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Effect of GnRH analogues on apoptosis and release of interleukin-1beta and vascular endothelial growth factor in endometrial cell cultures from patients with endometriosis.
Hum Reprod. 2003 Sep; 18(9):1767-71.HR

Abstract

BACKGROUND

The aim of the present study was to evaluate the effect of GnRH analogues on the in-vitro eutopic endometrial cell apoptosis and release of interleukin-1beta (IL-1beta) and vascular endothelial growth factor (VEGF).

METHODS

Biopsy specimens of eutopic endometrium obtained from 16 women with untreated endometriosis and 14 controls were studied. Apoptosis, IL-1beta and VEGF release were evaluated in epithelial endometrial cell cultures after incubation with leuprolide acetate (LA) as GnRH agonist, antide as GnRH antagonist, and a combination of both. The percentage of apoptotic cells was evaluated by the acridine orange-ethidium bromide technique, and IL-1beta and VEGF concentrations were assessed by using commercial enzyme-linked immunosorbent assay (ELISA) kits.

RESULTS

We found that LA (100 ng/ml) enhanced apoptosis in endometrial cell cultures from endometriosis patients and controls and this effect was reversed by antide at 10(-7) mol/l. IL-1beta and VEGF release was downregulated by LA in cultures from controls and endometriosis patients. The addition of antide 10(-7) mol/l reversed this inhibition. Endometrial cultures treated with antide at 10(-7) mol/l did not show any significant effects compared with basal conditions.

CONCLUSIONS

GnRH agonists appear to have a direct effect in endometrial cells cultures, by enhancing the percentage of apoptotic cells and decreasing the release of pro-mitogenic cytokines such as IL-1beta and VEGF.

Authors+Show Affiliations

Instituto de Biología y Medicina Experimental (IBYME-CONICET), Vuelta de Obligado 2490, (C1428DNA) Buenos Aires, Argentina. meresman@dna.uba.arNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12923126

Citation

Meresman, Gabriela F., et al. "Effect of GnRH Analogues On Apoptosis and Release of Interleukin-1beta and Vascular Endothelial Growth Factor in Endometrial Cell Cultures From Patients With Endometriosis." Human Reproduction (Oxford, England), vol. 18, no. 9, 2003, pp. 1767-71.
Meresman GF, Bilotas MA, Lombardi E, et al. Effect of GnRH analogues on apoptosis and release of interleukin-1beta and vascular endothelial growth factor in endometrial cell cultures from patients with endometriosis. Hum Reprod. 2003;18(9):1767-71.
Meresman, G. F., Bilotas, M. A., Lombardi, E., Tesone, M., Sueldo, C., & Barañao, R. I. (2003). Effect of GnRH analogues on apoptosis and release of interleukin-1beta and vascular endothelial growth factor in endometrial cell cultures from patients with endometriosis. Human Reproduction (Oxford, England), 18(9), 1767-71.
Meresman GF, et al. Effect of GnRH Analogues On Apoptosis and Release of Interleukin-1beta and Vascular Endothelial Growth Factor in Endometrial Cell Cultures From Patients With Endometriosis. Hum Reprod. 2003;18(9):1767-71. PubMed PMID: 12923126.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of GnRH analogues on apoptosis and release of interleukin-1beta and vascular endothelial growth factor in endometrial cell cultures from patients with endometriosis. AU - Meresman,Gabriela F, AU - Bilotas,Mariela A, AU - Lombardi,Eduardo, AU - Tesone,Marta, AU - Sueldo,Carlos, AU - Barañao,Rosa I, PY - 2003/8/19/pubmed PY - 2004/4/22/medline PY - 2003/8/19/entrez SP - 1767 EP - 71 JF - Human reproduction (Oxford, England) JO - Hum Reprod VL - 18 IS - 9 N2 - BACKGROUND: The aim of the present study was to evaluate the effect of GnRH analogues on the in-vitro eutopic endometrial cell apoptosis and release of interleukin-1beta (IL-1beta) and vascular endothelial growth factor (VEGF). METHODS: Biopsy specimens of eutopic endometrium obtained from 16 women with untreated endometriosis and 14 controls were studied. Apoptosis, IL-1beta and VEGF release were evaluated in epithelial endometrial cell cultures after incubation with leuprolide acetate (LA) as GnRH agonist, antide as GnRH antagonist, and a combination of both. The percentage of apoptotic cells was evaluated by the acridine orange-ethidium bromide technique, and IL-1beta and VEGF concentrations were assessed by using commercial enzyme-linked immunosorbent assay (ELISA) kits. RESULTS: We found that LA (100 ng/ml) enhanced apoptosis in endometrial cell cultures from endometriosis patients and controls and this effect was reversed by antide at 10(-7) mol/l. IL-1beta and VEGF release was downregulated by LA in cultures from controls and endometriosis patients. The addition of antide 10(-7) mol/l reversed this inhibition. Endometrial cultures treated with antide at 10(-7) mol/l did not show any significant effects compared with basal conditions. CONCLUSIONS: GnRH agonists appear to have a direct effect in endometrial cells cultures, by enhancing the percentage of apoptotic cells and decreasing the release of pro-mitogenic cytokines such as IL-1beta and VEGF. SN - 0268-1161 UR - https://www.unboundmedicine.com/medline/citation/12923126/Effect_of_GnRH_analogues_on_apoptosis_and_release_of_interleukin_1beta_and_vascular_endothelial_growth_factor_in_endometrial_cell_cultures_from_patients_with_endometriosis_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/deg356 DB - PRIME DP - Unbound Medicine ER -