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Depletion of SecDF-YajC causes a decrease in the level of SecG: implication for their functional interaction.
FEBS Lett 2003; 550(1-3):114-8FL

Abstract

SecA and an apparatus comprising SecYEG and SecDF-YajC complexes catalyze protein translocation across the Escherichia coli membrane. SecDF-YajC and SecG facilitate membrane insertion of SecA, which is the driving force for protein translocation. Here we report that SecDF-YajC depletion together with SecG depletion nearly completely inhibits protein translocation both in vivo and in vitro, although SecDF-YajC had been thought to be unnecessary for in vitro translocation. The level of SecG in membranes decreased to about half upon SecDF-YajC depletion and recovered to a normal level when SecDF-YajC was expressed. SecDF-YajC inhibited disulfide bond formation between two SecG molecules possessing a single cysteine residue. These results suggest functional interaction between SecDF-YajC and SecG.

Authors+Show Affiliations

Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12935896

Citation

Kato, Yoshihisa, et al. "Depletion of SecDF-YajC Causes a Decrease in the Level of SecG: Implication for Their Functional Interaction." FEBS Letters, vol. 550, no. 1-3, 2003, pp. 114-8.
Kato Y, Nishiyama K, Tokuda H. Depletion of SecDF-YajC causes a decrease in the level of SecG: implication for their functional interaction. FEBS Lett. 2003;550(1-3):114-8.
Kato, Y., Nishiyama, K., & Tokuda, H. (2003). Depletion of SecDF-YajC causes a decrease in the level of SecG: implication for their functional interaction. FEBS Letters, 550(1-3), pp. 114-8.
Kato Y, Nishiyama K, Tokuda H. Depletion of SecDF-YajC Causes a Decrease in the Level of SecG: Implication for Their Functional Interaction. FEBS Lett. 2003 Aug 28;550(1-3):114-8. PubMed PMID: 12935896.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Depletion of SecDF-YajC causes a decrease in the level of SecG: implication for their functional interaction. AU - Kato,Yoshihisa, AU - Nishiyama,Ken-ichi, AU - Tokuda,Hajime, PY - 2003/8/26/pubmed PY - 2003/11/13/medline PY - 2003/8/26/entrez SP - 114 EP - 8 JF - FEBS letters JO - FEBS Lett. VL - 550 IS - 1-3 N2 - SecA and an apparatus comprising SecYEG and SecDF-YajC complexes catalyze protein translocation across the Escherichia coli membrane. SecDF-YajC and SecG facilitate membrane insertion of SecA, which is the driving force for protein translocation. Here we report that SecDF-YajC depletion together with SecG depletion nearly completely inhibits protein translocation both in vivo and in vitro, although SecDF-YajC had been thought to be unnecessary for in vitro translocation. The level of SecG in membranes decreased to about half upon SecDF-YajC depletion and recovered to a normal level when SecDF-YajC was expressed. SecDF-YajC inhibited disulfide bond formation between two SecG molecules possessing a single cysteine residue. These results suggest functional interaction between SecDF-YajC and SecG. SN - 0014-5793 UR - https://www.unboundmedicine.com/medline/citation/12935896/Depletion_of_SecDF_YajC_causes_a_decrease_in_the_level_of_SecG:_implication_for_their_functional_interaction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014579303008470 DB - PRIME DP - Unbound Medicine ER -