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Low-fat diet impairs postresection intestinal adaptation in a rat model of short bowel syndrome.
J Pediatr Surg. 2003 Aug; 38(8):1182-7.JP

Abstract

BACKGROUND

Low-fat diets (LFD) are utilized frequently in patients with short bowel syndrome (SBS). The purpose of this study was to investigate the effects of LFD on intestinal adaptation, enterocyte proliferation, and enterocyte cell death in a rat model of SBS.

METHODS

Adult male Sprague-Dawley rats were divided into 3 experimental groups: Sham-NC rats underwent bowel transection and reanastomosis and were fed normal chow (NC), SBS-NC rats underwent 75% small bowel resection and were fed NC, and SBS-rats were fed a low-fat diet (SBS-LFD). Parameters of intestinal adaptation, enterocyte proliferation, and enterocyte apoptosis were determined on day 14 after operation.

RESULTS

SBS-NC rats showed a significant increase (v Sham-NC) in jejunal and ileal bowel and mucosal weight, mucosal DNA and protein, villus height, and crypt depth. A significant 67% increase in crypt cell proliferation rate and 265% increase in villus enterocyte apoptosis was seen in the ileum of SBS-NC rats compared with control animals (P <.05). SBS-LFD animals showed lower ileal mucosal weight (29%; P <.05), jejunal crypt depth (20%; P <.05), and ileal villus height (21%; P <.05). A significant decrease in villus apoptosis in jejunum (74%; P <.05) and ileum (67%; P <.05) and a decrease in cell proliferation in ileum (35%; P <.05) was seen also after exposure to LFD compared with SBS-NC.

CONCLUSIONS

In a rat model of SBS, early LFD appears to inhibit parameters of intestinal adaptation. A possible mechanisms for this effect may be decreased cell proliferation. Decreased enterocyte loss via apoptosis, found in this study, may reflect a reduced number of enterocyte.

Authors+Show Affiliations

Department of Surgery, Rappaport Faculty of Medicine, Technion, Carmel Medical Center, Haifa, Israel.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12891489

Citation

Sukhotnik, Igor, et al. "Low-fat Diet Impairs Postresection Intestinal Adaptation in a Rat Model of Short Bowel Syndrome." Journal of Pediatric Surgery, vol. 38, no. 8, 2003, pp. 1182-7.
Sukhotnik I, Shiloni E, Krausz MM, et al. Low-fat diet impairs postresection intestinal adaptation in a rat model of short bowel syndrome. J Pediatr Surg. 2003;38(8):1182-7.
Sukhotnik, I., Shiloni, E., Krausz, M. M., Yakirevich, E., Sabo, E., Mogilner, J., Coran, A. G., & Harmon, C. M. (2003). Low-fat diet impairs postresection intestinal adaptation in a rat model of short bowel syndrome. Journal of Pediatric Surgery, 38(8), 1182-7.
Sukhotnik I, et al. Low-fat Diet Impairs Postresection Intestinal Adaptation in a Rat Model of Short Bowel Syndrome. J Pediatr Surg. 2003;38(8):1182-7. PubMed PMID: 12891489.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Low-fat diet impairs postresection intestinal adaptation in a rat model of short bowel syndrome. AU - Sukhotnik,Igor, AU - Shiloni,Eitan, AU - Krausz,Michael M, AU - Yakirevich,Evgeny, AU - Sabo,Edmund, AU - Mogilner,Jorge, AU - Coran,Arnold G, AU - Harmon,Carroll M, PY - 2003/8/2/pubmed PY - 2003/11/5/medline PY - 2003/8/2/entrez SP - 1182 EP - 7 JF - Journal of pediatric surgery JO - J Pediatr Surg VL - 38 IS - 8 N2 - BACKGROUND: Low-fat diets (LFD) are utilized frequently in patients with short bowel syndrome (SBS). The purpose of this study was to investigate the effects of LFD on intestinal adaptation, enterocyte proliferation, and enterocyte cell death in a rat model of SBS. METHODS: Adult male Sprague-Dawley rats were divided into 3 experimental groups: Sham-NC rats underwent bowel transection and reanastomosis and were fed normal chow (NC), SBS-NC rats underwent 75% small bowel resection and were fed NC, and SBS-rats were fed a low-fat diet (SBS-LFD). Parameters of intestinal adaptation, enterocyte proliferation, and enterocyte apoptosis were determined on day 14 after operation. RESULTS: SBS-NC rats showed a significant increase (v Sham-NC) in jejunal and ileal bowel and mucosal weight, mucosal DNA and protein, villus height, and crypt depth. A significant 67% increase in crypt cell proliferation rate and 265% increase in villus enterocyte apoptosis was seen in the ileum of SBS-NC rats compared with control animals (P <.05). SBS-LFD animals showed lower ileal mucosal weight (29%; P <.05), jejunal crypt depth (20%; P <.05), and ileal villus height (21%; P <.05). A significant decrease in villus apoptosis in jejunum (74%; P <.05) and ileum (67%; P <.05) and a decrease in cell proliferation in ileum (35%; P <.05) was seen also after exposure to LFD compared with SBS-NC. CONCLUSIONS: In a rat model of SBS, early LFD appears to inhibit parameters of intestinal adaptation. A possible mechanisms for this effect may be decreased cell proliferation. Decreased enterocyte loss via apoptosis, found in this study, may reflect a reduced number of enterocyte. SN - 1531-5037 UR - https://www.unboundmedicine.com/medline/citation/12891489/Low_fat_diet_impairs_postresection_intestinal_adaptation_in_a_rat_model_of_short_bowel_syndrome_ DB - PRIME DP - Unbound Medicine ER -