Tags

Type your tag names separated by a space and hit enter

Use of FTIR, FT-Raman and 13C-NMR spectroscopy for identification of some seaweed phycocolloids.
Biomol Eng. 2003 Jul; 20(4-6):223-8.BE

Abstract

Many seaweeds produce phycocolloids, stored in the cell wall. Members of the Rhodophyceae produce polysaccharides the main components of which are galactose (galactans)-agar and carrageenan. In addition, alginic acid is extracted from members of the Phaeophyceae. This is a binary polyuronide made up of mannuronic acid and guluronic acid. The wide uses of these phycocolloids are based on their gelling, viscosifying and emulsifying properties, which generate an increasing commercial and scientific interest. In this work, the FTIR and FT-RAMAN spectra of carrageenan and agar, obtained by alkaline extraction from different seaweeds (e.g. Mastocarpus stellatus, Chondrus crispus, Calliblepharis jubata, Chondracanthus acicularis, Chondracanthus teedei and Gracilaria gracilis), were recorded in order to identify the type of phycocolloid produced. The spectra of commercial carrageenan, alginic acid and agar samples (SIGMA and TAAB laboratories) were used as references. Special emphasis was given to the 500-1500 cm(-1) region, which presents several vibrational modes, sensitive to the type of polysaccharide and to the type of glycosidic linkage. The FT-Raman spectra present a higher resolution than FTIR spectra, this allowing the identification of a larger number of characteristic bands. In some cases, phycocolloids can be identified by FT-Raman spectroscopy alone.

Authors+Show Affiliations

Pereira L
Laboratório de M.E. e Ficologia, Departamento de Botânica, Universidade de Coimbra, 3000 Coimbra, Portugal. leonel@ci.uc.pt
Sousa A
No affiliation info available
Coelho H
No affiliation info available
Amado AM
No affiliation info available
Ribeiro-Claro PJ
No affiliation info available

MeSH

AgarAlginatesCarbon IsotopesCarrageenanColloidsMagnetic Resonance SpectroscopyPolysaccharidesReproductionRhodophytaSpecies SpecificitySpectrophotometry, InfraredSpectrum AnalysisSpectrum Analysis, Raman

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12919801

Citation

Pereira, Leonel, et al. "Use of FTIR, FT-Raman and 13C-NMR Spectroscopy for Identification of some Seaweed Phycocolloids." Biomolecular Engineering, vol. 20, no. 4-6, 2003, pp. 223-8.
Pereira L, Sousa A, Coelho H, et al. Use of FTIR, FT-Raman and 13C-NMR spectroscopy for identification of some seaweed phycocolloids. Biomol Eng. 2003;20(4-6):223-8.
Pereira, L., Sousa, A., Coelho, H., Amado, A. M., & Ribeiro-Claro, P. J. (2003). Use of FTIR, FT-Raman and 13C-NMR spectroscopy for identification of some seaweed phycocolloids. Biomolecular Engineering, 20(4-6), 223-8.
Pereira L, et al. Use of FTIR, FT-Raman and 13C-NMR Spectroscopy for Identification of some Seaweed Phycocolloids. Biomol Eng. 2003;20(4-6):223-8. PubMed PMID: 12919801.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of FTIR, FT-Raman and 13C-NMR spectroscopy for identification of some seaweed phycocolloids. AU - Pereira,Leonel, AU - Sousa,Ana, AU - Coelho,Helena, AU - Amado,Ana M, AU - Ribeiro-Claro,Paulo J A, PY - 2003/8/16/pubmed PY - 2004/5/28/medline PY - 2003/8/16/entrez SP - 223 EP - 8 JF - Biomolecular engineering JO - Biomol Eng VL - 20 IS - 4-6 N2 - Many seaweeds produce phycocolloids, stored in the cell wall. Members of the Rhodophyceae produce polysaccharides the main components of which are galactose (galactans)-agar and carrageenan. In addition, alginic acid is extracted from members of the Phaeophyceae. This is a binary polyuronide made up of mannuronic acid and guluronic acid. The wide uses of these phycocolloids are based on their gelling, viscosifying and emulsifying properties, which generate an increasing commercial and scientific interest. In this work, the FTIR and FT-RAMAN spectra of carrageenan and agar, obtained by alkaline extraction from different seaweeds (e.g. Mastocarpus stellatus, Chondrus crispus, Calliblepharis jubata, Chondracanthus acicularis, Chondracanthus teedei and Gracilaria gracilis), were recorded in order to identify the type of phycocolloid produced. The spectra of commercial carrageenan, alginic acid and agar samples (SIGMA and TAAB laboratories) were used as references. Special emphasis was given to the 500-1500 cm(-1) region, which presents several vibrational modes, sensitive to the type of polysaccharide and to the type of glycosidic linkage. The FT-Raman spectra present a higher resolution than FTIR spectra, this allowing the identification of a larger number of characteristic bands. In some cases, phycocolloids can be identified by FT-Raman spectroscopy alone. SN - 1389-0344 UR - https://www.unboundmedicine.com/medline/citation/12919801/Use_of_FTIR_FT_Raman_and_13C_NMR_spectroscopy_for_identification_of_some_seaweed_phycocolloids_ DB - PRIME DP - Unbound Medicine ER -