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Comparison and evaluation of DRI methamphetamine, DRI ecstasy, Abuscreen ONLINE amphetamine, and a modified Abuscreen ONLINE amphetamine screening immunoassays for the detection of amphetamine (AMP), methamphetamine (MTH), 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxymethamphetamine (MDMA) in human urine.
J Anal Toxicol 2003 Jul-Aug; 27(5):265-9JA

Abstract

The performances of four immunoassays (DRI amphetamines, DRI ecstasy, Abuscreen ONLINE amphetamines, and a modified Abuscreen ONLINE amphetamines) were evaluated for control failure rates, sensitivity, and specificity for amphetamine (AMP), methamphetamine (MTH), 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxymethamphetamine (MDMA). The two DRI reagents and the ONLINE reagents were run according to manufacturer specifications using a Roche Hitachi Modular DDP system. The modified ONLINE reagent was calibrated with MDMA and had 16mM sodium periodate added to the R2 reagent. These assays were run on approximately 27,500 human urine samples and 7000 control urine samples prepared at 350 and 674 ng/mL over the course of 8 days. All assays were calibrated using a single point, qualitative cutoff standard with the manufacturer-recommended compound at the Department of Defense cutoff (500 ng/mL). Gas chromatography-mass spectrometry (GC-MS) confirmation was conducted on screened-positive samples. Control performance for the manufacturer recommended assays was excellent, with a maximum qualitative control failure rate of 2.03%. The modified ONLINE reagent demonstrated poor control performance with a maximum failure rate of 38.3% and showed no improved MDMA sensitivity when compared with the ONLINE reagent; the confirmation rate (20%) was improved when compared with the production ONLINE reagent (8%). The DRI ecstasy reagent provided improved sensitivity for MDMA as compared with the ONLINE reagent, with approximately 23% more samples screening and confirming positive for MDMA and a confirmation rate of approximately 90%. The DRI methamphetamine reagent had a low confirmation rate (6% or less) and produced numerous positives for samples with only ephedrine or pseudoephedrine present.

Authors+Show Affiliations

Aegis Sciences Corp., 345 Hill Avenue, Nashville, Tennessee 37210, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12908938

Citation

Stout, Peter R., et al. "Comparison and Evaluation of DRI Methamphetamine, DRI Ecstasy, Abuscreen ONLINE Amphetamine, and a Modified Abuscreen ONLINE Amphetamine Screening Immunoassays for the Detection of Amphetamine (AMP), Methamphetamine (MTH), 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxymethamphetamine (MDMA) in Human Urine." Journal of Analytical Toxicology, vol. 27, no. 5, 2003, pp. 265-9.
Stout PR, Klette KL, Wiegand R. Comparison and evaluation of DRI methamphetamine, DRI ecstasy, Abuscreen ONLINE amphetamine, and a modified Abuscreen ONLINE amphetamine screening immunoassays for the detection of amphetamine (AMP), methamphetamine (MTH), 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxymethamphetamine (MDMA) in human urine. J Anal Toxicol. 2003;27(5):265-9.
Stout, P. R., Klette, K. L., & Wiegand, R. (2003). Comparison and evaluation of DRI methamphetamine, DRI ecstasy, Abuscreen ONLINE amphetamine, and a modified Abuscreen ONLINE amphetamine screening immunoassays for the detection of amphetamine (AMP), methamphetamine (MTH), 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxymethamphetamine (MDMA) in human urine. Journal of Analytical Toxicology, 27(5), pp. 265-9.
Stout PR, Klette KL, Wiegand R. Comparison and Evaluation of DRI Methamphetamine, DRI Ecstasy, Abuscreen ONLINE Amphetamine, and a Modified Abuscreen ONLINE Amphetamine Screening Immunoassays for the Detection of Amphetamine (AMP), Methamphetamine (MTH), 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxymethamphetamine (MDMA) in Human Urine. J Anal Toxicol. 2003;27(5):265-9. PubMed PMID: 12908938.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison and evaluation of DRI methamphetamine, DRI ecstasy, Abuscreen ONLINE amphetamine, and a modified Abuscreen ONLINE amphetamine screening immunoassays for the detection of amphetamine (AMP), methamphetamine (MTH), 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxymethamphetamine (MDMA) in human urine. AU - Stout,Peter R, AU - Klette,Kevin L, AU - Wiegand,Russell, PY - 2003/8/12/pubmed PY - 2004/7/3/medline PY - 2003/8/12/entrez SP - 265 EP - 9 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 27 IS - 5 N2 - The performances of four immunoassays (DRI amphetamines, DRI ecstasy, Abuscreen ONLINE amphetamines, and a modified Abuscreen ONLINE amphetamines) were evaluated for control failure rates, sensitivity, and specificity for amphetamine (AMP), methamphetamine (MTH), 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxymethamphetamine (MDMA). The two DRI reagents and the ONLINE reagents were run according to manufacturer specifications using a Roche Hitachi Modular DDP system. The modified ONLINE reagent was calibrated with MDMA and had 16mM sodium periodate added to the R2 reagent. These assays were run on approximately 27,500 human urine samples and 7000 control urine samples prepared at 350 and 674 ng/mL over the course of 8 days. All assays were calibrated using a single point, qualitative cutoff standard with the manufacturer-recommended compound at the Department of Defense cutoff (500 ng/mL). Gas chromatography-mass spectrometry (GC-MS) confirmation was conducted on screened-positive samples. Control performance for the manufacturer recommended assays was excellent, with a maximum qualitative control failure rate of 2.03%. The modified ONLINE reagent demonstrated poor control performance with a maximum failure rate of 38.3% and showed no improved MDMA sensitivity when compared with the ONLINE reagent; the confirmation rate (20%) was improved when compared with the production ONLINE reagent (8%). The DRI ecstasy reagent provided improved sensitivity for MDMA as compared with the ONLINE reagent, with approximately 23% more samples screening and confirming positive for MDMA and a confirmation rate of approximately 90%. The DRI methamphetamine reagent had a low confirmation rate (6% or less) and produced numerous positives for samples with only ephedrine or pseudoephedrine present. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/12908938/Comparison_and_evaluation_of_DRI_methamphetamine_DRI_ecstasy_Abuscreen_ONLINE_amphetamine_and_a_modified_Abuscreen_ONLINE_amphetamine_screening_immunoassays_for_the_detection_of_amphetamine__AMP__methamphetamine__MTH__34_methylenedioxyamphetamine__MDA__and_34_methylenedioxymethamphetamine__MDMA__in_human_urine_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/27.5.265 DB - PRIME DP - Unbound Medicine ER -