Tags

Type your tag names separated by a space and hit enter

A simple differential pulse polarographic method for the determination of thymoquinone in black seed oil.
Phytochem Anal. 2003 Jul-Aug; 14(4):224-7.PA

Abstract

A reliable and simple differential pulse polarographic method is described for the determination of thymoquinone in black seed oil. The polarographic behaviour of thymoquinone was examined in various buffer systems over the pH range 5.0-10.0. Thymoquinone is reduced in a single, reversible peak at the dropping mercury electrode. The differential pulse polarogram showed a distinct peak in Sörensen buffer:methanol (3:7, v/v; pH 8.5) at a peak potential of -0.095 V (vs. silver/silver chloride electrode), and a plot of peak height against concentration was found to be linear over the range 0.2-15.0 microg/mL (R = 0.9998). The limit of detection was calculated to be 0.054 microg/mL. The polarographic method has been applied to determine thymoquinone in two black seed oil preparations available on the Austrian pharmaceutical market.

Authors+Show Affiliations

Institut für Pharmazeutische Chemie und Pharmazeutische Technologie der Karl-Franzens-Universität Graz, Schubertstrasse 1, A-8010 Graz, Austria. astrid.ortner@kfunigraz.ac.atNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12892418

Citation

Michelitsch, Astrid, and Anna Rittmannsberger. "A Simple Differential Pulse Polarographic Method for the Determination of Thymoquinone in Black Seed Oil." Phytochemical Analysis : PCA, vol. 14, no. 4, 2003, pp. 224-7.
Michelitsch A, Rittmannsberger A. A simple differential pulse polarographic method for the determination of thymoquinone in black seed oil. Phytochem Anal. 2003;14(4):224-7.
Michelitsch, A., & Rittmannsberger, A. (2003). A simple differential pulse polarographic method for the determination of thymoquinone in black seed oil. Phytochemical Analysis : PCA, 14(4), 224-7.
Michelitsch A, Rittmannsberger A. A Simple Differential Pulse Polarographic Method for the Determination of Thymoquinone in Black Seed Oil. Phytochem Anal. 2003 Jul-Aug;14(4):224-7. PubMed PMID: 12892418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A simple differential pulse polarographic method for the determination of thymoquinone in black seed oil. AU - Michelitsch,Astrid, AU - Rittmannsberger,Anna, PY - 2003/8/2/pubmed PY - 2003/9/25/medline PY - 2003/8/2/entrez SP - 224 EP - 7 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 14 IS - 4 N2 - A reliable and simple differential pulse polarographic method is described for the determination of thymoquinone in black seed oil. The polarographic behaviour of thymoquinone was examined in various buffer systems over the pH range 5.0-10.0. Thymoquinone is reduced in a single, reversible peak at the dropping mercury electrode. The differential pulse polarogram showed a distinct peak in Sörensen buffer:methanol (3:7, v/v; pH 8.5) at a peak potential of -0.095 V (vs. silver/silver chloride electrode), and a plot of peak height against concentration was found to be linear over the range 0.2-15.0 microg/mL (R = 0.9998). The limit of detection was calculated to be 0.054 microg/mL. The polarographic method has been applied to determine thymoquinone in two black seed oil preparations available on the Austrian pharmaceutical market. SN - 0958-0344 UR - https://www.unboundmedicine.com/medline/citation/12892418/A_simple_differential_pulse_polarographic_method_for_the_determination_of_thymoquinone_in_black_seed_oil_ L2 - https://doi.org/10.1002/pca.707 DB - PRIME DP - Unbound Medicine ER -