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Tape stripping and sodium dodecyl sulfate treatment increase the molecular weight cutoff of polyethylene glycol penetration across murine skin.
Arch Dermatol Res. 2003 Aug; 295(4):169-74.AD

Abstract

Prior studies in hairless mice have demonstrated that acute barrier disruption by acetone treatment increases the molecular weight (MW) cutoff of polyethylene glycol (PEG) penetration through the skin. The objective of the present study was to further investigate the dependence of permeability on MW with different forms of barrier disruption. A series of PEGs ranging in MW from near 300 to over 1000 Da were used to study the effects of tape stripping and sodium dodecyl sulfate (SDS) treatment on the MW permeability profiles of mouse skin in vitro. The 12-h percutaneous penetration of all the PEG 300, 600, and 1000 oligomers generally increased as a function of transepidermal water loss (TEWL) of the skin, either tape-stripped or SDS-treated. In addition, the total penetration of PEG oligomers across control skin, and skin tape-stripped and SDS-treated to different degrees of barrier disruption progressively decreased with increasing MW. There were no significant differences in the percutaneous penetration of the PEG oligomers between skin tape-stripped and SDS-treated to the same degree of barrier disruption. The penetration enhancement relative to control skin was more prominent with larger molecules. The MW cutoff for skin penetration increased with the degree of barrier disruption irrespective of the treatment applied, and was 986 Da (tape stripping) and 766 Da (SDS treatment) at TEWL levels in the range 10-20 g/m(2) per h in comparison with 414 Da for control skin. In accordance with previous findings in acetone-treated mouse skin, the results strongly suggest that, irrespective of the form of barrier disruption applied, not only higher amounts but also more varieties of chemicals (larger molecules) may penetrate skin with a compromised barrier than normal skin.

Authors+Show Affiliations

Tsai JC
Institute of Clinical Pharmacy, College of Medicine, National Cheng Kung University, 1 University Road, 70101 Tainan, Taiwan. jctsai@mail.ncku.edu.tw
Shen LC
No affiliation info available
Sheu HM
No affiliation info available
Lu CC
No affiliation info available

MeSH

Administration, CutaneousAnimalsEpidermal CellsEpidermisFemaleMiceMice, HairlessMolecular WeightPermeabilityPolyethylene GlycolsSkin AbsorptionSodium Dodecyl SulfateSurface-Active AgentsTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12910356

Citation

Tsai, J-C, et al. "Tape Stripping and Sodium Dodecyl Sulfate Treatment Increase the Molecular Weight Cutoff of Polyethylene Glycol Penetration Across Murine Skin." Archives of Dermatological Research, vol. 295, no. 4, 2003, pp. 169-74.
Tsai JC, Shen LC, Sheu HM, et al. Tape stripping and sodium dodecyl sulfate treatment increase the molecular weight cutoff of polyethylene glycol penetration across murine skin. Arch Dermatol Res. 2003;295(4):169-74.
Tsai, J. C., Shen, L. C., Sheu, H. M., & Lu, C. C. (2003). Tape stripping and sodium dodecyl sulfate treatment increase the molecular weight cutoff of polyethylene glycol penetration across murine skin. Archives of Dermatological Research, 295(4), 169-74.
Tsai JC, et al. Tape Stripping and Sodium Dodecyl Sulfate Treatment Increase the Molecular Weight Cutoff of Polyethylene Glycol Penetration Across Murine Skin. Arch Dermatol Res. 2003;295(4):169-74. PubMed PMID: 12910356.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tape stripping and sodium dodecyl sulfate treatment increase the molecular weight cutoff of polyethylene glycol penetration across murine skin. AU - Tsai,J-C, AU - Shen,L-C, AU - Sheu,H-M, AU - Lu,C-C, Y1 - 2003/07/01/ PY - 2002/11/20/received PY - 2002/02/05/revised PY - 2003/06/04/accepted PY - 2003/8/12/pubmed PY - 2004/5/13/medline PY - 2003/8/12/entrez SP - 169 EP - 74 JF - Archives of dermatological research JO - Arch Dermatol Res VL - 295 IS - 4 N2 - Prior studies in hairless mice have demonstrated that acute barrier disruption by acetone treatment increases the molecular weight (MW) cutoff of polyethylene glycol (PEG) penetration through the skin. The objective of the present study was to further investigate the dependence of permeability on MW with different forms of barrier disruption. A series of PEGs ranging in MW from near 300 to over 1000 Da were used to study the effects of tape stripping and sodium dodecyl sulfate (SDS) treatment on the MW permeability profiles of mouse skin in vitro. The 12-h percutaneous penetration of all the PEG 300, 600, and 1000 oligomers generally increased as a function of transepidermal water loss (TEWL) of the skin, either tape-stripped or SDS-treated. In addition, the total penetration of PEG oligomers across control skin, and skin tape-stripped and SDS-treated to different degrees of barrier disruption progressively decreased with increasing MW. There were no significant differences in the percutaneous penetration of the PEG oligomers between skin tape-stripped and SDS-treated to the same degree of barrier disruption. The penetration enhancement relative to control skin was more prominent with larger molecules. The MW cutoff for skin penetration increased with the degree of barrier disruption irrespective of the treatment applied, and was 986 Da (tape stripping) and 766 Da (SDS treatment) at TEWL levels in the range 10-20 g/m(2) per h in comparison with 414 Da for control skin. In accordance with previous findings in acetone-treated mouse skin, the results strongly suggest that, irrespective of the form of barrier disruption applied, not only higher amounts but also more varieties of chemicals (larger molecules) may penetrate skin with a compromised barrier than normal skin. SN - 0340-3696 UR - https://www.unboundmedicine.com/medline/citation/12910356/Tape_stripping_and_sodium_dodecyl_sulfate_treatment_increase_the_molecular_weight_cutoff_of_polyethylene_glycol_penetration_across_murine_skin_ DB - PRIME DP - Unbound Medicine ER -