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[Fine mapping of susceptibility genes loci within chromosome 1 in Chinese Han families with type 2 diabetes].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002 Jun; 24(3):234-7.ZY

Abstract

OBJECTIVES

To confirm previous whole-genome scan results of mapping type 2 diabetes susceptibility genes in chromosome 1 in Northern Chinese Han population by conducting a new genome scan with both an enlarged number of type 2 diabetes families and a new set of microsatellite markers.

METHODS

A genome scan method was applied. After multiplexed PCR, electrophoreses, genescan and genotyping analysis, size informations for all loci were obtained, and a further study was done using both parametric and non-parametric linkage analysis to calculate the P-values and Z-values of these loci.

RESULTS

A total of 34 microsatellite markers distributed within 5 regions along chromosome 1 were surveyed, and 12,000 genotypes were screened. Evidence of linkage with diabetes was identified for 8 of the 34 loci (all the P-values of the 8 loci distributed in 3 regions were lower than 0.05, and the highest Z-value was 2.17). Interestingly, all the 5 markers at the P terminal 1p36.3-1p36.23 region, spanning a long range of 16.9 cM, suggested to be linked with the disease. The results of the other two regions were not consistent with the previous ones.

CONCLUSIONS

The study results have confirmed those gained in the previous genome-wide scan. The fact that all 5 loci at the P terminal region displayed linkage with diabetes suggests that more than 1 susceptibility gene may reside in this region.

Authors+Show Affiliations

National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12905624

Citation

Du, Wei-nan, et al. "[Fine Mapping of Susceptibility Genes Loci Within Chromosome 1 in Chinese Han Families With Type 2 Diabetes]." Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, vol. 24, no. 3, 2002, pp. 234-7.
Du WN, Sun HX, Wang H, et al. [Fine mapping of susceptibility genes loci within chromosome 1 in Chinese Han families with type 2 diabetes]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002;24(3):234-7.
Du, W. N., Sun, H. X., Wang, H., Qiang, B. Q., Yao, Z. J., Gu, J., Xiong, M. M., Huang, W., Chen, Z., Zuo, J., Hua, X. F., Gao, W., Sun, Q., & Fang, F. D. (2002). [Fine mapping of susceptibility genes loci within chromosome 1 in Chinese Han families with type 2 diabetes]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, 24(3), 234-7.
Du WN, et al. [Fine Mapping of Susceptibility Genes Loci Within Chromosome 1 in Chinese Han Families With Type 2 Diabetes]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002;24(3):234-7. PubMed PMID: 12905624.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Fine mapping of susceptibility genes loci within chromosome 1 in Chinese Han families with type 2 diabetes]. AU - Du,Wei-nan, AU - Sun,Hong-xia, AU - Wang,Heng, AU - Qiang,Bo-qin, AU - Yao,Zhi-jian, AU - Gu,Jun, AU - Xiong,Mo-miao, AU - Huang,Wei, AU - Chen,Zhu, AU - Zuo,Jin, AU - Hua,Xiu-feng, AU - Gao,Wei, AU - Sun,Qi, AU - Fang,Fu-de, PY - 2003/8/9/pubmed PY - 2004/4/7/medline PY - 2003/8/9/entrez SP - 234 EP - 7 JF - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae JO - Zhongguo Yi Xue Ke Xue Yuan Xue Bao VL - 24 IS - 3 N2 - OBJECTIVES: To confirm previous whole-genome scan results of mapping type 2 diabetes susceptibility genes in chromosome 1 in Northern Chinese Han population by conducting a new genome scan with both an enlarged number of type 2 diabetes families and a new set of microsatellite markers. METHODS: A genome scan method was applied. After multiplexed PCR, electrophoreses, genescan and genotyping analysis, size informations for all loci were obtained, and a further study was done using both parametric and non-parametric linkage analysis to calculate the P-values and Z-values of these loci. RESULTS: A total of 34 microsatellite markers distributed within 5 regions along chromosome 1 were surveyed, and 12,000 genotypes were screened. Evidence of linkage with diabetes was identified for 8 of the 34 loci (all the P-values of the 8 loci distributed in 3 regions were lower than 0.05, and the highest Z-value was 2.17). Interestingly, all the 5 markers at the P terminal 1p36.3-1p36.23 region, spanning a long range of 16.9 cM, suggested to be linked with the disease. The results of the other two regions were not consistent with the previous ones. CONCLUSIONS: The study results have confirmed those gained in the previous genome-wide scan. The fact that all 5 loci at the P terminal region displayed linkage with diabetes suggests that more than 1 susceptibility gene may reside in this region. SN - 1000-503X UR - https://www.unboundmedicine.com/medline/citation/12905624/[Fine_mapping_of_susceptibility_genes_loci_within_chromosome_1_in_Chinese_Han_families_with_type_2_diabetes]_ L2 - http://www.diseaseinfosearch.org/result/2243 DB - PRIME DP - Unbound Medicine ER -