mRNA was detected by RT-PCR and expression of TRAF1 protein and caspase 3 by Western blot analysis. Apoptosis activity was observed through fluorescence staining. RESULTS: LMP1 induced TRAF1 expression in NPC cells and caused a decrease in apoptosis. This induction could be blocked by antisense LMP1. Moreover, LMP1-mediated induction of a TRAF1 promoter-driven reporter gene was significantly impaired when the kappaB site kappaB1 or kappaB5 was disrupted, whereas mutation of kappaB3 had only a minor effect on LMP1 dependent up-regulation of the reporter gene. CONCLUSION: LMP1 induces TRAF1 expression and promotes its anti-apoptosis activity via the NF-kappaB signaling pathway, which may be one of the mechanisms that LMP1 uses to suppress apoptosis in NPC cells. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/12890376/Epstein_Barr_virus_encoded_latent_membrane_protein_1_induces_TRAF1_expression_to_promote_anti_apoptosis_activity_via_NF_kappaB_signaling_pathway_in_nasopharyngeal_carcinoma_ L2 - https://www.diseaseinfosearch.org/result/5110 DB - PRIME DP - Unbound Medicine ER -