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Expression of GABA transporters, GAT-1 and GAT-3, in the cerebral cortex and thalamus of the rat during postnatal development.
Cell Tissue Res 2003; 313(3):245-57CT

Abstract

The cellular and subcellular localization of two GABA transporters, GAT-1 and GAT-3, was investigated using immunocytochemical methods in the rat cerebral cortex and thalamus during postnatal development. The distribution of the transporters is compared with that of the neuronal marker GABA, and with that of vimentin and of glial fibrillary acidic protein, which identify immature and mature astrocytes, respectively. Our observations show that the two transporters are already expressed at birth in both brain areas with the same cellular localization as in adult rats, as GAT-1 is present in growth cones and terminals only in the cortex, whereas both transporters are expressed in astrocytes in the cortex and thalamus. The distribution of GAT-1 and GAT-3 undergoes postnatal changes reflecting in general the neurogenetic events of the neocortex and thalamus and, more specifically, the maturation of GABAergic innervation. The adult-like pattern of expression is achieved in the third postnatal week in the cortex and in the second postnatal week in the thalamus. The early expression of GAT-1 in GABAergic terminals confirms previous studies showing the existence of neuronal mechanisms of GABA uptake from the embryonic stages. As for the glial localization, the precocious existence of two astrocytic GABA transporters suggests that they operate through different functional mechanisms from birth, whereas their exclusively glial expression in the thalamus indicates that the astroglia plays a major role in the transport, recycling and metabolism of thalamic GABA.

Authors+Show Affiliations

Dipartimento di Fisiologia e Biochimica Generali, Sezione di Istologia e Anatomia Umana, via Celoria 26, 20133 Milan, Italy. laura.vitellaro@unimi.itNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12898208

Citation

Vitellaro-Zuccarello, L, et al. "Expression of GABA Transporters, GAT-1 and GAT-3, in the Cerebral Cortex and Thalamus of the Rat During Postnatal Development." Cell and Tissue Research, vol. 313, no. 3, 2003, pp. 245-57.
Vitellaro-Zuccarello L, Calvaresi N, De Biasi S. Expression of GABA transporters, GAT-1 and GAT-3, in the cerebral cortex and thalamus of the rat during postnatal development. Cell Tissue Res. 2003;313(3):245-57.
Vitellaro-Zuccarello, L., Calvaresi, N., & De Biasi, S. (2003). Expression of GABA transporters, GAT-1 and GAT-3, in the cerebral cortex and thalamus of the rat during postnatal development. Cell and Tissue Research, 313(3), pp. 245-57.
Vitellaro-Zuccarello L, Calvaresi N, De Biasi S. Expression of GABA Transporters, GAT-1 and GAT-3, in the Cerebral Cortex and Thalamus of the Rat During Postnatal Development. Cell Tissue Res. 2003;313(3):245-57. PubMed PMID: 12898208.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of GABA transporters, GAT-1 and GAT-3, in the cerebral cortex and thalamus of the rat during postnatal development. AU - Vitellaro-Zuccarello,L, AU - Calvaresi,N, AU - De Biasi,S, Y1 - 2003/07/24/ PY - 2002/12/04/received PY - 2003/05/09/accepted PY - 2003/8/5/pubmed PY - 2004/6/26/medline PY - 2003/8/5/entrez SP - 245 EP - 57 JF - Cell and tissue research JO - Cell Tissue Res. VL - 313 IS - 3 N2 - The cellular and subcellular localization of two GABA transporters, GAT-1 and GAT-3, was investigated using immunocytochemical methods in the rat cerebral cortex and thalamus during postnatal development. The distribution of the transporters is compared with that of the neuronal marker GABA, and with that of vimentin and of glial fibrillary acidic protein, which identify immature and mature astrocytes, respectively. Our observations show that the two transporters are already expressed at birth in both brain areas with the same cellular localization as in adult rats, as GAT-1 is present in growth cones and terminals only in the cortex, whereas both transporters are expressed in astrocytes in the cortex and thalamus. The distribution of GAT-1 and GAT-3 undergoes postnatal changes reflecting in general the neurogenetic events of the neocortex and thalamus and, more specifically, the maturation of GABAergic innervation. The adult-like pattern of expression is achieved in the third postnatal week in the cortex and in the second postnatal week in the thalamus. The early expression of GAT-1 in GABAergic terminals confirms previous studies showing the existence of neuronal mechanisms of GABA uptake from the embryonic stages. As for the glial localization, the precocious existence of two astrocytic GABA transporters suggests that they operate through different functional mechanisms from birth, whereas their exclusively glial expression in the thalamus indicates that the astroglia plays a major role in the transport, recycling and metabolism of thalamic GABA. SN - 0302-766X UR - https://www.unboundmedicine.com/medline/citation/12898208/Expression_of_GABA_transporters_GAT_1_and_GAT_3_in_the_cerebral_cortex_and_thalamus_of_the_rat_during_postnatal_development_ L2 - https://dx.doi.org/10.1007/s00441-003-0746-9 DB - PRIME DP - Unbound Medicine ER -