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Effect of altered dietary n-3 fatty acid intake upon plasma lipid fatty acid composition, conversion of [13C]alpha-linolenic acid to longer-chain fatty acids and partitioning towards beta-oxidation in older men.
Br J Nutr. 2003 Aug; 90(2):311-21.BJ

Abstract

The effect of increased dietary intakes of alpha-linolenic acid (ALNA) or eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) for 2 months upon plasma lipid composition and capacity for conversion of ALNA to longer-chain metabolites was investigated in healthy men (52 (SD 12) years). After a 4-week baseline period when the subjects substituted a control spread, a test meal containing [U-(13)C]ALNA (700 mg) was consumed to measure conversion to EPA, docosapentaenoic acid (DPA) and DHA over 48 h. Subjects were then randomised to one of three groups for 8 weeks before repeating the tracer study: (1) continued on same intake (control, n 5); (2) increased ALNA intake (10 g/d, n 4); (3) increased EPA+DHA intake (1.5 g/d, n 5). At baseline, apparent fractional conversion of labelled ALNA was: EPA 2.80, DPA 1.20 and DHA 0.04 %. After 8 weeks on the control diet, plasma lipid composition and [(13)C]ALNA conversion remained unchanged compared with baseline. The high-ALNA diet resulted in raised plasma triacylglycerol-EPA and -DPA concentrations and phosphatidylcholine-EPA concentration, whilst [(13)C]ALNA conversion was similar to baseline. The high-(EPA+DHA) diet raised plasma phosphatidylcholine-EPA and -DHA concentrations, decreased [(13)C]ALNA conversion to EPA (2-fold) and DPA (4-fold), whilst [(13)C]ALNA conversion to DHA was unchanged. The dietary interventions did not alter partitioning of ALNA towards beta-oxidation. The present results indicate ALNA conversion was down-regulated by increased product (EPA+DHA) availability, but was not up-regulated by increased substrate (ALNA) consumption. This suggests regulation of ALNA conversion may limit the influence of variations in dietary n-3 fatty acid intake on plasma lipid compositions.

Authors+Show Affiliations

Institute of Human Nutrition, University of Southampton, UK. g.c.burdge@soton.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12908891

Citation

Burdge, Graham C., et al. "Effect of Altered Dietary N-3 Fatty Acid Intake Upon Plasma Lipid Fatty Acid Composition, Conversion of [13C]alpha-linolenic Acid to Longer-chain Fatty Acids and Partitioning Towards Beta-oxidation in Older Men." The British Journal of Nutrition, vol. 90, no. 2, 2003, pp. 311-21.
Burdge GC, Finnegan YE, Minihane AM, et al. Effect of altered dietary n-3 fatty acid intake upon plasma lipid fatty acid composition, conversion of [13C]alpha-linolenic acid to longer-chain fatty acids and partitioning towards beta-oxidation in older men. Br J Nutr. 2003;90(2):311-21.
Burdge, G. C., Finnegan, Y. E., Minihane, A. M., Williams, C. M., & Wootton, S. A. (2003). Effect of altered dietary n-3 fatty acid intake upon plasma lipid fatty acid composition, conversion of [13C]alpha-linolenic acid to longer-chain fatty acids and partitioning towards beta-oxidation in older men. The British Journal of Nutrition, 90(2), 311-21.
Burdge GC, et al. Effect of Altered Dietary N-3 Fatty Acid Intake Upon Plasma Lipid Fatty Acid Composition, Conversion of [13C]alpha-linolenic Acid to Longer-chain Fatty Acids and Partitioning Towards Beta-oxidation in Older Men. Br J Nutr. 2003;90(2):311-21. PubMed PMID: 12908891.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of altered dietary n-3 fatty acid intake upon plasma lipid fatty acid composition, conversion of [13C]alpha-linolenic acid to longer-chain fatty acids and partitioning towards beta-oxidation in older men. AU - Burdge,Graham C, AU - Finnegan,Yvonne E, AU - Minihane,Anne M, AU - Williams,Christine M, AU - Wootton,Stephen A, PY - 2003/8/12/pubmed PY - 2003/9/16/medline PY - 2003/8/12/entrez SP - 311 EP - 21 JF - The British journal of nutrition JO - Br J Nutr VL - 90 IS - 2 N2 - The effect of increased dietary intakes of alpha-linolenic acid (ALNA) or eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) for 2 months upon plasma lipid composition and capacity for conversion of ALNA to longer-chain metabolites was investigated in healthy men (52 (SD 12) years). After a 4-week baseline period when the subjects substituted a control spread, a test meal containing [U-(13)C]ALNA (700 mg) was consumed to measure conversion to EPA, docosapentaenoic acid (DPA) and DHA over 48 h. Subjects were then randomised to one of three groups for 8 weeks before repeating the tracer study: (1) continued on same intake (control, n 5); (2) increased ALNA intake (10 g/d, n 4); (3) increased EPA+DHA intake (1.5 g/d, n 5). At baseline, apparent fractional conversion of labelled ALNA was: EPA 2.80, DPA 1.20 and DHA 0.04 %. After 8 weeks on the control diet, plasma lipid composition and [(13)C]ALNA conversion remained unchanged compared with baseline. The high-ALNA diet resulted in raised plasma triacylglycerol-EPA and -DPA concentrations and phosphatidylcholine-EPA concentration, whilst [(13)C]ALNA conversion was similar to baseline. The high-(EPA+DHA) diet raised plasma phosphatidylcholine-EPA and -DHA concentrations, decreased [(13)C]ALNA conversion to EPA (2-fold) and DPA (4-fold), whilst [(13)C]ALNA conversion to DHA was unchanged. The dietary interventions did not alter partitioning of ALNA towards beta-oxidation. The present results indicate ALNA conversion was down-regulated by increased product (EPA+DHA) availability, but was not up-regulated by increased substrate (ALNA) consumption. This suggests regulation of ALNA conversion may limit the influence of variations in dietary n-3 fatty acid intake on plasma lipid compositions. SN - 0007-1145 UR - https://www.unboundmedicine.com/medline/citation/12908891/Effect_of_altered_dietary_n_3_fatty_acid_intake_upon_plasma_lipid_fatty_acid_composition_conversion_of_[13C]alpha_linolenic_acid_to_longer_chain_fatty_acids_and_partitioning_towards_beta_oxidation_in_older_men_ L2 - https://www.cambridge.org/core/product/identifier/S0007114503001302/type/journal_article DB - PRIME DP - Unbound Medicine ER -