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Quantitation of cocaine and its major metabolites in human saliva using gas chromatography-positive chemical ionization-mass spectrometry (GC-PCI-MS).
J Anal Toxicol. 2003 Jul-Aug; 27(5):270-4.JA

Abstract

An analytical method for the simultaneous determination of cocaine (COC) and its major metabolites, ecgonine methyl ester (EME) and benzoylecgonine (BEG), in saliva was developed. The method involves liquid-liquid extraction in Toxitubes A, derivatization with 99:1 (v/v) N,O-bis-trimethylsilyltrifluoroacetamide (BSTFA)/trimethylchlorosilane (TMCS) and gas chromatography-mass spectrometry (GC-MS) determination. The detector response thus obtained is linear over the range 25-1000 ng/mL, with a reproducibility better than 93% and a recovery close to 100% for the three analytes. The limits of detection achieved were 0.9 ng/mL for EME, 2.2 ng/mL for cocaine, and 0.2 ng/mL for BEG, and the limits of quantitation were 3.0 ng/mL for EME, 7.4 ng/mL for cocaine, and 0.8 ng/mL for BEG. The proposed method was applied to 48 saliva samples from cocaine users, 96% of which were positive for the drug and/or its metabolites. Saliva is thus a suitable biological fluid for determining cocaine, EME, and BEG by GC-PCI-MS.

Authors+Show Affiliations

Institute of Legal Medicine, Forensic Toxicology Service, Faculty of Medicine, University of Santiago de Compostela, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12908939

Citation

Cámpora, P, et al. "Quantitation of Cocaine and Its Major Metabolites in Human Saliva Using Gas Chromatography-positive Chemical Ionization-mass Spectrometry (GC-PCI-MS)." Journal of Analytical Toxicology, vol. 27, no. 5, 2003, pp. 270-4.
Cámpora P, Bermejo AM, Tabernero MJ, et al. Quantitation of cocaine and its major metabolites in human saliva using gas chromatography-positive chemical ionization-mass spectrometry (GC-PCI-MS). J Anal Toxicol. 2003;27(5):270-4.
Cámpora, P., Bermejo, A. M., Tabernero, M. J., & Fernández, P. (2003). Quantitation of cocaine and its major metabolites in human saliva using gas chromatography-positive chemical ionization-mass spectrometry (GC-PCI-MS). Journal of Analytical Toxicology, 27(5), 270-4.
Cámpora P, et al. Quantitation of Cocaine and Its Major Metabolites in Human Saliva Using Gas Chromatography-positive Chemical Ionization-mass Spectrometry (GC-PCI-MS). J Anal Toxicol. 2003 Jul-Aug;27(5):270-4. PubMed PMID: 12908939.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitation of cocaine and its major metabolites in human saliva using gas chromatography-positive chemical ionization-mass spectrometry (GC-PCI-MS). AU - Cámpora,P, AU - Bermejo,A M, AU - Tabernero,M J, AU - Fernández,P, PY - 2003/8/12/pubmed PY - 2004/7/3/medline PY - 2003/8/12/entrez SP - 270 EP - 4 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 27 IS - 5 N2 - An analytical method for the simultaneous determination of cocaine (COC) and its major metabolites, ecgonine methyl ester (EME) and benzoylecgonine (BEG), in saliva was developed. The method involves liquid-liquid extraction in Toxitubes A, derivatization with 99:1 (v/v) N,O-bis-trimethylsilyltrifluoroacetamide (BSTFA)/trimethylchlorosilane (TMCS) and gas chromatography-mass spectrometry (GC-MS) determination. The detector response thus obtained is linear over the range 25-1000 ng/mL, with a reproducibility better than 93% and a recovery close to 100% for the three analytes. The limits of detection achieved were 0.9 ng/mL for EME, 2.2 ng/mL for cocaine, and 0.2 ng/mL for BEG, and the limits of quantitation were 3.0 ng/mL for EME, 7.4 ng/mL for cocaine, and 0.8 ng/mL for BEG. The proposed method was applied to 48 saliva samples from cocaine users, 96% of which were positive for the drug and/or its metabolites. Saliva is thus a suitable biological fluid for determining cocaine, EME, and BEG by GC-PCI-MS. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/12908939/Quantitation_of_cocaine_and_its_major_metabolites_in_human_saliva_using_gas_chromatography_positive_chemical_ionization_mass_spectrometry__GC_PCI_MS__ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/27.5.270 DB - PRIME DP - Unbound Medicine ER -