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Transgenic mice engineered to target Cre/loxP-mediated DNA recombination into catecholaminergic neurons.
Genesis. 2003 Aug; 36(4):196-202.G

Abstract

To introduce restricted DNA recombination events into catecholaminergic neurons using the Cre/loxP technology, we generated transgenic mice carrying the Cre recombinase gene driven by a 9 kb rat tyrosine hydroxylase (TH) promoter. Immunohistochemistry performed on transgenic mouse brain sections revealed a high number of cells expressing Cre in areas where TH is normally expressed, including the olfactory bulb, hypothalamic and midbrain dopaminergic neurons, and the locus coeruleus. Double immunohistochemistry and immunofluorescence indicated that colocalization of TH and Cre is greater than 80%. Cre expression was also found in TH-positive amacrine neurons of the retina, chromaffin cells of the adrenal medulla, and sympathetic ganglia. We crossbred TH-Cre mice with the floxed reporter strain Z/AP and observed efficient Cre-mediated recombination in all areas expressing TH, indicating that transgenic Cre is functional. Therefore, we have generated a valuable transgenic mouse strain to induce specific mutations of "floxed" genes in catecholaminergic neurons.

Authors+Show Affiliations

Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas and Departmento de Fisiología, Biología Molecular y Celular, Universidad de Buenos Aires, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12929090

Citation

Gelman, Diego M., et al. "Transgenic Mice Engineered to Target Cre/loxP-mediated DNA Recombination Into Catecholaminergic Neurons." Genesis (New York, N.Y. : 2000), vol. 36, no. 4, 2003, pp. 196-202.
Gelman DM, Noaín D, Avale ME, et al. Transgenic mice engineered to target Cre/loxP-mediated DNA recombination into catecholaminergic neurons. Genesis. 2003;36(4):196-202.
Gelman, D. M., Noaín, D., Avale, M. E., Otero, V., Low, M. J., & Rubinstein, M. (2003). Transgenic mice engineered to target Cre/loxP-mediated DNA recombination into catecholaminergic neurons. Genesis (New York, N.Y. : 2000), 36(4), 196-202.
Gelman DM, et al. Transgenic Mice Engineered to Target Cre/loxP-mediated DNA Recombination Into Catecholaminergic Neurons. Genesis. 2003;36(4):196-202. PubMed PMID: 12929090.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transgenic mice engineered to target Cre/loxP-mediated DNA recombination into catecholaminergic neurons. AU - Gelman,Diego M, AU - Noaín,Daniela, AU - Avale,M Elena, AU - Otero,Verónica, AU - Low,Malcolm J, AU - Rubinstein,Marcelo, PY - 2003/8/21/pubmed PY - 2004/4/24/medline PY - 2003/8/21/entrez SP - 196 EP - 202 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 36 IS - 4 N2 - To introduce restricted DNA recombination events into catecholaminergic neurons using the Cre/loxP technology, we generated transgenic mice carrying the Cre recombinase gene driven by a 9 kb rat tyrosine hydroxylase (TH) promoter. Immunohistochemistry performed on transgenic mouse brain sections revealed a high number of cells expressing Cre in areas where TH is normally expressed, including the olfactory bulb, hypothalamic and midbrain dopaminergic neurons, and the locus coeruleus. Double immunohistochemistry and immunofluorescence indicated that colocalization of TH and Cre is greater than 80%. Cre expression was also found in TH-positive amacrine neurons of the retina, chromaffin cells of the adrenal medulla, and sympathetic ganglia. We crossbred TH-Cre mice with the floxed reporter strain Z/AP and observed efficient Cre-mediated recombination in all areas expressing TH, indicating that transgenic Cre is functional. Therefore, we have generated a valuable transgenic mouse strain to induce specific mutations of "floxed" genes in catecholaminergic neurons. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/12929090/Transgenic_mice_engineered_to_target_Cre/loxP_mediated_DNA_recombination_into_catecholaminergic_neurons_ L2 - https://doi.org/10.1002/gene.10217 DB - PRIME DP - Unbound Medicine ER -