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Leukemia markers expression of peripheral blood vs bone marrow blasts using flow cytometry.
Med Sci Monit. 2003 Aug; 9(8):CR359-62.MS

Abstract

BACKGROUND

Flow cytometric techniques are widely used in clinical hematology. Characterization of leukemias by immunotyping is particularly helpful when the morphology is difficult to interpret. The major advantage of using immune markers by flow cytometry is the identification of particular leukemia subtype, not recognized by morphologic criteria, which may have prognostic significance. Current literature suggests when peripheral blood (PB) is consisted of 30% blasts or higher diagnosis of acute leukemia is most likely. However, bone marrow aspiration may also be performed as a confirmatory diagnosis. Immunotyping of PB and BM in leukemias not only determine the decision making for a specific therapeutic regimen, but also is a practical prognostic indicator.

MATERIAL/METHODS

We evaluated 18 patients with acute myeloid Leukemia (AML) and 13 patients with acute lymphoid leukemia (ALL). In all cases, the amount of blasts in PB was 30% or higher. Two ml PB and BM samples from each patient was collected. Following the preparation process, expression of markers was detected by using flow cytometry. The panel of monoclonal antibodies used in this study were consisted of CD3, CD7, CD5 (for T lymphocytes lineage); CD19, CD22, CD20, CD10 (for B lymphocytes lineage); CD13, CD14, CD33 (for myeloid subsets); and TDT, HLA-DR, CD45 (non lineage restricted). Expression levels of PB and BM markers were compared by using statistical analysis.

RESULTS

The results showed apparent discrepancies for some markers in ALL group. However, in AML patients most of the selected markers have shown considerable correlation between PB and BM samples. Only four markers (CD13, CD14, CD45, and HLA-DR) showed positive correlation. In contrast, most markers (CD3, CD5, CD13, CD14, CD19, CD45, HLA-DR, and TdT) showed strong correlation between PB and BM samples in AML group.

CONCLUSIONS

The findings of this study suggests that targeted gating strategy for blast population as well as selection of a suitable panel of monoclonal antibodies may be essential for diagnosis of leukemia resulting in similar immunotyping pattern in PB and BM. Although our results are preliminary, this can minimize the necessity of BM aspiration for leukemia patients.

Authors+Show Affiliations

Depertment of Immunology, School of Medicine, University of Medical Sciences, Isfahan, Iran. rezaei@mui.ac.irNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12942032

Citation

Rezaei, Abbas, et al. "Leukemia Markers Expression of Peripheral Blood Vs Bone Marrow Blasts Using Flow Cytometry." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 9, no. 8, 2003, pp. CR359-62.
Rezaei A, Adib M, Mokarian F, et al. Leukemia markers expression of peripheral blood vs bone marrow blasts using flow cytometry. Med Sci Monit. 2003;9(8):CR359-62.
Rezaei, A., Adib, M., Mokarian, F., Tebianian, M., & Nassiri, R. (2003). Leukemia markers expression of peripheral blood vs bone marrow blasts using flow cytometry. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 9(8), CR359-62.
Rezaei A, et al. Leukemia Markers Expression of Peripheral Blood Vs Bone Marrow Blasts Using Flow Cytometry. Med Sci Monit. 2003;9(8):CR359-62. PubMed PMID: 12942032.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leukemia markers expression of peripheral blood vs bone marrow blasts using flow cytometry. AU - Rezaei,Abbas, AU - Adib,Minoo, AU - Mokarian,Fariborz, AU - Tebianian,Majid, AU - Nassiri,Reza, PY - 2003/8/28/pubmed PY - 2004/2/11/medline PY - 2003/8/28/entrez SP - CR359 EP - 62 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med Sci Monit VL - 9 IS - 8 N2 - BACKGROUND: Flow cytometric techniques are widely used in clinical hematology. Characterization of leukemias by immunotyping is particularly helpful when the morphology is difficult to interpret. The major advantage of using immune markers by flow cytometry is the identification of particular leukemia subtype, not recognized by morphologic criteria, which may have prognostic significance. Current literature suggests when peripheral blood (PB) is consisted of 30% blasts or higher diagnosis of acute leukemia is most likely. However, bone marrow aspiration may also be performed as a confirmatory diagnosis. Immunotyping of PB and BM in leukemias not only determine the decision making for a specific therapeutic regimen, but also is a practical prognostic indicator. MATERIAL/METHODS: We evaluated 18 patients with acute myeloid Leukemia (AML) and 13 patients with acute lymphoid leukemia (ALL). In all cases, the amount of blasts in PB was 30% or higher. Two ml PB and BM samples from each patient was collected. Following the preparation process, expression of markers was detected by using flow cytometry. The panel of monoclonal antibodies used in this study were consisted of CD3, CD7, CD5 (for T lymphocytes lineage); CD19, CD22, CD20, CD10 (for B lymphocytes lineage); CD13, CD14, CD33 (for myeloid subsets); and TDT, HLA-DR, CD45 (non lineage restricted). Expression levels of PB and BM markers were compared by using statistical analysis. RESULTS: The results showed apparent discrepancies for some markers in ALL group. However, in AML patients most of the selected markers have shown considerable correlation between PB and BM samples. Only four markers (CD13, CD14, CD45, and HLA-DR) showed positive correlation. In contrast, most markers (CD3, CD5, CD13, CD14, CD19, CD45, HLA-DR, and TdT) showed strong correlation between PB and BM samples in AML group. CONCLUSIONS: The findings of this study suggests that targeted gating strategy for blast population as well as selection of a suitable panel of monoclonal antibodies may be essential for diagnosis of leukemia resulting in similar immunotyping pattern in PB and BM. Although our results are preliminary, this can minimize the necessity of BM aspiration for leukemia patients. SN - 1234-1010 UR - https://www.unboundmedicine.com/medline/citation/12942032/Leukemia_markers_expression_of_peripheral_blood_vs_bone_marrow_blasts_using_flow_cytometry_ L2 - https://www.medscimonit.com/download/index/idArt/13099 DB - PRIME DP - Unbound Medicine ER -