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A comprehensive haplotype analysis of CYP19 and breast cancer risk: the Multiethnic Cohort.
Hum Mol Genet. 2003 Oct 15; 12(20):2679-92.HM

Abstract

The CYP19 gene encodes for aromatase (P450arom), a key steroidogenic enzyme that catalyzes the final step of estrogen biosynthesis. Apart from rare mutations in CYP19 which result in severe phenotypes associated with estrogen insufficiency, little is known about whether common variation in CYP19 is associated with risk of hormone-related diseases. In this study, we employed a haplotype-based approach to search for common disease-associated variants in this candidate breast cancer susceptibility gene among African-American, Hawaiian, Japanese, Latina and White women in the Multiethnic Cohort Study (MEC). We utilized 74 densely spaced single-nucleotide polymorphisms (SNPs) (one every approximately 2.6 kb) spanning 189.4 kb of the CYP19 locus to characterize linkage disequilibrium (LD) and haplotype patterns among 69-70 individuals from each ethnic population. We detected four regions of strong LD (blocks 1-4) that were quite closely conserved across populations. Within each block there was a limited diversity of common haplotypes (5 to 10 with a frequency >/=5%) and most haplotypes were observed to be shared across populations. Twenty-five haplotype-tagging SNPs (htSNPs) were selected to predict the common haplotypes with high probability (average Rh2=0.92) and genotyped in a breast cancer case-control study in the MEC (cases, n=1355; controls, n=2580). We first performed global tests for differences in risk according to the common haplotypes and observed significant haplotype-effects in block 2 [P=0.01; haplotypes 2b (OR=1.23; 95% CI, 1.07-1.40), 2d (OR=1.28; 95% CI, 1.01-1.62)]. We also found a common long-range haplotype comprised of block-specific haplotypes 2b and 3c to be associated with increased risk of breast cancer (haplotype 2b-3c: OR=1.31; 95% CI, 1.11-1.54). Our findings suggest the hypothesis that women with the long-range CYP19 haplotype 2b-3c may be carriers of a predisposing breast cancer susceptibility allele.

Authors+Show Affiliations

Department of Preventive Medicine, Keck School of Medicine, University of Southern California, USC/Norris Comprehensive Cancer Center, 1441 Eastlake Ave, Rm 4441, Los Angeles, CA 90089-9175, USA. haiman@usc.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12944421

Citation

Haiman, Christopher A., et al. "A Comprehensive Haplotype Analysis of CYP19 and Breast Cancer Risk: the Multiethnic Cohort." Human Molecular Genetics, vol. 12, no. 20, 2003, pp. 2679-92.
Haiman CA, Stram DO, Pike MC, et al. A comprehensive haplotype analysis of CYP19 and breast cancer risk: the Multiethnic Cohort. Hum Mol Genet. 2003;12(20):2679-92.
Haiman, C. A., Stram, D. O., Pike, M. C., Kolonel, L. N., Burtt, N. P., Altshuler, D., Hirschhorn, J., & Henderson, B. E. (2003). A comprehensive haplotype analysis of CYP19 and breast cancer risk: the Multiethnic Cohort. Human Molecular Genetics, 12(20), 2679-92.
Haiman CA, et al. A Comprehensive Haplotype Analysis of CYP19 and Breast Cancer Risk: the Multiethnic Cohort. Hum Mol Genet. 2003 Oct 15;12(20):2679-92. PubMed PMID: 12944421.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comprehensive haplotype analysis of CYP19 and breast cancer risk: the Multiethnic Cohort. AU - Haiman,Christopher A, AU - Stram,Daniel O, AU - Pike,Malcolm C, AU - Kolonel,Laurence N, AU - Burtt,Noel P, AU - Altshuler,David, AU - Hirschhorn,Joel, AU - Henderson,Brian E, Y1 - 2003/08/27/ PY - 2003/8/29/pubmed PY - 2004/7/21/medline PY - 2003/8/29/entrez SP - 2679 EP - 92 JF - Human molecular genetics JO - Hum Mol Genet VL - 12 IS - 20 N2 - The CYP19 gene encodes for aromatase (P450arom), a key steroidogenic enzyme that catalyzes the final step of estrogen biosynthesis. Apart from rare mutations in CYP19 which result in severe phenotypes associated with estrogen insufficiency, little is known about whether common variation in CYP19 is associated with risk of hormone-related diseases. In this study, we employed a haplotype-based approach to search for common disease-associated variants in this candidate breast cancer susceptibility gene among African-American, Hawaiian, Japanese, Latina and White women in the Multiethnic Cohort Study (MEC). We utilized 74 densely spaced single-nucleotide polymorphisms (SNPs) (one every approximately 2.6 kb) spanning 189.4 kb of the CYP19 locus to characterize linkage disequilibrium (LD) and haplotype patterns among 69-70 individuals from each ethnic population. We detected four regions of strong LD (blocks 1-4) that were quite closely conserved across populations. Within each block there was a limited diversity of common haplotypes (5 to 10 with a frequency >/=5%) and most haplotypes were observed to be shared across populations. Twenty-five haplotype-tagging SNPs (htSNPs) were selected to predict the common haplotypes with high probability (average Rh2=0.92) and genotyped in a breast cancer case-control study in the MEC (cases, n=1355; controls, n=2580). We first performed global tests for differences in risk according to the common haplotypes and observed significant haplotype-effects in block 2 [P=0.01; haplotypes 2b (OR=1.23; 95% CI, 1.07-1.40), 2d (OR=1.28; 95% CI, 1.01-1.62)]. We also found a common long-range haplotype comprised of block-specific haplotypes 2b and 3c to be associated with increased risk of breast cancer (haplotype 2b-3c: OR=1.31; 95% CI, 1.11-1.54). Our findings suggest the hypothesis that women with the long-range CYP19 haplotype 2b-3c may be carriers of a predisposing breast cancer susceptibility allele. SN - 0964-6906 UR - https://www.unboundmedicine.com/medline/citation/12944421/A_comprehensive_haplotype_analysis_of_CYP19_and_breast_cancer_risk:_the_Multiethnic_Cohort_ L2 - https://academic.oup.com/hmg/article-lookup/doi/10.1093/hmg/ddg294 DB - PRIME DP - Unbound Medicine ER -