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Human CD8+CD25+ thymocytes share phenotypic and functional features with CD4+CD25+ regulatory thymocytes.
Blood. 2003 Dec 01; 102(12):4107-14.Blood

Abstract

CD8+CD25+ cells, which expressed high levels of Foxp3, glucocorticoid-induced tumor necrosis factor receptor (GITR), CCR8, tumor necrosis factor receptor 2 (TNFR2), and cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) mRNAs, were identified in the fibrous septa and medullary areas of human thymus. Activated CD8+CD25+ thymocytes did not produce cytokines, but most of them expressed surface CTLA-4 and transforming growth factor beta1 (TGF-beta1). Like CD4+CD25+, CD8+CD25+ thymocytes suppressed the proliferation of autologous CD25-T cells via a contact-dependent mechanism. The suppressive activity of CD8+CD25+ thymocytes was abrogated by a mixture of anti-CTLA-4 and anti-TGF-beta1 antibodies and it was mediated by their ability to inhibit the expression of the interleukin 2 receptor alpha chain on target T cells. These results demonstrate the existence of a subset of human CD8+CD25+ thymocytes sharing phenotype, functional features, and mechanism of action with CD4+CD25+ T regulatory cells.

Links

Publisher Full Text
linkinghub.elsevier.com
ashpublications.org

Authors+Show Affiliations

Cosmi L
Dipartimento di Medicina Interna, Viale Morgagni 85, Firenze 50134, Italy.
Liotta F
No affiliation info available
Lazzeri E
No affiliation info available
Francalanci M
No affiliation info available
Angeli R
No affiliation info available
Mazzinghi B
No affiliation info available
Santarlasci V
No affiliation info available
Manetti R
No affiliation info available
Vanini V
No affiliation info available
Romagnani P
No affiliation info available
Maggi E
No affiliation info available
Romagnani S
No affiliation info available
Annunziato F
No affiliation info available

MeSH

Antigens, CDAntigens, DifferentiationCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCTLA-4 AntigenCytokinesFlow CytometryHumansImmunohistochemistryImmunophenotypingInterleukin-2Lymphocyte ActivationMembrane ProteinsReceptors, Interleukin-2T-Lymphocyte SubsetsThymus GlandTransforming Growth Factor betaTransforming Growth Factor beta1

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12893750

Citation

Cosmi, Lorenzo, et al. "Human CD8+CD25+ Thymocytes Share Phenotypic and Functional Features With CD4+CD25+ Regulatory Thymocytes." Blood, vol. 102, no. 12, 2003, pp. 4107-14.
Cosmi L, Liotta F, Lazzeri E, et al. Human CD8+CD25+ thymocytes share phenotypic and functional features with CD4+CD25+ regulatory thymocytes. Blood. 2003;102(12):4107-14.
Cosmi, L., Liotta, F., Lazzeri, E., Francalanci, M., Angeli, R., Mazzinghi, B., Santarlasci, V., Manetti, R., Vanini, V., Romagnani, P., Maggi, E., Romagnani, S., & Annunziato, F. (2003). Human CD8+CD25+ thymocytes share phenotypic and functional features with CD4+CD25+ regulatory thymocytes. Blood, 102(12), 4107-14.
Cosmi L, et al. Human CD8+CD25+ Thymocytes Share Phenotypic and Functional Features With CD4+CD25+ Regulatory Thymocytes. Blood. 2003 Dec 1;102(12):4107-14. PubMed PMID: 12893750.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human CD8+CD25+ thymocytes share phenotypic and functional features with CD4+CD25+ regulatory thymocytes. AU - Cosmi,Lorenzo, AU - Liotta,Francesco, AU - Lazzeri,Elena, AU - Francalanci,Michela, AU - Angeli,Roberta, AU - Mazzinghi,Benedetta, AU - Santarlasci,Veronica, AU - Manetti,Roberto, AU - Vanini,Vittorio, AU - Romagnani,Paola, AU - Maggi,Enrico, AU - Romagnani,Sergio, AU - Annunziato,Francesco, Y1 - 2003/07/31/ PY - 2003/8/2/pubmed PY - 2004/1/15/medline PY - 2003/8/2/entrez SP - 4107 EP - 14 JF - Blood JO - Blood VL - 102 IS - 12 N2 - CD8+CD25+ cells, which expressed high levels of Foxp3, glucocorticoid-induced tumor necrosis factor receptor (GITR), CCR8, tumor necrosis factor receptor 2 (TNFR2), and cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) mRNAs, were identified in the fibrous septa and medullary areas of human thymus. Activated CD8+CD25+ thymocytes did not produce cytokines, but most of them expressed surface CTLA-4 and transforming growth factor beta1 (TGF-beta1). Like CD4+CD25+, CD8+CD25+ thymocytes suppressed the proliferation of autologous CD25-T cells via a contact-dependent mechanism. The suppressive activity of CD8+CD25+ thymocytes was abrogated by a mixture of anti-CTLA-4 and anti-TGF-beta1 antibodies and it was mediated by their ability to inhibit the expression of the interleukin 2 receptor alpha chain on target T cells. These results demonstrate the existence of a subset of human CD8+CD25+ thymocytes sharing phenotype, functional features, and mechanism of action with CD4+CD25+ T regulatory cells. SN - 0006-4971 UR - https://www.unboundmedicine.com/medline/citation/12893750/Human_CD8+CD25+_thymocytes_share_phenotypic_and_functional_features_with_CD4+CD25+_regulatory_thymocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/2003-04-1320 DB - PRIME DP - Unbound Medicine ER -