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Preparation of insolubilized-DNA film with three-dimensional network and removal of endocrine disruptors.

Abstract

A water-insolubilized film was prepared by UV irradiation on a dried DNA film. When a UV-irradiated DNA was examined using a circular dichroism spectroscopy, a double stranded structure was observed as well as that of native DNA. The UV irradiated DNA film was also accumulated intercalating reagents. These results suggested that the double stranded structure was involved in the UV irradiated DNA film with a three-dimensional network. The thymine-thymine dimer formation was suggested to be involved in the cross-linking reactions by the polymerization analysis using poly(dA)-poly(dT) and poly(dG)-poly(dC). We also demonstrate the utilization of the UV-irradiated DNA film as a functional material for the accumulation of harmful DNA intercalating pollutants in aqueous solution. These results suggested that the UV-irradiated DNA film was applicable as a functional material for medical, engineering and environmental sciences.

Authors+Show Affiliations

Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12903365

Citation

Yamada, M, et al. "Preparation of insolubilized-DNA Film With Three-dimensional Network and Removal of Endocrine Disruptors." Nucleic Acids Symposium Series, 2000, pp. 255-6.
Yamada M, Kato K, Nomizu M, et al. Preparation of insolubilized-DNA film with three-dimensional network and removal of endocrine disruptors. Nucleic Acids Symp Ser. 2000.
Yamada, M., Kato, K., Nomizu, M., Sakairi, N., Ohkawa, K., Yamamoto, H., & Nishi, N. (2000). Preparation of insolubilized-DNA film with three-dimensional network and removal of endocrine disruptors. Nucleic Acids Symposium Series, (44), 255-6.
Yamada M, et al. Preparation of insolubilized-DNA Film With Three-dimensional Network and Removal of Endocrine Disruptors. Nucleic Acids Symp Ser. 2000;(44)255-6. PubMed PMID: 12903365.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation of insolubilized-DNA film with three-dimensional network and removal of endocrine disruptors. AU - Yamada,M, AU - Kato,K, AU - Nomizu,M, AU - Sakairi,N, AU - Ohkawa,K, AU - Yamamoto,H, AU - Nishi,N, PY - 2003/8/9/pubmed PY - 2003/10/3/medline PY - 2003/8/9/entrez SP - 255 EP - 6 JF - Nucleic acids symposium series JO - Nucleic Acids Symp. Ser. IS - 44 N2 - A water-insolubilized film was prepared by UV irradiation on a dried DNA film. When a UV-irradiated DNA was examined using a circular dichroism spectroscopy, a double stranded structure was observed as well as that of native DNA. The UV irradiated DNA film was also accumulated intercalating reagents. These results suggested that the double stranded structure was involved in the UV irradiated DNA film with a three-dimensional network. The thymine-thymine dimer formation was suggested to be involved in the cross-linking reactions by the polymerization analysis using poly(dA)-poly(dT) and poly(dG)-poly(dC). We also demonstrate the utilization of the UV-irradiated DNA film as a functional material for the accumulation of harmful DNA intercalating pollutants in aqueous solution. These results suggested that the UV-irradiated DNA film was applicable as a functional material for medical, engineering and environmental sciences. SN - 0261-3166 UR - https://www.unboundmedicine.com/medline/citation/12903365/Preparation_of_insolubilized_DNA_film_with_three_dimensional_network_and_removal_of_endocrine_disruptors_ DB - PRIME DP - Unbound Medicine ER -