Tags

Type your tag names separated by a space and hit enter

Cannabinoid CB1 receptor inhibition of mechanically evoked responses of spinal neurones in control rats, but not in rats with hindpaw inflammation.
Eur J Pharmacol. 2003 Aug 08; 474(2-3):209-16.EJ

Abstract

Spinally administered cannabinoid receptor agonists are anti-nociceptive in a variety of models of acute and persistent pain. The present study investigated the effects of activation of spinal cannabinoid CB(1) receptors on mechanically evoked responses of spinal neurones in acute and inflammatory pain states. In vivo electrophysiology studies were carried out in anaesthetised rats. Effects of spinal administration of a selective cannabinoid CB(1) receptor agonist, arachidonyl-2-chloroethylamide (ACEA), on mechanically evoked responses of dorsal horn neurones in control rats and rats with peripheral hindpaw carrageenan-induced inflammation were compared. ACEA (0.27 nM-27 microM) significantly inhibited innocuous and noxious mechanically evoked responses of dorsal horn neurones in control rats. Pre-administration of the CB(1) receptor antagonist N-(piperidin-1-yl)-5-(4-chlorophenyl)-1(2,4-dichlorophenyl)-4-methyl-1-H-pyrazole-3-carboxyamide, SR141716A, (0.43 microM) attenuated the inhibitory effects of ACEA (27 microM). ACEA did not alter mechanically evoked responses of dorsal horn neurones in rats with hindpaw carrageenan-induced inflammation. Following peripheral inflammation, there is a loss of spinal CB(1) receptor-mediated inhibition of mechanically evoked responses, which is suggestive of a functional down-regulation of CB(1) receptors under these conditions.

Authors+Show Affiliations

University of Nottingham Medical School, Queen's Medical Centre, NG7 2UH Nottingham, UK.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12921864

Citation

Kelly, Sara, and Victoria Chapman. "Cannabinoid CB1 Receptor Inhibition of Mechanically Evoked Responses of Spinal Neurones in Control Rats, but Not in Rats With Hindpaw Inflammation." European Journal of Pharmacology, vol. 474, no. 2-3, 2003, pp. 209-16.
Kelly S, Chapman V. Cannabinoid CB1 receptor inhibition of mechanically evoked responses of spinal neurones in control rats, but not in rats with hindpaw inflammation. Eur J Pharmacol. 2003;474(2-3):209-16.
Kelly, S., & Chapman, V. (2003). Cannabinoid CB1 receptor inhibition of mechanically evoked responses of spinal neurones in control rats, but not in rats with hindpaw inflammation. European Journal of Pharmacology, 474(2-3), 209-16.
Kelly S, Chapman V. Cannabinoid CB1 Receptor Inhibition of Mechanically Evoked Responses of Spinal Neurones in Control Rats, but Not in Rats With Hindpaw Inflammation. Eur J Pharmacol. 2003 Aug 8;474(2-3):209-16. PubMed PMID: 12921864.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cannabinoid CB1 receptor inhibition of mechanically evoked responses of spinal neurones in control rats, but not in rats with hindpaw inflammation. AU - Kelly,Sara, AU - Chapman,Victoria, PY - 2003/8/19/pubmed PY - 2004/4/24/medline PY - 2003/8/19/entrez SP - 209 EP - 16 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 474 IS - 2-3 N2 - Spinally administered cannabinoid receptor agonists are anti-nociceptive in a variety of models of acute and persistent pain. The present study investigated the effects of activation of spinal cannabinoid CB(1) receptors on mechanically evoked responses of spinal neurones in acute and inflammatory pain states. In vivo electrophysiology studies were carried out in anaesthetised rats. Effects of spinal administration of a selective cannabinoid CB(1) receptor agonist, arachidonyl-2-chloroethylamide (ACEA), on mechanically evoked responses of dorsal horn neurones in control rats and rats with peripheral hindpaw carrageenan-induced inflammation were compared. ACEA (0.27 nM-27 microM) significantly inhibited innocuous and noxious mechanically evoked responses of dorsal horn neurones in control rats. Pre-administration of the CB(1) receptor antagonist N-(piperidin-1-yl)-5-(4-chlorophenyl)-1(2,4-dichlorophenyl)-4-methyl-1-H-pyrazole-3-carboxyamide, SR141716A, (0.43 microM) attenuated the inhibitory effects of ACEA (27 microM). ACEA did not alter mechanically evoked responses of dorsal horn neurones in rats with hindpaw carrageenan-induced inflammation. Following peripheral inflammation, there is a loss of spinal CB(1) receptor-mediated inhibition of mechanically evoked responses, which is suggestive of a functional down-regulation of CB(1) receptors under these conditions. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/12921864/Cannabinoid_CB1_receptor_inhibition_of_mechanically_evoked_responses_of_spinal_neurones_in_control_rats_but_not_in_rats_with_hindpaw_inflammation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014299903020855 DB - PRIME DP - Unbound Medicine ER -