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Nuclear thioredoxin peroxidase Dot5 in Saccharomyces cerevisiae: roles in oxidative stress response and disruption of telomeric silencing.
Appl Microbiol Biotechnol. 2004 Mar; 64(1):120-4.AM

Abstract

The DOT5 gene was originally cloned as one of the DOT (disrupter of telomeric silencing) genes; and later it was re-discovered as a nuclear thioredoxin peroxidase in Saccharomyces cerevisiae. Here, we demonstrate that the telomeric-silencing disruption activity of Dot5 is independent of thioredoxin peroxidase activity. In addition, Dot5 cannot suppress the increased susceptibility to peroxides of mutants defected in cytosolic thioredoxin peroxidase, even when Dot5 is expressed in the cytoplasm. Furthermore, Dot5 does not affect redox regulation of the Yap1 transcription factor. These results suggest that Dot5 is less important as an antioxidant in yeast cells.

Authors+Show Affiliations

Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, 611-0011 Kyoto, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12925864

Citation

Izawa, S, et al. "Nuclear Thioredoxin Peroxidase Dot5 in Saccharomyces Cerevisiae: Roles in Oxidative Stress Response and Disruption of Telomeric Silencing." Applied Microbiology and Biotechnology, vol. 64, no. 1, 2004, pp. 120-4.
Izawa S, Kuroki N, Inoue Y. Nuclear thioredoxin peroxidase Dot5 in Saccharomyces cerevisiae: roles in oxidative stress response and disruption of telomeric silencing. Appl Microbiol Biotechnol. 2004;64(1):120-4.
Izawa, S., Kuroki, N., & Inoue, Y. (2004). Nuclear thioredoxin peroxidase Dot5 in Saccharomyces cerevisiae: roles in oxidative stress response and disruption of telomeric silencing. Applied Microbiology and Biotechnology, 64(1), 120-4.
Izawa S, Kuroki N, Inoue Y. Nuclear Thioredoxin Peroxidase Dot5 in Saccharomyces Cerevisiae: Roles in Oxidative Stress Response and Disruption of Telomeric Silencing. Appl Microbiol Biotechnol. 2004;64(1):120-4. PubMed PMID: 12925864.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nuclear thioredoxin peroxidase Dot5 in Saccharomyces cerevisiae: roles in oxidative stress response and disruption of telomeric silencing. AU - Izawa,S, AU - Kuroki,N, AU - Inoue,Y, Y1 - 2003/08/19/ PY - 2003/07/01/received PY - 2003/07/06/revised PY - 2003/07/18/accepted PY - 2003/8/20/pubmed PY - 2004/6/2/medline PY - 2003/8/20/entrez SP - 120 EP - 4 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 64 IS - 1 N2 - The DOT5 gene was originally cloned as one of the DOT (disrupter of telomeric silencing) genes; and later it was re-discovered as a nuclear thioredoxin peroxidase in Saccharomyces cerevisiae. Here, we demonstrate that the telomeric-silencing disruption activity of Dot5 is independent of thioredoxin peroxidase activity. In addition, Dot5 cannot suppress the increased susceptibility to peroxides of mutants defected in cytosolic thioredoxin peroxidase, even when Dot5 is expressed in the cytoplasm. Furthermore, Dot5 does not affect redox regulation of the Yap1 transcription factor. These results suggest that Dot5 is less important as an antioxidant in yeast cells. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/12925864/Nuclear_thioredoxin_peroxidase_Dot5_in_Saccharomyces_cerevisiae:_roles_in_oxidative_stress_response_and_disruption_of_telomeric_silencing_ L2 - https://dx.doi.org/10.1007/s00253-003-1421-5 DB - PRIME DP - Unbound Medicine ER -