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Enantioseparation of warfarin and its metabolites by capillary zone electrophoresis.
Electrophoresis. 2003 Aug; 24(15):2617-26.E

Abstract

A capillary zone electrophoresis (CZE) method with direct ultraviolet (UV)-absorbance detection is presented for the simultaneous enantiomeric separation of warfarin and its main metabolites, including warfarin alcohols, 4'-, 6-, and 7-hydroxywarfarin, using highly sulfated beta-cyclodextrin (HS-beta-CD) as the chiral selector. This chiral separation method was optimized in terms of the electrophoretic parameters, which included the concentration of HS-beta-CD used, the type and composition of organic modifier added to the background electrolyte (BGE) buffer, and the BGE buffer pH. Chiral separation of warfarin and its major metabolites was achieved with high resolution, selectivity, efficiency, repeatability, and reproducibility. This optimized chiral analysis of warfarin along with its metabolites was completed within a satisfactory electrophoresis time of 20 min.

Authors+Show Affiliations

Zhou Q
Department of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore 117543, Republic of Singapore.
Yau WP
No affiliation info available
Chan E
No affiliation info available

MeSH

CyclodextrinsElectrophoresis, CapillaryHydrogen-Ion ConcentrationHydroxylationReproducibility of ResultsSolventsStereoisomerismWarfarinbeta-Cyclodextrins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12900874

Citation

Zhou, Qingyu, et al. "Enantioseparation of Warfarin and Its Metabolites By Capillary Zone Electrophoresis." Electrophoresis, vol. 24, no. 15, 2003, pp. 2617-26.
Zhou Q, Yau WP, Chan E. Enantioseparation of warfarin and its metabolites by capillary zone electrophoresis. Electrophoresis. 2003;24(15):2617-26.
Zhou, Q., Yau, W. P., & Chan, E. (2003). Enantioseparation of warfarin and its metabolites by capillary zone electrophoresis. Electrophoresis, 24(15), 2617-26.
Zhou Q, Yau WP, Chan E. Enantioseparation of Warfarin and Its Metabolites By Capillary Zone Electrophoresis. Electrophoresis. 2003;24(15):2617-26. PubMed PMID: 12900874.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enantioseparation of warfarin and its metabolites by capillary zone electrophoresis. AU - Zhou,Qingyu, AU - Yau,Wai-Ping, AU - Chan,Eli, PY - 2003/8/6/pubmed PY - 2004/6/18/medline PY - 2003/8/6/entrez SP - 2617 EP - 26 JF - Electrophoresis JO - Electrophoresis VL - 24 IS - 15 N2 - A capillary zone electrophoresis (CZE) method with direct ultraviolet (UV)-absorbance detection is presented for the simultaneous enantiomeric separation of warfarin and its main metabolites, including warfarin alcohols, 4'-, 6-, and 7-hydroxywarfarin, using highly sulfated beta-cyclodextrin (HS-beta-CD) as the chiral selector. This chiral separation method was optimized in terms of the electrophoretic parameters, which included the concentration of HS-beta-CD used, the type and composition of organic modifier added to the background electrolyte (BGE) buffer, and the BGE buffer pH. Chiral separation of warfarin and its major metabolites was achieved with high resolution, selectivity, efficiency, repeatability, and reproducibility. This optimized chiral analysis of warfarin along with its metabolites was completed within a satisfactory electrophoresis time of 20 min. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/12900874/Enantioseparation_of_warfarin_and_its_metabolites_by_capillary_zone_electrophoresis_ DB - PRIME DP - Unbound Medicine ER -