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Sensitivity of the acoustic waveguide biosensor to protein binding as a function of the waveguide properties.
Biosens Bioelectron. 2003 Oct 01; 18(11):1399-406.BB

Abstract

The aim of this work is to study the effect of operating frequency, piezoelectric substrate and waveguide layer thickness on the sensitivity of the acoustic waveguide sensor during the specific binding of an antibody by a protein. Shear horizontal (SH) wave devices consisting of (a) a LiTaO3 substrate operating at 104 MHz, (b) a quartz substrate operating at 108 MHz and (c) a quartz substrate operating at 155 MHz were coated with a photoresist polymer layer in order to produce acoustic waveguide devices supporting a Love wave. The effect of the thickness of the polymer layer on the Love wave was assessed by measuring the amplitude and phase of the wave before and after coating. The sensitivity of the above three biosensors was compared during the detection of the specific binding of different concentrations of Immunoglobulin G in the range of 0.7-667 nM to a protein A modified surface. Results indicate that the thickness of the polymer guiding layer is critical for obtaining the maximum sensitivity for a given geometry but a trade-off has to be made between the theoretically determined optimum thickness for waveguiding and the device insertion loss. It was also found that increasing the frequency of operation results in a further increase in the device sensitivity to protein detection.

Authors+Show Affiliations

Gizeli E
Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK. e.gizeli@biotech.cam.ac.uk
Bender F
No affiliation info available
Rasmusson A
No affiliation info available
Saha K
No affiliation info available
Josse F
No affiliation info available
Cernosek R
No affiliation info available

MeSH

AcousticsAdsorptionBiosensing TechniquesEquipment DesignEquipment Failure AnalysisImmunoassayImmunoglobulin GProtein BindingRadio WavesReproducibility of ResultsSensitivity and SpecificityStaphylococcal Protein A

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Validation Study

Language

eng

PubMed ID

12896842

Citation

Gizeli, E, et al. "Sensitivity of the Acoustic Waveguide Biosensor to Protein Binding as a Function of the Waveguide Properties." Biosensors & Bioelectronics, vol. 18, no. 11, 2003, pp. 1399-406.
Gizeli E, Bender F, Rasmusson A, et al. Sensitivity of the acoustic waveguide biosensor to protein binding as a function of the waveguide properties. Biosens Bioelectron. 2003;18(11):1399-406.
Gizeli, E., Bender, F., Rasmusson, A., Saha, K., Josse, F., & Cernosek, R. (2003). Sensitivity of the acoustic waveguide biosensor to protein binding as a function of the waveguide properties. Biosensors & Bioelectronics, 18(11), 1399-406.
Gizeli E, et al. Sensitivity of the Acoustic Waveguide Biosensor to Protein Binding as a Function of the Waveguide Properties. Biosens Bioelectron. 2003 Oct 1;18(11):1399-406. PubMed PMID: 12896842.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitivity of the acoustic waveguide biosensor to protein binding as a function of the waveguide properties. AU - Gizeli,E, AU - Bender,F, AU - Rasmusson,A, AU - Saha,K, AU - Josse,F, AU - Cernosek,R, PY - 2003/8/5/pubmed PY - 2004/5/12/medline PY - 2003/8/5/entrez SP - 1399 EP - 406 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 18 IS - 11 N2 - The aim of this work is to study the effect of operating frequency, piezoelectric substrate and waveguide layer thickness on the sensitivity of the acoustic waveguide sensor during the specific binding of an antibody by a protein. Shear horizontal (SH) wave devices consisting of (a) a LiTaO3 substrate operating at 104 MHz, (b) a quartz substrate operating at 108 MHz and (c) a quartz substrate operating at 155 MHz were coated with a photoresist polymer layer in order to produce acoustic waveguide devices supporting a Love wave. The effect of the thickness of the polymer layer on the Love wave was assessed by measuring the amplitude and phase of the wave before and after coating. The sensitivity of the above three biosensors was compared during the detection of the specific binding of different concentrations of Immunoglobulin G in the range of 0.7-667 nM to a protein A modified surface. Results indicate that the thickness of the polymer guiding layer is critical for obtaining the maximum sensitivity for a given geometry but a trade-off has to be made between the theoretically determined optimum thickness for waveguiding and the device insertion loss. It was also found that increasing the frequency of operation results in a further increase in the device sensitivity to protein detection. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/12896842/Sensitivity_of_the_acoustic_waveguide_biosensor_to_protein_binding_as_a_function_of_the_waveguide_properties_ DB - PRIME DP - Unbound Medicine ER -