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Methylobacterium sp. isolated from a Finnish paper machine produces highly pyruvated galactan exopolysaccharide.
Carbohydr Res. 2003 Sep 01; 338(18):1851-9.CR

Abstract

The slime-forming bacterium Methylobacterium sp. was isolated from a Finnish paper machine and its exopolysaccharide (EPS) was produced on laboratory scale. Sugar compositional analysis revealed a 100% galactan (EPS). However, FT-IR showed a very strong peak at 1611 cm(-1) showing the presence of pyruvate. Analysis of the pyruvate content revealed that, based on the sugar composition, the EPS consists of a trisaccharide repeating unit consisting of D-galactopyranose and [4,6-O-(1-carboxyethylidene)]-D-galactopyranose with a molar ratio of 1:2, respectively. Both linkage analysis and 2D homo- and heteronuclear 1H and 13C NMR spectroscopy revealed the following repeating unit: -->3)-[4,6-O-(1-carboxyethylidene)]-alpha-D-Galp-(1-->3)[4,6-O-(1-carboxyethylidene)]-alpha-D-Galp-(1-->3)-alpha-D-Galp-(1-->. By enrichment cultures from various ground and compost heap samples a polysaccharide-degrading culture was obtained that produced an endo acting enzyme able to degrade the EPS described. The enzyme hydrolysed the EPS to a large extent, releasing oligomers that mainly consisted out of two repeating units.

Authors+Show Affiliations

Department of Agrotechnology and Food Sciences, Laboratory of Food Chemistry, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12932368

Citation

Verhoef, René, et al. "Methylobacterium Sp. Isolated From a Finnish Paper Machine Produces Highly Pyruvated Galactan Exopolysaccharide." Carbohydrate Research, vol. 338, no. 18, 2003, pp. 1851-9.
Verhoef R, de Waard P, Schols HA, et al. Methylobacterium sp. isolated from a Finnish paper machine produces highly pyruvated galactan exopolysaccharide. Carbohydr Res. 2003;338(18):1851-9.
Verhoef, R., de Waard, P., Schols, H. A., Siika-aho, M., & Voragen, A. G. (2003). Methylobacterium sp. isolated from a Finnish paper machine produces highly pyruvated galactan exopolysaccharide. Carbohydrate Research, 338(18), 1851-9.
Verhoef R, et al. Methylobacterium Sp. Isolated From a Finnish Paper Machine Produces Highly Pyruvated Galactan Exopolysaccharide. Carbohydr Res. 2003 Sep 1;338(18):1851-9. PubMed PMID: 12932368.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Methylobacterium sp. isolated from a Finnish paper machine produces highly pyruvated galactan exopolysaccharide. AU - Verhoef,René, AU - de Waard,Pieter, AU - Schols,Henk A, AU - Siika-aho,Matti, AU - Voragen,Alphons G J, PY - 2003/8/23/pubmed PY - 2004/6/25/medline PY - 2003/8/23/entrez SP - 1851 EP - 9 JF - Carbohydrate research JO - Carbohydr Res VL - 338 IS - 18 N2 - The slime-forming bacterium Methylobacterium sp. was isolated from a Finnish paper machine and its exopolysaccharide (EPS) was produced on laboratory scale. Sugar compositional analysis revealed a 100% galactan (EPS). However, FT-IR showed a very strong peak at 1611 cm(-1) showing the presence of pyruvate. Analysis of the pyruvate content revealed that, based on the sugar composition, the EPS consists of a trisaccharide repeating unit consisting of D-galactopyranose and [4,6-O-(1-carboxyethylidene)]-D-galactopyranose with a molar ratio of 1:2, respectively. Both linkage analysis and 2D homo- and heteronuclear 1H and 13C NMR spectroscopy revealed the following repeating unit: -->3)-[4,6-O-(1-carboxyethylidene)]-alpha-D-Galp-(1-->3)[4,6-O-(1-carboxyethylidene)]-alpha-D-Galp-(1-->3)-alpha-D-Galp-(1-->. By enrichment cultures from various ground and compost heap samples a polysaccharide-degrading culture was obtained that produced an endo acting enzyme able to degrade the EPS described. The enzyme hydrolysed the EPS to a large extent, releasing oligomers that mainly consisted out of two repeating units. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/12932368/Methylobacterium_sp__isolated_from_a_Finnish_paper_machine_produces_highly_pyruvated_galactan_exopolysaccharide_ DB - PRIME DP - Unbound Medicine ER -