Tags

Type your tag names separated by a space and hit enter

Spontaneous synaptic activity is primarily GABAergic in vestibular nucleus neurons of the chick embryo.
J Neurophysiol 2003; 90(2):1182-92JN

Abstract

The principal cells of the chick tangential nucleus are vestibular nucleus neurons participating in the vestibular reflexes. In 16-day embryos, the application of glutamate receptor antagonists abolished the postsynaptic responses generated on vestibular-nerve stimulation, but spontaneous synaptic activity was largely unaffected. Here, spontaneous synaptic activity was characterized in principal cells from brain slices at E16 using whole cell voltage-clamp recordings. With KCl electrodes, the frequency of spontaneous inward currents was 3.1 Hz at -60 mV, and the reversal potential was +4 mV. Cs-gluconate pipette solution allowed the discrimination of glycine/GABA(A) versus glutamate receptor-mediated events according to their different reversal potentials. The ratio for spontaneous excitatory to inhibitory events was about 1:4. Seventy-four percent of the outward events were GABA(A), whereas 26% were glycine receptor-mediated events. Both pre- and postsynaptic GABA(B) receptor effects were shown, with presynaptic GABA(B) receptors inhibiting 40% of spontaneous excitatory postsynaptic currents (sEPSCs) and 53% of spontaneous inhibitory postsynaptic currents (sIPSCs). With TTX, the frequency decreased approximately 50% for EPSCs and 23% for IPSCs. These data indicate that the spontaneous synaptic activity recorded in the principal cells at E16 is primarily inhibitory, action potential-independent, and based on the activation of GABA(A) receptors that can be modulated by presynaptic GABA(B) receptors.

Authors+Show Affiliations

Department of Anatomy and Cell Biology and Neuroscience Program, George Washington University Medical Center, Washington DC 20037, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12904504

Citation

Shao, Mei, et al. "Spontaneous Synaptic Activity Is Primarily GABAergic in Vestibular Nucleus Neurons of the Chick Embryo." Journal of Neurophysiology, vol. 90, no. 2, 2003, pp. 1182-92.
Shao M, Hirsch JC, Giaume C, et al. Spontaneous synaptic activity is primarily GABAergic in vestibular nucleus neurons of the chick embryo. J Neurophysiol. 2003;90(2):1182-92.
Shao, M., Hirsch, J. C., Giaume, C., & Peusner, K. D. (2003). Spontaneous synaptic activity is primarily GABAergic in vestibular nucleus neurons of the chick embryo. Journal of Neurophysiology, 90(2), pp. 1182-92.
Shao M, et al. Spontaneous Synaptic Activity Is Primarily GABAergic in Vestibular Nucleus Neurons of the Chick Embryo. J Neurophysiol. 2003;90(2):1182-92. PubMed PMID: 12904504.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spontaneous synaptic activity is primarily GABAergic in vestibular nucleus neurons of the chick embryo. AU - Shao,Mei, AU - Hirsch,June C, AU - Giaume,Christian, AU - Peusner,Kenna D, PY - 2003/8/9/pubmed PY - 2003/10/2/medline PY - 2003/8/9/entrez SP - 1182 EP - 92 JF - Journal of neurophysiology JO - J. Neurophysiol. VL - 90 IS - 2 N2 - The principal cells of the chick tangential nucleus are vestibular nucleus neurons participating in the vestibular reflexes. In 16-day embryos, the application of glutamate receptor antagonists abolished the postsynaptic responses generated on vestibular-nerve stimulation, but spontaneous synaptic activity was largely unaffected. Here, spontaneous synaptic activity was characterized in principal cells from brain slices at E16 using whole cell voltage-clamp recordings. With KCl electrodes, the frequency of spontaneous inward currents was 3.1 Hz at -60 mV, and the reversal potential was +4 mV. Cs-gluconate pipette solution allowed the discrimination of glycine/GABA(A) versus glutamate receptor-mediated events according to their different reversal potentials. The ratio for spontaneous excitatory to inhibitory events was about 1:4. Seventy-four percent of the outward events were GABA(A), whereas 26% were glycine receptor-mediated events. Both pre- and postsynaptic GABA(B) receptor effects were shown, with presynaptic GABA(B) receptors inhibiting 40% of spontaneous excitatory postsynaptic currents (sEPSCs) and 53% of spontaneous inhibitory postsynaptic currents (sIPSCs). With TTX, the frequency decreased approximately 50% for EPSCs and 23% for IPSCs. These data indicate that the spontaneous synaptic activity recorded in the principal cells at E16 is primarily inhibitory, action potential-independent, and based on the activation of GABA(A) receptors that can be modulated by presynaptic GABA(B) receptors. SN - 0022-3077 UR - https://www.unboundmedicine.com/medline/citation/12904504/Spontaneous_synaptic_activity_is_primarily_GABAergic_in_vestibular_nucleus_neurons_of_the_chick_embryo_ L2 - http://www.physiology.org/doi/full/10.1152/jn.00076.2003?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -