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[Effect of new peptide bioregulators livagen and epitalon on enkephalin-degrading enzymes in human serum].
Izv Akad Nauk Ser Biol. 2003 Jul-AugIA

Abstract

The effect of new peptide bioregulators--Livagen (Lys-Glu-Asp-Ala) and Epitalon (Ala-Glu-Asp-Gly)--on endogenous opioid system was studied, particularly, their ability to change the activity of enkephalin-degrading enzymes from serum and interact with opioid receptors of the brain membrane fraction. Enkephalinase activity was assayed in vitro by the rate of 3H-Leu-enkephalin hydrolysis in the presence of the tested peptides. Livagen and Epitalon inhibited enkephalin-degrading enzymes from human serum. Livagen proved to be more efficient also as compared to well-known peptidase inhibitors such as puromycin, leupeptin, and D-PAM. The dose-inhibitory effect curves for Livagen and Epitalon were plotted; their IC50 equaled 20 and 500 microM, respectively. The interaction between the peptides and opioid receptors was estimated using a radioreceptor method with [3H][D-Ala2, D-Leu5]-enkephalin. No interaction was observed between the tested peptides and mu- or delta-opioid receptors of the membrane fraction from the rat brain.

Authors+Show Affiliations

Kost NV
Mental Health Research Center, Russian Academy of Medical Sciences, Kashirskoe sh. 34, Moscow, 115522 Russia.
Sokolov OIu
No affiliation info available
Gabaeva MV
No affiliation info available
Zolotarev IuA
No affiliation info available
Malinin VV
No affiliation info available
Khavinson VKh
No affiliation info available

MeSH

AnimalsBrainEnkephalin, LeucineEnzyme InhibitorsEnzymesHumansIn Vitro TechniquesNeprilysinOligonucleotidesOligopeptidesRatsRats, WistarReceptors, Opioid, deltaReceptors, Opioid, mu

Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

12942748

Citation

Kost, N V., et al. "[Effect of New Peptide Bioregulators Livagen and Epitalon On Enkephalin-degrading Enzymes in Human Serum]." Izvestiia Akademii Nauk. Seriia Biologicheskaia, 2003, pp. 427-9.
Kost NV, Sokolov OIu, Gabaeva MV, et al. [Effect of new peptide bioregulators livagen and epitalon on enkephalin-degrading enzymes in human serum]. Izv Akad Nauk Ser Biol. 2003.
Kost, N. V., Sokolov, O. I. u., Gabaeva, M. V., Zolotarev, I. u. A., Malinin, V. V., & Khavinson, V. K. h. (2003). [Effect of new peptide bioregulators livagen and epitalon on enkephalin-degrading enzymes in human serum]. Izvestiia Akademii Nauk. Seriia Biologicheskaia, (4), 427-9.
Kost NV, et al. [Effect of New Peptide Bioregulators Livagen and Epitalon On Enkephalin-degrading Enzymes in Human Serum]. Izv Akad Nauk Ser Biol. 2003 Jul-Aug;(4)427-9. PubMed PMID: 12942748.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of new peptide bioregulators livagen and epitalon on enkephalin-degrading enzymes in human serum]. AU - Kost,N V, AU - Sokolov,O Iu, AU - Gabaeva,M V, AU - Zolotarev,Iu A, AU - Malinin,V V, AU - Khavinson,V Kh, PY - 2003/8/29/pubmed PY - 2003/10/16/medline PY - 2003/8/29/entrez SP - 427 EP - 9 JF - Izvestiia Akademii nauk. Seriia biologicheskaia JO - Izv Akad Nauk Ser Biol IS - 4 N2 - The effect of new peptide bioregulators--Livagen (Lys-Glu-Asp-Ala) and Epitalon (Ala-Glu-Asp-Gly)--on endogenous opioid system was studied, particularly, their ability to change the activity of enkephalin-degrading enzymes from serum and interact with opioid receptors of the brain membrane fraction. Enkephalinase activity was assayed in vitro by the rate of 3H-Leu-enkephalin hydrolysis in the presence of the tested peptides. Livagen and Epitalon inhibited enkephalin-degrading enzymes from human serum. Livagen proved to be more efficient also as compared to well-known peptidase inhibitors such as puromycin, leupeptin, and D-PAM. The dose-inhibitory effect curves for Livagen and Epitalon were plotted; their IC50 equaled 20 and 500 microM, respectively. The interaction between the peptides and opioid receptors was estimated using a radioreceptor method with [3H][D-Ala2, D-Leu5]-enkephalin. No interaction was observed between the tested peptides and mu- or delta-opioid receptors of the membrane fraction from the rat brain. SN - 1026-3470 UR - https://www.unboundmedicine.com/medline/citation/12942748/[Effect_of_new_peptide_bioregulators_livagen_and_epitalon_on_enkephalin_degrading_enzymes_in_human_serum]_ DB - PRIME DP - Unbound Medicine ER -