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Regional gastrointestinal permeability of rifampicin and isoniazid (alone and their combination) in the rat.
Int J Tuberc Lung Dis. 2003 Aug; 7(8):797-803.IJ

Abstract

OBJECTIVE

To determine if rifampicin (RMP) and isoniazid (INH) are absorbed from different gastrointestinal tract (GIT) sites, and to ascertain the feasibility of producing new fixed-dose combination (FDC) products containing RMP and INH by segregating drug delivery at different sites along the GIT.

DESIGN

Permeability of RMP and INH (alone and in combination) was determined in various segments of rat GIT (stomach, duodenum, jejunum and ileum) at concentrations of respectively 2.4 and 1.2 mg/ml, using a ligated loop technique. Drug analysis was performed by HPLC. Extent of absorption was considered as the total drug disappearing from the loop. Permeability was correlated with solubility and decomposition data at pH corresponding to different GIT sites.

RESULTS

RMP was well absorbed from the stomach due to its solubility, which was maximum between pH 1-2. INH was poorly absorbed from the stomach, but was well absorbed from all three segments of the intestine. In combination, RMP disappearance was enhanced in the presence of INH in the stomach and jejunum, but INH disappearance was not influenced by RMP.

CONCLUSION

The study shows higher in situ RMP disappearance in the presence of INH, attributable to drug degradation due to catalysis by INH. As the two drugs show regional specific permeability, FDCs without reduced RMP bioavailability resulting from its decomposition in the presence of INH can be designed by segregating delivery of the two drugs by around 3-4 h. RMP should be released in the stomach and INH in the intestine.

Authors+Show Affiliations

Department of Pharmaceutical Analysis, NIPER, SAS Nagar, India.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12921157

Citation

Mariappan, T T., and S Singh. "Regional Gastrointestinal Permeability of Rifampicin and Isoniazid (alone and Their Combination) in the Rat." The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease, vol. 7, no. 8, 2003, pp. 797-803.
Mariappan TT, Singh S. Regional gastrointestinal permeability of rifampicin and isoniazid (alone and their combination) in the rat. Int J Tuberc Lung Dis. 2003;7(8):797-803.
Mariappan, T. T., & Singh, S. (2003). Regional gastrointestinal permeability of rifampicin and isoniazid (alone and their combination) in the rat. The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease, 7(8), 797-803.
Mariappan TT, Singh S. Regional Gastrointestinal Permeability of Rifampicin and Isoniazid (alone and Their Combination) in the Rat. Int J Tuberc Lung Dis. 2003;7(8):797-803. PubMed PMID: 12921157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regional gastrointestinal permeability of rifampicin and isoniazid (alone and their combination) in the rat. AU - Mariappan,T T, AU - Singh,S, PY - 2003/8/19/pubmed PY - 2003/9/25/medline PY - 2003/8/19/entrez SP - 797 EP - 803 JF - The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease JO - Int. J. Tuberc. Lung Dis. VL - 7 IS - 8 N2 - OBJECTIVE: To determine if rifampicin (RMP) and isoniazid (INH) are absorbed from different gastrointestinal tract (GIT) sites, and to ascertain the feasibility of producing new fixed-dose combination (FDC) products containing RMP and INH by segregating drug delivery at different sites along the GIT. DESIGN: Permeability of RMP and INH (alone and in combination) was determined in various segments of rat GIT (stomach, duodenum, jejunum and ileum) at concentrations of respectively 2.4 and 1.2 mg/ml, using a ligated loop technique. Drug analysis was performed by HPLC. Extent of absorption was considered as the total drug disappearing from the loop. Permeability was correlated with solubility and decomposition data at pH corresponding to different GIT sites. RESULTS: RMP was well absorbed from the stomach due to its solubility, which was maximum between pH 1-2. INH was poorly absorbed from the stomach, but was well absorbed from all three segments of the intestine. In combination, RMP disappearance was enhanced in the presence of INH in the stomach and jejunum, but INH disappearance was not influenced by RMP. CONCLUSION: The study shows higher in situ RMP disappearance in the presence of INH, attributable to drug degradation due to catalysis by INH. As the two drugs show regional specific permeability, FDCs without reduced RMP bioavailability resulting from its decomposition in the presence of INH can be designed by segregating delivery of the two drugs by around 3-4 h. RMP should be released in the stomach and INH in the intestine. SN - 1027-3719 UR - https://www.unboundmedicine.com/medline/citation/12921157/Regional_gastrointestinal_permeability_of_rifampicin_and_isoniazid__alone_and_their_combination__in_the_rat_ L2 - https://www.ingentaconnect.com/openurl?genre=article&issn=1027-3719&volume=7&issue=8&spage=797&aulast=Mariappan DB - PRIME DP - Unbound Medicine ER -