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Laboratory diagnosis of two scrub typhus outbreaks at Camp Fuji, Japan in 2000 and 2001 by enzyme-linked immunosorbent assay, rapid flow assay, and Western blot assay using outer membrane 56-kD recombinant proteins.
Am J Trop Med Hyg. 2003 Jul; 69(1):60-6.AJ

Abstract

Two scrub typhus outbreaks occurred among U.S. Marines training at Camp Fuji, Japan, between October 25 and November 3, 2000 and October 17 and November 30, 2001. Nine cases in approximately 800 Marines in 2000 and eight cases in approximately 900 Marines in 2001 (approximate attack rates = 1.1% and 0.9%, respectively) reported with signs and symptoms of fever, rash, headache, lymphadenopathy, myalgia, and eschar. Serologies and rapid response to doxycycline treatment indicated they had scrub typhus. Sixty-four convalescent serum samples (18 suspected cases and 46 negative controls) from U.S. Marines training at Camp Fuji during the outbreaks were assessed by enzyme-linked immunosorbent assay (ELISA), rapid flow assay (RFA), and Western blot assay for evidence of infection with Orientia tsutsugamushi, the causative agent of scrub typhus. All but one suspected case had serologic evidence of scrub typhus and all 46 control sera were non-reactive to O. tsutsugamushi antigens. The recombinant 56-kD antigen (r56) from the Karp, Kato and Gilliam strains of O. tsutsugamushi in an ELISA format provided better results than Karp r56 alone (ELISA and RFA) or whole cell antigen preparation from Karp, Kato and Gilliam (ELISA).

Authors+Show Affiliations

Jiang J
Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, Maryland 20910-7500, USA.
Marienau KJ
No affiliation info available
May LA
No affiliation info available
Beecham HJ
No affiliation info available
Wilkinson R
No affiliation info available
Ching WM
No affiliation info available
Richards AL
No affiliation info available

MeSH

AdultAntigens, BacterialBacterial Outer Membrane ProteinsBlotting, WesternDisease OutbreaksEnzyme-Linked Immunosorbent AssayHumansJapanMaleMilitary PersonnelOrientia tsutsugamushiRecombinant ProteinsScrub TyphusSensitivity and SpecificityTime FactorsUnited States

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12932099

Citation

Jiang, Ju, et al. "Laboratory Diagnosis of Two Scrub Typhus Outbreaks at Camp Fuji, Japan in 2000 and 2001 By Enzyme-linked Immunosorbent Assay, Rapid Flow Assay, and Western Blot Assay Using Outer Membrane 56-kD Recombinant Proteins." The American Journal of Tropical Medicine and Hygiene, vol. 69, no. 1, 2003, pp. 60-6.
Jiang J, Marienau KJ, May LA, et al. Laboratory diagnosis of two scrub typhus outbreaks at Camp Fuji, Japan in 2000 and 2001 by enzyme-linked immunosorbent assay, rapid flow assay, and Western blot assay using outer membrane 56-kD recombinant proteins. Am J Trop Med Hyg. 2003;69(1):60-6.
Jiang, J., Marienau, K. J., May, L. A., Beecham, H. J., Wilkinson, R., Ching, W. M., & Richards, A. L. (2003). Laboratory diagnosis of two scrub typhus outbreaks at Camp Fuji, Japan in 2000 and 2001 by enzyme-linked immunosorbent assay, rapid flow assay, and Western blot assay using outer membrane 56-kD recombinant proteins. The American Journal of Tropical Medicine and Hygiene, 69(1), 60-6.
Jiang J, et al. Laboratory Diagnosis of Two Scrub Typhus Outbreaks at Camp Fuji, Japan in 2000 and 2001 By Enzyme-linked Immunosorbent Assay, Rapid Flow Assay, and Western Blot Assay Using Outer Membrane 56-kD Recombinant Proteins. Am J Trop Med Hyg. 2003;69(1):60-6. PubMed PMID: 12932099.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Laboratory diagnosis of two scrub typhus outbreaks at Camp Fuji, Japan in 2000 and 2001 by enzyme-linked immunosorbent assay, rapid flow assay, and Western blot assay using outer membrane 56-kD recombinant proteins. AU - Jiang,Ju, AU - Marienau,Karen J, AU - May,Laurel A, AU - Beecham,H James,3rd AU - Wilkinson,Ray, AU - Ching,Wei-Mei, AU - Richards,Allen L, PY - 2003/8/23/pubmed PY - 2003/9/10/medline PY - 2003/8/23/entrez SP - 60 EP - 6 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 69 IS - 1 N2 - Two scrub typhus outbreaks occurred among U.S. Marines training at Camp Fuji, Japan, between October 25 and November 3, 2000 and October 17 and November 30, 2001. Nine cases in approximately 800 Marines in 2000 and eight cases in approximately 900 Marines in 2001 (approximate attack rates = 1.1% and 0.9%, respectively) reported with signs and symptoms of fever, rash, headache, lymphadenopathy, myalgia, and eschar. Serologies and rapid response to doxycycline treatment indicated they had scrub typhus. Sixty-four convalescent serum samples (18 suspected cases and 46 negative controls) from U.S. Marines training at Camp Fuji during the outbreaks were assessed by enzyme-linked immunosorbent assay (ELISA), rapid flow assay (RFA), and Western blot assay for evidence of infection with Orientia tsutsugamushi, the causative agent of scrub typhus. All but one suspected case had serologic evidence of scrub typhus and all 46 control sera were non-reactive to O. tsutsugamushi antigens. The recombinant 56-kD antigen (r56) from the Karp, Kato and Gilliam strains of O. tsutsugamushi in an ELISA format provided better results than Karp r56 alone (ELISA and RFA) or whole cell antigen preparation from Karp, Kato and Gilliam (ELISA). SN - 0002-9637 UR - https://www.unboundmedicine.com/medline/citation/12932099/Laboratory_diagnosis_of_two_scrub_typhus_outbreaks_at_Camp_Fuji_Japan_in_2000_and_2001_by_enzyme_linked_immunosorbent_assay_rapid_flow_assay_and_Western_blot_assay_using_outer_membrane_56_kD_recombinant_proteins_ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓27]

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