Tags

Type your tag names separated by a space and hit enter

Validated method for the determination of six metabolites derived from artichoke leaf extract in human plasma by high-performance liquid chromatography-coulometric-array detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Aug 15; 793(2):367-75.JC

Abstract

A validated method was developed for the simultaneous determination of the hydroxycinnamates caffeic (CA), dihydrocaffeic (DHCA), ferulic (FA), dihydroferulic (DHFA), and isoferulic acid (IFA) and the flavonoid luteolin (LUT) in human plasma as metabolites derived from artichoke leaf extract. The method involves sample preparation followed by separation using high-performance liquid chromatography on reversed-phase material with a polar endcapping (Aqua-C(18), 250 x 4.6 mm). Selectivity and sensitivity towards the target compounds were achieved by electrochemical array detection (CoulArray). Calibration curves were constructed in the ranges 2.1-51.7 ng x mL(-1) (CA), 2.0-76.7 ng mL(-1) (DHCA), 2.2-53.7 ng x mL(-1) (FA), 2.1-79.2 ng x mL(-1) (DHFA), 1.1-52.6 ng x mL(-1) (IFA) and 2.1-258.6 ng x mL(-1) (LUT). Linearity could be shown for all target compounds over the entire calibration range. Values for within-day and between-day precision and accuracy were in accordance with the international guidelines for validation of bioanalytical methods. It is concluded that this newly developed method is appropriate for analysing samples from bioavailability and pharmacokinetic studies after oral administration of artichoke leaf extract.

Authors+Show Affiliations

Lichtwer Pharma AG, Wallenroder Strasse 8-10, 13485 Berlin, Germany.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

12906912

Citation

Wittemer, Sabine M., and Markus Veit. "Validated Method for the Determination of Six Metabolites Derived From Artichoke Leaf Extract in Human Plasma By High-performance Liquid Chromatography-coulometric-array Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 793, no. 2, 2003, pp. 367-75.
Wittemer SM, Veit M. Validated method for the determination of six metabolites derived from artichoke leaf extract in human plasma by high-performance liquid chromatography-coulometric-array detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2003;793(2):367-75.
Wittemer, S. M., & Veit, M. (2003). Validated method for the determination of six metabolites derived from artichoke leaf extract in human plasma by high-performance liquid chromatography-coulometric-array detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 793(2), 367-75.
Wittemer SM, Veit M. Validated Method for the Determination of Six Metabolites Derived From Artichoke Leaf Extract in Human Plasma By High-performance Liquid Chromatography-coulometric-array Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Aug 15;793(2):367-75. PubMed PMID: 12906912.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validated method for the determination of six metabolites derived from artichoke leaf extract in human plasma by high-performance liquid chromatography-coulometric-array detection. AU - Wittemer,Sabine M, AU - Veit,Markus, PY - 2003/8/9/pubmed PY - 2004/7/9/medline PY - 2003/8/9/entrez SP - 367 EP - 75 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 793 IS - 2 N2 - A validated method was developed for the simultaneous determination of the hydroxycinnamates caffeic (CA), dihydrocaffeic (DHCA), ferulic (FA), dihydroferulic (DHFA), and isoferulic acid (IFA) and the flavonoid luteolin (LUT) in human plasma as metabolites derived from artichoke leaf extract. The method involves sample preparation followed by separation using high-performance liquid chromatography on reversed-phase material with a polar endcapping (Aqua-C(18), 250 x 4.6 mm). Selectivity and sensitivity towards the target compounds were achieved by electrochemical array detection (CoulArray). Calibration curves were constructed in the ranges 2.1-51.7 ng x mL(-1) (CA), 2.0-76.7 ng mL(-1) (DHCA), 2.2-53.7 ng x mL(-1) (FA), 2.1-79.2 ng x mL(-1) (DHFA), 1.1-52.6 ng x mL(-1) (IFA) and 2.1-258.6 ng x mL(-1) (LUT). Linearity could be shown for all target compounds over the entire calibration range. Values for within-day and between-day precision and accuracy were in accordance with the international guidelines for validation of bioanalytical methods. It is concluded that this newly developed method is appropriate for analysing samples from bioavailability and pharmacokinetic studies after oral administration of artichoke leaf extract. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/12906912/Validated_method_for_the_determination_of_six_metabolites_derived_from_artichoke_leaf_extract_in_human_plasma_by_high_performance_liquid_chromatography_coulometric_array_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570023203003520 DB - PRIME DP - Unbound Medicine ER -