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Genetic and physical mapping of sequence-specific amplified polymorphic (SSAP) markers on the 1RS chromosome arm of rye in a wheat background.
Theor Appl Genet. 2003 Nov; 107(7):1271-7.TA

Abstract

Three rye-specific repeated sequences, pSc10C, pSc20H and R173-1, were used to design sequence-specific anchored primers. These primers and 16 restriction site-specific adaptor primers were used in all possible combinations to establish sequence-specific amplified polymorphic (SSAP) markers for the 1RS chromosome arm of rye in a wheat background. Thirty 1RS-specific SSAP markers were detected in 19 primer combinations. Along with six markers localised previously on 1RS, 26 of the SSAP markers were mapped genetically in wheat genotypes carrying recombinant 1BL.1RS translocations. A clear decrease in recombination frequency from distal to proximal regions was observed. Wheat-rye addition lines for the 1R chromosome with different-sized deletions of the short arm were used to physically localise these markers. Physical mapping suggested an even distribution of the SSAP markers along the total length of the 1RS chromosome arm.

Authors+Show Affiliations

Nagy ED
Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvásár, 2462, Brunszvik 2, Hungary.
Lelley T
No affiliation info available

MeSH

Chromosome MappingChromosomes, PlantDNA, PlantGenetic MarkersGenome, PlantRandom Amplified Polymorphic DNA TechniqueSecaleTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12898027

Citation

Nagy, E D., and T Lelley. "Genetic and Physical Mapping of Sequence-specific Amplified Polymorphic (SSAP) Markers On the 1RS Chromosome Arm of Rye in a Wheat Background." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 107, no. 7, 2003, pp. 1271-7.
Nagy ED, Lelley T. Genetic and physical mapping of sequence-specific amplified polymorphic (SSAP) markers on the 1RS chromosome arm of rye in a wheat background. Theor Appl Genet. 2003;107(7):1271-7.
Nagy, E. D., & Lelley, T. (2003). Genetic and physical mapping of sequence-specific amplified polymorphic (SSAP) markers on the 1RS chromosome arm of rye in a wheat background. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 107(7), 1271-7.
Nagy ED, Lelley T. Genetic and Physical Mapping of Sequence-specific Amplified Polymorphic (SSAP) Markers On the 1RS Chromosome Arm of Rye in a Wheat Background. Theor Appl Genet. 2003;107(7):1271-7. PubMed PMID: 12898027.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic and physical mapping of sequence-specific amplified polymorphic (SSAP) markers on the 1RS chromosome arm of rye in a wheat background. AU - Nagy,E D, AU - Lelley,T, Y1 - 2003/08/01/ PY - 2002/12/31/received PY - 2003/04/16/accepted PY - 2003/8/5/pubmed PY - 2004/2/18/medline PY - 2003/8/5/entrez SP - 1271 EP - 7 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 107 IS - 7 N2 - Three rye-specific repeated sequences, pSc10C, pSc20H and R173-1, were used to design sequence-specific anchored primers. These primers and 16 restriction site-specific adaptor primers were used in all possible combinations to establish sequence-specific amplified polymorphic (SSAP) markers for the 1RS chromosome arm of rye in a wheat background. Thirty 1RS-specific SSAP markers were detected in 19 primer combinations. Along with six markers localised previously on 1RS, 26 of the SSAP markers were mapped genetically in wheat genotypes carrying recombinant 1BL.1RS translocations. A clear decrease in recombination frequency from distal to proximal regions was observed. Wheat-rye addition lines for the 1R chromosome with different-sized deletions of the short arm were used to physically localise these markers. Physical mapping suggested an even distribution of the SSAP markers along the total length of the 1RS chromosome arm. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/12898027/Genetic_and_physical_mapping_of_sequence_specific_amplified_polymorphic__SSAP__markers_on_the_1RS_chromosome_arm_of_rye_in_a_wheat_background_ DB - PRIME DP - Unbound Medicine ER -