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[Establishment of keratinocyte-specific Cre recombinase transgenic mice].
Yi Chuan Xue Bao. 2003 May; 30(5):407-13.YC

Abstract

A keratinocyte-specific Cre transgenic construct (pK5-Cre) containing the keratin 5 promoter, Cre recombinase gene and polyA of human growth hormone gene was generated. The 4.2 kb DNA fragment of K5-Cre-hGH was introduced into 720 fertilized zygotes by microinjection. 695 injected eggs were implanted into the oviduct of 29 female mice respectively, from which 48 off-spring were obtained. Twelve mice carrying the transgene were identified by genotyping, and the integration efficiency is 25%. The K5-Cre transgenic mice were crossed with Smad4 conditional gene targeting mice to check the tissue-specific expression of the Cre recombinase and the Cre mediated recombination in multiple tissues. The results showed that the Cre recombinase was expressed in skin tissue only and successfully mediated the recombination between the loxP sites in vivo.

Authors+Show Affiliations

Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12924153

Citation

Mao, Chun-Ming, et al. "[Establishment of Keratinocyte-specific Cre Recombinase Transgenic Mice]." Yi Chuan Xue Bao = Acta Genetica Sinica, vol. 30, no. 5, 2003, pp. 407-13.
Mao CM, Yang X, Cheng X, et al. [Establishment of keratinocyte-specific Cre recombinase transgenic mice]. Yi Chuan Xue Bao. 2003;30(5):407-13.
Mao, C. M., Yang, X., Cheng, X., Lü, Y. X., Zhou, J., & Huang, C. F. (2003). [Establishment of keratinocyte-specific Cre recombinase transgenic mice]. Yi Chuan Xue Bao = Acta Genetica Sinica, 30(5), 407-13.
Mao CM, et al. [Establishment of Keratinocyte-specific Cre Recombinase Transgenic Mice]. Yi Chuan Xue Bao. 2003;30(5):407-13. PubMed PMID: 12924153.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Establishment of keratinocyte-specific Cre recombinase transgenic mice]. AU - Mao,Chun-Ming, AU - Yang,Xiao, AU - Cheng,Xuan, AU - Lü,Ya-Xin, AU - Zhou,Jiang, AU - Huang,Cui-Fen, PY - 2003/8/20/pubmed PY - 2003/12/3/medline PY - 2003/8/20/entrez SP - 407 EP - 13 JF - Yi chuan xue bao = Acta genetica Sinica JO - Yi Chuan Xue Bao VL - 30 IS - 5 N2 - A keratinocyte-specific Cre transgenic construct (pK5-Cre) containing the keratin 5 promoter, Cre recombinase gene and polyA of human growth hormone gene was generated. The 4.2 kb DNA fragment of K5-Cre-hGH was introduced into 720 fertilized zygotes by microinjection. 695 injected eggs were implanted into the oviduct of 29 female mice respectively, from which 48 off-spring were obtained. Twelve mice carrying the transgene were identified by genotyping, and the integration efficiency is 25%. The K5-Cre transgenic mice were crossed with Smad4 conditional gene targeting mice to check the tissue-specific expression of the Cre recombinase and the Cre mediated recombination in multiple tissues. The results showed that the Cre recombinase was expressed in skin tissue only and successfully mediated the recombination between the loxP sites in vivo. SN - 0379-4172 UR - https://www.unboundmedicine.com/medline/citation/12924153/[Establishment_of_keratinocyte_specific_Cre_recombinase_transgenic_mice]_ L2 - http://www.komp.org/ncbi.php?PMID=12924153 DB - PRIME DP - Unbound Medicine ER -