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Fractionation of grape tannins and analysis by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry.
Phytochem Anal. 2003 Jul-Aug; 14(4):202-8.PA

Abstract

Polymeric tannins, extracted from grape berries (Gamay variety), were fractionated according to their mean degree of polymerisation (mDP) on a styrene-divinylbenzene phase eluted with a gradient of methanol:chloroform. Increasing the percentage of methanol led to the solubilisation of higher molecular weight tannins. The mean mDP of each collected fraction was determined by acid-catalysed degradation in the presence of a nucleophilic reagent. The fractionation method produced a linear gradient of mDP varying between 1.84 and 19.34. The fractions were partially characterised by matrix assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). The spectra showed a complex mixture of proanthocyanidins and galloylated proanthocyanidins up to 4000 amu.

Authors+Show Affiliations

Institut de Chimie, Université de Neuchâtel, Av. de Bellevaux 51, CH-2000 Neuchâtel, Switzerland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12892414

Citation

Perret, Camille, et al. "Fractionation of Grape Tannins and Analysis By Matrix-assisted Laser Desorption/ionisation Time-of-flight Mass Spectrometry." Phytochemical Analysis : PCA, vol. 14, no. 4, 2003, pp. 202-8.
Perret C, Pezet R, Tabacchi R. Fractionation of grape tannins and analysis by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Phytochem Anal. 2003;14(4):202-8.
Perret, C., Pezet, R., & Tabacchi, R. (2003). Fractionation of grape tannins and analysis by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Phytochemical Analysis : PCA, 14(4), 202-8.
Perret C, Pezet R, Tabacchi R. Fractionation of Grape Tannins and Analysis By Matrix-assisted Laser Desorption/ionisation Time-of-flight Mass Spectrometry. Phytochem Anal. 2003 Jul-Aug;14(4):202-8. PubMed PMID: 12892414.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fractionation of grape tannins and analysis by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. AU - Perret,Camille, AU - Pezet,Roger, AU - Tabacchi,Raphael, PY - 2003/8/2/pubmed PY - 2003/9/25/medline PY - 2003/8/2/entrez SP - 202 EP - 8 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 14 IS - 4 N2 - Polymeric tannins, extracted from grape berries (Gamay variety), were fractionated according to their mean degree of polymerisation (mDP) on a styrene-divinylbenzene phase eluted with a gradient of methanol:chloroform. Increasing the percentage of methanol led to the solubilisation of higher molecular weight tannins. The mean mDP of each collected fraction was determined by acid-catalysed degradation in the presence of a nucleophilic reagent. The fractionation method produced a linear gradient of mDP varying between 1.84 and 19.34. The fractions were partially characterised by matrix assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). The spectra showed a complex mixture of proanthocyanidins and galloylated proanthocyanidins up to 4000 amu. SN - 0958-0344 UR - https://www.unboundmedicine.com/medline/citation/12892414/Fractionation_of_grape_tannins_and_analysis_by_matrix_assisted_laser_desorption/ionisation_time_of_flight_mass_spectrometry_ L2 - https://doi.org/10.1002/pca.705 DB - PRIME DP - Unbound Medicine ER -