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Th2- and to a lesser extent Th1-type cytokines upregulate the production of both CXC (IL-8 and gro-alpha) and CC (RANTES, eotaxin, eotaxin-2, MCP-3 and MCP-4) chemokines in human airway epithelial cells.
Int Arch Allergy Immunol. 2003 Aug; 131(4):264-71.IA

Abstract

BACKGROUND

Both CXC and CC chemokines play an important role in leukocyte recruitment. However, a systematic examination of their production by human airway epithelial cells (HAECs) has not been carried out. The objective of this study was to investigate whether Th1- and Th2-type cytokines regulate chemokine production in HAECs.

METHODS

HAECs were grown from both nasal and bronchial tissue and subsequently stimulated with either Th1- or Th2-type cytokines.

RESULTS

Constitutive mRNA expression for gro-alpha, IL-8 and RANTES was seen in both human nasal and human bronchial epithelial cells. IL-4 was the strongest stimulus for both gene expression and protein production of the chemokines RANTES, IL-8 and gro-alpha, while both IL-13 and IFN-gamma were weaker inducers of these chemokines, with the exception of gro-alpha (IL-13 was a strong stimulus for gro-alpha production). TNF-alpha synergized with IL-4, and to a lesser extent with IFN-gamma and IL-13, to release RANTES, IL-8 and gro-alpha. IL-4 and to a lesser extent IL-13 and IFN-gamma stimulated the production of MCP-3 and -4, eotaxin and eotaxin-2 immunoreactivities. However, no induction of the mRNAs encoding these chemokines was observed, suggesting that they may be released from a preformed pool within the HAECs.

CONCLUSION

These findings suggest that when released into the airways, Th2- and to a lesser extent Th1-type cytokines may stimulate recruitment of eosinophils and neutrophils through the release of CC (RANTES, MCP-3 and -4, eotaxin and eotaxin-2) and CXC chemokines (gro-alpha and IL-8).

Authors+Show Affiliations

Department of Dermatology, University of Kiel, Kiel, Germany. umeyerhoffert@dermatology.uni-kiel.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12915769

Citation

Meyer-Hoffert, Ulf, et al. "Th2- and to a Lesser Extent Th1-type Cytokines Upregulate the Production of Both CXC (IL-8 and Gro-alpha) and CC (RANTES, Eotaxin, Eotaxin-2, MCP-3 and MCP-4) Chemokines in Human Airway Epithelial Cells." International Archives of Allergy and Immunology, vol. 131, no. 4, 2003, pp. 264-71.
Meyer-Hoffert U, Lezcano-Meza D, Bartels J, et al. Th2- and to a lesser extent Th1-type cytokines upregulate the production of both CXC (IL-8 and gro-alpha) and CC (RANTES, eotaxin, eotaxin-2, MCP-3 and MCP-4) chemokines in human airway epithelial cells. Int Arch Allergy Immunol. 2003;131(4):264-71.
Meyer-Hoffert, U., Lezcano-Meza, D., Bartels, J., Montes-Vizuet, A. R., Schröder, J. M., & Teran, L. M. (2003). Th2- and to a lesser extent Th1-type cytokines upregulate the production of both CXC (IL-8 and gro-alpha) and CC (RANTES, eotaxin, eotaxin-2, MCP-3 and MCP-4) chemokines in human airway epithelial cells. International Archives of Allergy and Immunology, 131(4), 264-71.
Meyer-Hoffert U, et al. Th2- and to a Lesser Extent Th1-type Cytokines Upregulate the Production of Both CXC (IL-8 and Gro-alpha) and CC (RANTES, Eotaxin, Eotaxin-2, MCP-3 and MCP-4) Chemokines in Human Airway Epithelial Cells. Int Arch Allergy Immunol. 2003;131(4):264-71. PubMed PMID: 12915769.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Th2- and to a lesser extent Th1-type cytokines upregulate the production of both CXC (IL-8 and gro-alpha) and CC (RANTES, eotaxin, eotaxin-2, MCP-3 and MCP-4) chemokines in human airway epithelial cells. AU - Meyer-Hoffert,Ulf, AU - Lezcano-Meza,Diana, AU - Bartels,Joachim, AU - Montes-Vizuet,Aurea Rosalia, AU - Schröder,Jens-M, AU - Teran,Luis M, PY - 2002/10/14/received PY - 2003/05/20/accepted PY - 2003/8/14/pubmed PY - 2003/9/17/medline PY - 2003/8/14/entrez SP - 264 EP - 71 JF - International archives of allergy and immunology JO - Int. Arch. Allergy Immunol. VL - 131 IS - 4 N2 - BACKGROUND: Both CXC and CC chemokines play an important role in leukocyte recruitment. However, a systematic examination of their production by human airway epithelial cells (HAECs) has not been carried out. The objective of this study was to investigate whether Th1- and Th2-type cytokines regulate chemokine production in HAECs. METHODS: HAECs were grown from both nasal and bronchial tissue and subsequently stimulated with either Th1- or Th2-type cytokines. RESULTS: Constitutive mRNA expression for gro-alpha, IL-8 and RANTES was seen in both human nasal and human bronchial epithelial cells. IL-4 was the strongest stimulus for both gene expression and protein production of the chemokines RANTES, IL-8 and gro-alpha, while both IL-13 and IFN-gamma were weaker inducers of these chemokines, with the exception of gro-alpha (IL-13 was a strong stimulus for gro-alpha production). TNF-alpha synergized with IL-4, and to a lesser extent with IFN-gamma and IL-13, to release RANTES, IL-8 and gro-alpha. IL-4 and to a lesser extent IL-13 and IFN-gamma stimulated the production of MCP-3 and -4, eotaxin and eotaxin-2 immunoreactivities. However, no induction of the mRNAs encoding these chemokines was observed, suggesting that they may be released from a preformed pool within the HAECs. CONCLUSION: These findings suggest that when released into the airways, Th2- and to a lesser extent Th1-type cytokines may stimulate recruitment of eosinophils and neutrophils through the release of CC (RANTES, MCP-3 and -4, eotaxin and eotaxin-2) and CXC chemokines (gro-alpha and IL-8). SN - 1018-2438 UR - https://www.unboundmedicine.com/medline/citation/12915769/Th2__and_to_a_lesser_extent_Th1_type_cytokines_upregulate_the_production_of_both_CXC__IL_8_and_gro_alpha__and_CC__RANTES_eotaxin_eotaxin_2_MCP_3_and_MCP_4__chemokines_in_human_airway_epithelial_cells_ L2 - https://www.karger.com?DOI=10.1159/000072138 DB - PRIME DP - Unbound Medicine ER -