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Regulation of the murine renal vitamin D receptor by 1,25-dihydroxyvitamin D3 and calcium.
Proc Natl Acad Sci U S A 2003; 100(17):9733-7PN

Abstract

Renal vitamin D receptor (VDR) is required for 1,25-dihydroxyvitamin D3-[1,25(OH)2D3]-induced renal reabsorption of calcium and for 1,25(OH)2D3-induced 1,25(OH)2D3 24-hydroxylase. The long-term effect of vitamin D and dietary calcium on the expression of renal VDR was examined in the nonobese diabetic mouse. Vitamin D-deficient and vitamin D-replete mice were maintained on diets containing 0.02%, 0.25%, 0.47%, and 1.20% calcium with or without 50 ng of 1,25(OH)2D3 per day. Vitamin D-replete mice on a 1.20% calcium diet had renal VDR levels of 165 fmol/mg protein. Calcium restriction caused renal VDR levels to decrease to <30 fmol/mg protein in vitamin D-deficient mice and to approximately 80 fmol/mg protein in vitamin D-replete mice. When dietary calcium was present, 50 ng of 1,25(OH)2D3 elevated the VDR levels 2- to 10-fold, depending on vitamin D status and the level of calcium. In the absence of either vitamin D or calcium, the VDR mRNA was expressed at a basal level. 1,25(OH)2D3 supplementation caused relative VDR mRNA to increase 8- to 10-fold in the vitamin D-deficient mouse when dietary calcium was available. This increase was completely absent in the calcium-restricted mice. This in vivo study demonstrates that 1,25(OH)2D3 and calcium are both required for renal VDR mRNA expression above a basal level, furthering our understanding of the complex regulation of renal VDR by 1,25(OH)2D3 and calcium.

Authors+Show Affiliations

Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12900504

Citation

Healy, Kevin D., et al. "Regulation of the Murine Renal Vitamin D Receptor By 1,25-dihydroxyvitamin D3 and Calcium." Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 17, 2003, pp. 9733-7.
Healy KD, Zella JB, Prahl JM, et al. Regulation of the murine renal vitamin D receptor by 1,25-dihydroxyvitamin D3 and calcium. Proc Natl Acad Sci USA. 2003;100(17):9733-7.
Healy, K. D., Zella, J. B., Prahl, J. M., & DeLuca, H. F. (2003). Regulation of the murine renal vitamin D receptor by 1,25-dihydroxyvitamin D3 and calcium. Proceedings of the National Academy of Sciences of the United States of America, 100(17), pp. 9733-7.
Healy KD, et al. Regulation of the Murine Renal Vitamin D Receptor By 1,25-dihydroxyvitamin D3 and Calcium. Proc Natl Acad Sci USA. 2003 Aug 19;100(17):9733-7. PubMed PMID: 12900504.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of the murine renal vitamin D receptor by 1,25-dihydroxyvitamin D3 and calcium. AU - Healy,Kevin D, AU - Zella,Julia B, AU - Prahl,Jean M, AU - DeLuca,Hector F, Y1 - 2003/08/04/ PY - 2003/8/6/pubmed PY - 2003/10/30/medline PY - 2003/8/6/entrez SP - 9733 EP - 7 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 100 IS - 17 N2 - Renal vitamin D receptor (VDR) is required for 1,25-dihydroxyvitamin D3-[1,25(OH)2D3]-induced renal reabsorption of calcium and for 1,25(OH)2D3-induced 1,25(OH)2D3 24-hydroxylase. The long-term effect of vitamin D and dietary calcium on the expression of renal VDR was examined in the nonobese diabetic mouse. Vitamin D-deficient and vitamin D-replete mice were maintained on diets containing 0.02%, 0.25%, 0.47%, and 1.20% calcium with or without 50 ng of 1,25(OH)2D3 per day. Vitamin D-replete mice on a 1.20% calcium diet had renal VDR levels of 165 fmol/mg protein. Calcium restriction caused renal VDR levels to decrease to <30 fmol/mg protein in vitamin D-deficient mice and to approximately 80 fmol/mg protein in vitamin D-replete mice. When dietary calcium was present, 50 ng of 1,25(OH)2D3 elevated the VDR levels 2- to 10-fold, depending on vitamin D status and the level of calcium. In the absence of either vitamin D or calcium, the VDR mRNA was expressed at a basal level. 1,25(OH)2D3 supplementation caused relative VDR mRNA to increase 8- to 10-fold in the vitamin D-deficient mouse when dietary calcium was available. This increase was completely absent in the calcium-restricted mice. This in vivo study demonstrates that 1,25(OH)2D3 and calcium are both required for renal VDR mRNA expression above a basal level, furthering our understanding of the complex regulation of renal VDR by 1,25(OH)2D3 and calcium. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/12900504/Regulation_of_the_murine_renal_vitamin_D_receptor_by_125_dihydroxyvitamin_D3_and_calcium_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&amp;pmid=12900504 DB - PRIME DP - Unbound Medicine ER -