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RtsA and RtsB coordinately regulate expression of the invasion and flagellar genes in Salmonella enterica serovar Typhimurium.
J Bacteriol. 2003 Sep; 185(17):5096-108.JB

Abstract

Salmonella enterica serovar Typhimurium encounters numerous host environments and defense mechanisms during the infection process. The bacterium responds by tightly regulating the expression of virulence genes. We identified two regulatory proteins, termed RtsA and RtsB, which are encoded in an operon located on an island integrated at tRNA(PheU) in S. enterica serovar Typhimurium. RtsA belongs to the AraC/XylS family of regulators, and RtsB is a helix-turn-helix DNA binding protein. In a random screen, we identified five RtsA-regulated fusions, all belonging to the Salmonella pathogenicity island 1 (SPI1) regulon, which encodes a type III secretion system (TTSS) required for invasion of epithelial cells. We show that RtsA increases expression of the invasion genes by inducing hilA expression. RtsA also induces expression of hilD, hilC, and the invF operon. However, induction of hilA is independent of HilC and HilD and is mediated by direct binding of RtsA to the hilA promoter. The phenotype of an rtsA null mutation is similar to the phenotype of a hilC mutation, both of which decrease expression of SPI1 genes approximately twofold. We also show that RtsA can induce expression of a SPI1 TTSS effector, slrP, independent of any SPI1 regulatory protein. RtsB represses expression of the flagellar genes by binding to the flhDC promoter region. Repression of the positive activators flhDC decreases expression of the entire flagellar regulon. We propose that RtsA and RtsB coordinate induction of invasion and repression of motility in the small intestine.

Authors+Show Affiliations

Department of Microbiology, College of Medicine, Chemical and Life Sciences Laboratory, 601 S. Goodwin Avenue, University of Illinois, Urbana, IL 61801, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12923082

Citation

Ellermeier, Craig D., and James M. Slauch. "RtsA and RtsB Coordinately Regulate Expression of the Invasion and Flagellar Genes in Salmonella Enterica Serovar Typhimurium." Journal of Bacteriology, vol. 185, no. 17, 2003, pp. 5096-108.
Ellermeier CD, Slauch JM. RtsA and RtsB coordinately regulate expression of the invasion and flagellar genes in Salmonella enterica serovar Typhimurium. J Bacteriol. 2003;185(17):5096-108.
Ellermeier, C. D., & Slauch, J. M. (2003). RtsA and RtsB coordinately regulate expression of the invasion and flagellar genes in Salmonella enterica serovar Typhimurium. Journal of Bacteriology, 185(17), 5096-108.
Ellermeier CD, Slauch JM. RtsA and RtsB Coordinately Regulate Expression of the Invasion and Flagellar Genes in Salmonella Enterica Serovar Typhimurium. J Bacteriol. 2003;185(17):5096-108. PubMed PMID: 12923082.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RtsA and RtsB coordinately regulate expression of the invasion and flagellar genes in Salmonella enterica serovar Typhimurium. AU - Ellermeier,Craig D, AU - Slauch,James M, PY - 2003/8/19/pubmed PY - 2003/9/23/medline PY - 2003/8/19/entrez SP - 5096 EP - 108 JF - Journal of bacteriology JO - J Bacteriol VL - 185 IS - 17 N2 - Salmonella enterica serovar Typhimurium encounters numerous host environments and defense mechanisms during the infection process. The bacterium responds by tightly regulating the expression of virulence genes. We identified two regulatory proteins, termed RtsA and RtsB, which are encoded in an operon located on an island integrated at tRNA(PheU) in S. enterica serovar Typhimurium. RtsA belongs to the AraC/XylS family of regulators, and RtsB is a helix-turn-helix DNA binding protein. In a random screen, we identified five RtsA-regulated fusions, all belonging to the Salmonella pathogenicity island 1 (SPI1) regulon, which encodes a type III secretion system (TTSS) required for invasion of epithelial cells. We show that RtsA increases expression of the invasion genes by inducing hilA expression. RtsA also induces expression of hilD, hilC, and the invF operon. However, induction of hilA is independent of HilC and HilD and is mediated by direct binding of RtsA to the hilA promoter. The phenotype of an rtsA null mutation is similar to the phenotype of a hilC mutation, both of which decrease expression of SPI1 genes approximately twofold. We also show that RtsA can induce expression of a SPI1 TTSS effector, slrP, independent of any SPI1 regulatory protein. RtsB represses expression of the flagellar genes by binding to the flhDC promoter region. Repression of the positive activators flhDC decreases expression of the entire flagellar regulon. We propose that RtsA and RtsB coordinate induction of invasion and repression of motility in the small intestine. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/12923082/RtsA_and_RtsB_coordinately_regulate_expression_of_the_invasion_and_flagellar_genes_in_Salmonella_enterica_serovar_Typhimurium_ DB - PRIME DP - Unbound Medicine ER -