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[The role of matrix metalloproteinases-9 and tissue inhibitor of metalloproteinaes-1 in chronic obstructive pulmonary disease].
Zhonghua Yi Xue Za Zhi. 2003 Jul 10; 83(13):1138-41.ZY

Abstract

OBJECTIVE

To evaluate the role of matrix metalloproteinases-9 (MMP-9) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in the pathogenesis of chronic obstructive pulmonary disease (COPD).

METHODS

Five milliliters of peripheral venous blood were extracted form 36 patients with COPD, subdivided into stable group (CS group, n = 12), exacerbation group (CE group, n = 12), and exacerbation group with glucocorticosteroids treatment (CE + G group, n = 12), and 12 normal controls. The peripheral blood mononuclear cells (PBMCs) were isolated and the mRNA expressions of MMP-9 and TIMP-1 therein in each group were measured by RT-PCR quantitative analysis. The MMP-2 and MMP-9 levels in the plasma were studied by gelatin zymography. The relationship between MMP and pulmonary function was evaluated.

RESULTS

(1) The MMP-9 mRNA expression and MMP-9: TIMP-1 ratios in the samples obtained from patients with COPD were higher in comparison with those from the control group (all P < 0.05). (2) The MMP-9 levels in plasma were higher in the COPD groups than in the control group (P < 0.05). (3) In the COPD groups, the MMP-9 mRNA expression, MMP-9: TIMP-1 ratio, and MMP-9 level in plasma were negatively correlated with FEV(1.0)% of prediction (r = -0.789, P < 0.05; r = -0.53, P < 0.05; and r = -0.652, P < 0.05), and positively correlated with RV/TLC% (r = 0.375, P < 0.05; r = 0.44, P < 0.05; and r = 0.529, P < 0.05).

CONCLUSION

MMP-9 and the imbalanced ratio of MMP-9: TIMP-1 may not only play a role in the pathogenesis of COPD, but also relate to FEV(1.0)% of prediction and RV/TLC%.

Authors+Show Affiliations

Beijing Hospital, Beijing 100730, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12921631

Citation

Xu, Xiao-mao, et al. "[The Role of Matrix Metalloproteinases-9 and Tissue Inhibitor of Metalloproteinaes-1 in Chronic Obstructive Pulmonary Disease]." Zhonghua Yi Xue Za Zhi, vol. 83, no. 13, 2003, pp. 1138-41.
Xu XM, Sun TY, Lin JB, et al. [The role of matrix metalloproteinases-9 and tissue inhibitor of metalloproteinaes-1 in chronic obstructive pulmonary disease]. Zhonghua Yi Xue Za Zhi. 2003;83(13):1138-41.
Xu, X. M., Sun, T. Y., Lin, J. B., & Zhang, H. S. (2003). [The role of matrix metalloproteinases-9 and tissue inhibitor of metalloproteinaes-1 in chronic obstructive pulmonary disease]. Zhonghua Yi Xue Za Zhi, 83(13), 1138-41.
Xu XM, et al. [The Role of Matrix Metalloproteinases-9 and Tissue Inhibitor of Metalloproteinaes-1 in Chronic Obstructive Pulmonary Disease]. Zhonghua Yi Xue Za Zhi. 2003 Jul 10;83(13):1138-41. PubMed PMID: 12921631.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The role of matrix metalloproteinases-9 and tissue inhibitor of metalloproteinaes-1 in chronic obstructive pulmonary disease]. AU - Xu,Xiao-mao, AU - Sun,Tie-ying, AU - Lin,Jia-bin, AU - Zhang,Hong-sheng, PY - 2003/8/19/pubmed PY - 2004/2/3/medline PY - 2003/8/19/entrez SP - 1138 EP - 41 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 83 IS - 13 N2 - OBJECTIVE: To evaluate the role of matrix metalloproteinases-9 (MMP-9) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in the pathogenesis of chronic obstructive pulmonary disease (COPD). METHODS: Five milliliters of peripheral venous blood were extracted form 36 patients with COPD, subdivided into stable group (CS group, n = 12), exacerbation group (CE group, n = 12), and exacerbation group with glucocorticosteroids treatment (CE + G group, n = 12), and 12 normal controls. The peripheral blood mononuclear cells (PBMCs) were isolated and the mRNA expressions of MMP-9 and TIMP-1 therein in each group were measured by RT-PCR quantitative analysis. The MMP-2 and MMP-9 levels in the plasma were studied by gelatin zymography. The relationship between MMP and pulmonary function was evaluated. RESULTS: (1) The MMP-9 mRNA expression and MMP-9: TIMP-1 ratios in the samples obtained from patients with COPD were higher in comparison with those from the control group (all P < 0.05). (2) The MMP-9 levels in plasma were higher in the COPD groups than in the control group (P < 0.05). (3) In the COPD groups, the MMP-9 mRNA expression, MMP-9: TIMP-1 ratio, and MMP-9 level in plasma were negatively correlated with FEV(1.0)% of prediction (r = -0.789, P < 0.05; r = -0.53, P < 0.05; and r = -0.652, P < 0.05), and positively correlated with RV/TLC% (r = 0.375, P < 0.05; r = 0.44, P < 0.05; and r = 0.529, P < 0.05). CONCLUSION: MMP-9 and the imbalanced ratio of MMP-9: TIMP-1 may not only play a role in the pathogenesis of COPD, but also relate to FEV(1.0)% of prediction and RV/TLC%. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/12921631/[The_role_of_matrix_metalloproteinases_9_and_tissue_inhibitor_of_metalloproteinaes_1_in_chronic_obstructive_pulmonary_disease]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0376-2491&amp;year=2003&amp;vol=83&amp;issue=13&amp;fpage=1138 DB - PRIME DP - Unbound Medicine ER -