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[1st environmental isolation of Cryptococcus neoformans var. gattii in Argentina].
Rev Argent Microbiol. 2003 Apr-Jun; 35(2):110-2.RA

Abstract

Two strains of C. neoformans var. gattii serotype B were isolated from a park in Buenos Aires city, Argentina. Samples were collected in spring, rubbing with swabs on the inner hollow of Eucalyptus spp. and other trees, which were not identified at the collection moment. Samples were swabbed directly onto cafeic acid with antibiotic agar, then incubated at 28 degrees C and observed daily for a week. Isolates were recovered from cafeic acid medium showing characteristic colonies of the species that allowed differentiation from other microorganisms of the sample. Identification was performed using carbohydrate fermentation, assimilation of nitrogen and carbon sources, and urease and phenoloxidase detection. Strain variety was determined with canavanine-glicine-bromotimol blue agar medium (CGB). Genotypic characterization using AP-PCR with (GACA)4 aleatory primer showed that both band profiles could be differentiated by only one band. These two strains were isolated from tree species different from Eucalyptus spp. These isolates from natural sources demonstrate that C. neoformans var. gattii efosts in Buenos Aires city environment and that it is not necessarily associated with Eucalyptus spp. The presence of C. neoformansvar. gattii in the environment, detected for the first time in Argentina, may explain its sporadic association with human infections in the local population.

Authors+Show Affiliations

Davel G
Departamento Micología INEI ANLIS Dr. Carlos G. Malbrán, Av. Vélez Sarsfield 563, 1281 Buenos Aires, Argentina. gdavel@anlis.gov.ar
Abrantes R
No affiliation info available
Brudny M
No affiliation info available
Córdoba S
No affiliation info available
Rodero L
No affiliation info available
Canteros CE
No affiliation info available
Perrotta D
No affiliation info available

MeSH

ArgentinaCryptococcosisCryptococcus neoformansDisease ReservoirsEnvironmental MicrobiologyHumansMycologyRecreationTrees

Pub Type(s)

Journal Article
Review

Language

spa

PubMed ID

12920995

Citation

Davel, G, et al. "[1st Environmental Isolation of Cryptococcus Neoformans Var. Gattii in Argentina]." Revista Argentina De Microbiologia, vol. 35, no. 2, 2003, pp. 110-2.
Davel G, Abrantes R, Brudny M, et al. [1st environmental isolation of Cryptococcus neoformans var. gattii in Argentina]. Rev Argent Microbiol. 2003;35(2):110-2.
Davel, G., Abrantes, R., Brudny, M., Córdoba, S., Rodero, L., Canteros, C. E., & Perrotta, D. (2003). [1st environmental isolation of Cryptococcus neoformans var. gattii in Argentina]. Revista Argentina De Microbiologia, 35(2), 110-2.
Davel G, et al. [1st Environmental Isolation of Cryptococcus Neoformans Var. Gattii in Argentina]. Rev Argent Microbiol. 2003 Apr-Jun;35(2):110-2. PubMed PMID: 12920995.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [1st environmental isolation of Cryptococcus neoformans var. gattii in Argentina]. AU - Davel,G, AU - Abrantes,R, AU - Brudny,M, AU - Córdoba,S, AU - Rodero,L, AU - Canteros,C E, AU - Perrotta,D, PY - 2003/8/19/pubmed PY - 2003/10/28/medline PY - 2003/8/19/entrez SP - 110 EP - 2 JF - Revista Argentina de microbiologia JO - Rev Argent Microbiol VL - 35 IS - 2 N2 - Two strains of C. neoformans var. gattii serotype B were isolated from a park in Buenos Aires city, Argentina. Samples were collected in spring, rubbing with swabs on the inner hollow of Eucalyptus spp. and other trees, which were not identified at the collection moment. Samples were swabbed directly onto cafeic acid with antibiotic agar, then incubated at 28 degrees C and observed daily for a week. Isolates were recovered from cafeic acid medium showing characteristic colonies of the species that allowed differentiation from other microorganisms of the sample. Identification was performed using carbohydrate fermentation, assimilation of nitrogen and carbon sources, and urease and phenoloxidase detection. Strain variety was determined with canavanine-glicine-bromotimol blue agar medium (CGB). Genotypic characterization using AP-PCR with (GACA)4 aleatory primer showed that both band profiles could be differentiated by only one band. These two strains were isolated from tree species different from Eucalyptus spp. These isolates from natural sources demonstrate that C. neoformans var. gattii efosts in Buenos Aires city environment and that it is not necessarily associated with Eucalyptus spp. The presence of C. neoformansvar. gattii in the environment, detected for the first time in Argentina, may explain its sporadic association with human infections in the local population. SN - 0325-7541 UR - https://www.unboundmedicine.com/medline/citation/12920995/[1st_environmental_isolation_of_Cryptococcus_neoformans_var__gattii_in_Argentina]_ DB - PRIME DP - Unbound Medicine ER -