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Determination of proanthocyanidins in grape products by liquid chromatography/mass spectrometric detection under low collision energy.
Anal Chem. 2003 May 15; 75(10):2440-4.AC

Abstract

A method has been established for the identification of proanthocyanidins and quantification of individual monoproanthocyanidins using liquid chromatography/electrospray ionization-mass spectrometric detection (LC/ESI-MSD) for raw grape products. The separated monoproanthocyanidins and oligoproanthocyanidins were individually analyzed and identified by their molecular ion peaks using LC/MS. Using HPLC/ESI-MSD, the proanthocyanidin monomers, (+)-catechin (C), (-)-epicatechin (EC), (-)-catechin gallate (CG), and (-)-epicatechin gallate (ECG) in grape products were successfully quantified by LC/MS/MS detection of protonated molecular ions and characteristic fragment ions for each component under the optimized low collision energy level of 20%. For the investigated concentration ranges of C (21.88-11,200 ng/mL), EC (21.10-10,800 ng/mL), CG (36.72-18,800 ng/mL), and ECG (39.84-20,400 ng/mL), good linearities (r2 > 0.99) for standard curves were obtained. Validation of this method showed an accuracy that was well below 15% and precision (RSD) within 8% for the four compounds. The method proposed here is simple, sensitive, and allows a direct sample preparation procedure. This is the first method that enables the determination of individual monoproanthocyanidins in grape products without any solid-phase extraction.

Authors+Show Affiliations

New Use Agriculture and Natural Plant Products Program, Department of Plant Biology and Pathology, Cook College, Rutgers University, New Brunswick, New Jersey 08901-8520, USA. qlwu@aesop.rutgers.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12918988

Citation

Wu, Qingli, et al. "Determination of Proanthocyanidins in Grape Products By Liquid Chromatography/mass Spectrometric Detection Under Low Collision Energy." Analytical Chemistry, vol. 75, no. 10, 2003, pp. 2440-4.
Wu Q, Wang M, Simon JE. Determination of proanthocyanidins in grape products by liquid chromatography/mass spectrometric detection under low collision energy. Anal Chem. 2003;75(10):2440-4.
Wu, Q., Wang, M., & Simon, J. E. (2003). Determination of proanthocyanidins in grape products by liquid chromatography/mass spectrometric detection under low collision energy. Analytical Chemistry, 75(10), 2440-4.
Wu Q, Wang M, Simon JE. Determination of Proanthocyanidins in Grape Products By Liquid Chromatography/mass Spectrometric Detection Under Low Collision Energy. Anal Chem. 2003 May 15;75(10):2440-4. PubMed PMID: 12918988.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of proanthocyanidins in grape products by liquid chromatography/mass spectrometric detection under low collision energy. AU - Wu,Qingli, AU - Wang,Mingfu, AU - Simon,James E, PY - 2003/8/16/pubmed PY - 2003/10/22/medline PY - 2003/8/16/entrez SP - 2440 EP - 4 JF - Analytical chemistry JO - Anal Chem VL - 75 IS - 10 N2 - A method has been established for the identification of proanthocyanidins and quantification of individual monoproanthocyanidins using liquid chromatography/electrospray ionization-mass spectrometric detection (LC/ESI-MSD) for raw grape products. The separated monoproanthocyanidins and oligoproanthocyanidins were individually analyzed and identified by their molecular ion peaks using LC/MS. Using HPLC/ESI-MSD, the proanthocyanidin monomers, (+)-catechin (C), (-)-epicatechin (EC), (-)-catechin gallate (CG), and (-)-epicatechin gallate (ECG) in grape products were successfully quantified by LC/MS/MS detection of protonated molecular ions and characteristic fragment ions for each component under the optimized low collision energy level of 20%. For the investigated concentration ranges of C (21.88-11,200 ng/mL), EC (21.10-10,800 ng/mL), CG (36.72-18,800 ng/mL), and ECG (39.84-20,400 ng/mL), good linearities (r2 > 0.99) for standard curves were obtained. Validation of this method showed an accuracy that was well below 15% and precision (RSD) within 8% for the four compounds. The method proposed here is simple, sensitive, and allows a direct sample preparation procedure. This is the first method that enables the determination of individual monoproanthocyanidins in grape products without any solid-phase extraction. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/12918988/Determination_of_proanthocyanidins_in_grape_products_by_liquid_chromatography/mass_spectrometric_detection_under_low_collision_energy_ L2 - https://doi.org/10.1021/ac0262311 DB - PRIME DP - Unbound Medicine ER -
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