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Fate of melanocytes during development of the hair follicle pigmentary unit.
J Investig Dermatol Symp Proc. 2003 Jun; 8(1):76-9.JI

Abstract

During hair follicle morphogenesis, melanocyte precursors migrate into developing hair follicles and give rise to differentiated melanocytes that actively produce and transport pigment into the keratinocytes that form the hair shaft; however, patterns of melanocyte proliferation and differentiation during formation of the hair pigmentation unit remain to be elucidated. Using multicolor confocal microscopy and double immunofluorescence of melanogenic proteins (tyrosinase-related proteins 1 and 2, tyrosinase) and the proliferative marker Ki67, we have studied melanocyte development in C57BL/6 mouse embryonic hair follicles. Proliferating melanocyte precursors (tyrosinase-related protein-2/Ki67+ cells) are seen in the hair follicles at stages 1-2 of morphogenesis, as follicular invagination begins. In stage 3-4 hair follicles, the majority of intrafollicular melanocytes remain tyrosinase-related protein-2+ and Ki67+, whereas some located adjacent to the forming dermal papilla begin to express tyrosinase-related protein-1, an early marker of differentiation. Melanin granules appear in stage 5 hair follicles coincident with tyrosinase expression in nonproliferating tyrosinase-related protein-2+/tyrosinase-related protein-1+ melanocytes. Stage 6-8 hair follicles, those actively producing hair, show nonproliferating tyrosinase-related protein-2+ melanocytes in the bulge area, tyrosinase-related protein-2+/tyrosinase-related protein-1+ melanocytes in the outer root sheath, and tyrosinase-related protein-2+/tyrosinase-related protein-1+/tyrosinase+ melanocytes above the dermal papilla. These data suggest that melanocyte precursor cells proliferate extensively at the onset of follicle development. Progeny of these cells migrate down the developing follicle, differentiating further until reaching the area immediately above the dermal papilla, where fully differentiated nonproliferative melanin-producing melanocytes persist, contributing pigment to the growing hair shaft.

Authors+Show Affiliations

Botchkareva NV
Department of Dermatology, Boston University School of Medicine, Boston, MA 02118, USA.
Botchkarev VA
No affiliation info available
Gilchrest BA
No affiliation info available

MeSH

AnimalsCell DifferentiationCell DivisionEmbryonic and Fetal DevelopmentHair FollicleImmunohistochemistryIntramolecular OxidoreductasesKi-67 AntigenMelaninsMelanocytesMembrane GlycoproteinsMiceMice, Inbred C57BLMicroscopy, ConfocalMonophenol MonooxygenaseOxidoreductasesPigmentationProteinsStem Cells

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12894999

Citation

Botchkareva, Natalia V., et al. "Fate of Melanocytes During Development of the Hair Follicle Pigmentary Unit." The Journal of Investigative Dermatology. Symposium Proceedings, vol. 8, no. 1, 2003, pp. 76-9.
Botchkareva NV, Botchkarev VA, Gilchrest BA. Fate of melanocytes during development of the hair follicle pigmentary unit. J Investig Dermatol Symp Proc. 2003;8(1):76-9.
Botchkareva, N. V., Botchkarev, V. A., & Gilchrest, B. A. (2003). Fate of melanocytes during development of the hair follicle pigmentary unit. The Journal of Investigative Dermatology. Symposium Proceedings, 8(1), 76-9.
Botchkareva NV, Botchkarev VA, Gilchrest BA. Fate of Melanocytes During Development of the Hair Follicle Pigmentary Unit. J Investig Dermatol Symp Proc. 2003;8(1):76-9. PubMed PMID: 12894999.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fate of melanocytes during development of the hair follicle pigmentary unit. AU - Botchkareva,Natalia V, AU - Botchkarev,Vladimir A, AU - Gilchrest,Barbara A, PY - 2003/8/5/pubmed PY - 2004/3/5/medline PY - 2003/8/5/entrez SP - 76 EP - 9 JF - The journal of investigative dermatology. Symposium proceedings JO - J Investig Dermatol Symp Proc VL - 8 IS - 1 N2 - During hair follicle morphogenesis, melanocyte precursors migrate into developing hair follicles and give rise to differentiated melanocytes that actively produce and transport pigment into the keratinocytes that form the hair shaft; however, patterns of melanocyte proliferation and differentiation during formation of the hair pigmentation unit remain to be elucidated. Using multicolor confocal microscopy and double immunofluorescence of melanogenic proteins (tyrosinase-related proteins 1 and 2, tyrosinase) and the proliferative marker Ki67, we have studied melanocyte development in C57BL/6 mouse embryonic hair follicles. Proliferating melanocyte precursors (tyrosinase-related protein-2/Ki67+ cells) are seen in the hair follicles at stages 1-2 of morphogenesis, as follicular invagination begins. In stage 3-4 hair follicles, the majority of intrafollicular melanocytes remain tyrosinase-related protein-2+ and Ki67+, whereas some located adjacent to the forming dermal papilla begin to express tyrosinase-related protein-1, an early marker of differentiation. Melanin granules appear in stage 5 hair follicles coincident with tyrosinase expression in nonproliferating tyrosinase-related protein-2+/tyrosinase-related protein-1+ melanocytes. Stage 6-8 hair follicles, those actively producing hair, show nonproliferating tyrosinase-related protein-2+ melanocytes in the bulge area, tyrosinase-related protein-2+/tyrosinase-related protein-1+ melanocytes in the outer root sheath, and tyrosinase-related protein-2+/tyrosinase-related protein-1+/tyrosinase+ melanocytes above the dermal papilla. These data suggest that melanocyte precursor cells proliferate extensively at the onset of follicle development. Progeny of these cells migrate down the developing follicle, differentiating further until reaching the area immediately above the dermal papilla, where fully differentiated nonproliferative melanin-producing melanocytes persist, contributing pigment to the growing hair shaft. SN - 1087-0024 UR - https://www.unboundmedicine.com/medline/citation/12894999/Fate_of_melanocytes_during_development_of_the_hair_follicle_pigmentary_unit_ DB - PRIME DP - Unbound Medicine ER -