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Regulation of intestinal NaPi-IIb cotransporter gene expression by estrogen.
Am J Physiol Gastrointest Liver Physiol. 2003 Dec; 285(6):G1317-24.AJ

Abstract

The current experiments were designed to study the effect of beta-estradiol on type IIb sodium-coupled phosphate (NaPi-IIb) cotransporter gene expression. Uptake studies with intestinal brush-border membrane vesicles (BBMV) showed that estrogen treatment increased sodium-dependent phosphate absorption by approximately 45% in rat intestine. Northern blot analysis indicated that NaPi-IIb mRNA expression was increased by approximately 50% after estrogen treatment. Western blot analysis also detected an increase in BBMV NaPi-IIb protein expression in estrogen-treated rats. In human intestinal Caco-2 cells, NaPi-IIb mRNA abundance was increased approximately 60% after estrogen treatment, and this increase could be abolished by inhibition of gene transcription. Transfection studies with human NaPi-IIb promoter reporter constructs showed that the promoter was responsive to estrogen treatment. These studies demonstrate for the first time that estrogen stimulates intestinal sodium-dependent phosphate absorption in female rats. This stimulation is associated with increased NaPi-IIb mRNA and protein expression. Thus the effect of estrogen on intestinal Pi absorption may be partially due to activation of NaPi-IIb gene transcription.

Authors+Show Affiliations

Department of Pediatrics, Steele Memorial Children's Research Center, University of Arizona Health Sciences Center, Tucson, Arizona 85724, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12893629

Citation

Xu, Hua, et al. "Regulation of Intestinal NaPi-IIb Cotransporter Gene Expression By Estrogen." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 285, no. 6, 2003, pp. G1317-24.
Xu H, Uno JK, Inouye M, et al. Regulation of intestinal NaPi-IIb cotransporter gene expression by estrogen. Am J Physiol Gastrointest Liver Physiol. 2003;285(6):G1317-24.
Xu, H., Uno, J. K., Inouye, M., Xu, L., Drees, J. B., Collins, J. F., & Ghishan, F. K. (2003). Regulation of intestinal NaPi-IIb cotransporter gene expression by estrogen. American Journal of Physiology. Gastrointestinal and Liver Physiology, 285(6), G1317-24.
Xu H, et al. Regulation of Intestinal NaPi-IIb Cotransporter Gene Expression By Estrogen. Am J Physiol Gastrointest Liver Physiol. 2003;285(6):G1317-24. PubMed PMID: 12893629.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of intestinal NaPi-IIb cotransporter gene expression by estrogen. AU - Xu,Hua, AU - Uno,Jennifer K, AU - Inouye,Michael, AU - Xu,Liping, AU - Drees,Jason B, AU - Collins,James F, AU - Ghishan,Fayez K, Y1 - 2003/07/31/ PY - 2003/8/2/pubmed PY - 2004/1/9/medline PY - 2003/8/2/entrez SP - G1317 EP - 24 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am J Physiol Gastrointest Liver Physiol VL - 285 IS - 6 N2 - The current experiments were designed to study the effect of beta-estradiol on type IIb sodium-coupled phosphate (NaPi-IIb) cotransporter gene expression. Uptake studies with intestinal brush-border membrane vesicles (BBMV) showed that estrogen treatment increased sodium-dependent phosphate absorption by approximately 45% in rat intestine. Northern blot analysis indicated that NaPi-IIb mRNA expression was increased by approximately 50% after estrogen treatment. Western blot analysis also detected an increase in BBMV NaPi-IIb protein expression in estrogen-treated rats. In human intestinal Caco-2 cells, NaPi-IIb mRNA abundance was increased approximately 60% after estrogen treatment, and this increase could be abolished by inhibition of gene transcription. Transfection studies with human NaPi-IIb promoter reporter constructs showed that the promoter was responsive to estrogen treatment. These studies demonstrate for the first time that estrogen stimulates intestinal sodium-dependent phosphate absorption in female rats. This stimulation is associated with increased NaPi-IIb mRNA and protein expression. Thus the effect of estrogen on intestinal Pi absorption may be partially due to activation of NaPi-IIb gene transcription. SN - 0193-1857 UR - https://www.unboundmedicine.com/medline/citation/12893629/Regulation_of_intestinal_NaPi_IIb_cotransporter_gene_expression_by_estrogen_ L2 - https://journals.physiology.org/doi/10.1152/ajpgi.00172.2003?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -