Tags

Type your tag names separated by a space and hit enter

Molecular mechanism of citalopram and cocaine interactions with neurotransmitter transporters.
J Pharmacol Exp Ther. 2003 Oct; 307(1):34-41.JP

Abstract

The selective serotonin reuptake inhibitors (SSRIs) and cocaine bind to the neural serotonin (5-HT) transporter (SERT) and thus inhibit presynaptic reuptake of 5-HT and elevate its concentration in the synaptic cleft. Cocaine also binds to the dopamine transporter (DAT) and to the noradrenaline transporter (NET) and inhibits presynaptic reuptake of dopamine and noradrenaline. SERT, DAT, and NET belong to the sodium/neurotransmitter symporter family, which is predicted to have a molecular structure with 12 transmembrane alpha-helices (TMHs) and intracellular amino- and carboxy terminals. We used an electron density projection map of the Escherichia coli Na+/H+ anti-porter, and site-directed mutagenesis data on DAT and SERT to construct 3-dimensional molecular models of SERT, DAT and NET. These models were used to simulate the molecular interaction mechanisms of the SSRI, S-citalopram, its less potent enantiomer, R-citalopram and of cocaine with the transporters. In the SERT model, a single amino acid (Tyr95) in TMH1 determined the transporter selectivity of S-citalopram for SERT over DAT and NET. A dipole-dipole interaction was formed between the hydroxy group of Tyr95 in SERT and the nitril group of S-citalopram, but could not be formed by S-citalopram in DAT and NET where the corresponding amino acid is a phenylalanine. The lower binding affinity of R-citalopram may be due to sterical hindrance at the binding site. The tropane ring of cocaine interacted with Tyr95 in SERT and with the corresponding phenylalanines in NET and DAT. This may explain why cocaine, but not S-citalopram, has high binding affinity to all three transporters.

Authors+Show Affiliations

Department of Pharmacology, Institute of Medical Biology, University of Tromsø, Norway.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12944499

Citation

Ravna, Aina Westrheim, et al. "Molecular Mechanism of Citalopram and Cocaine Interactions With Neurotransmitter Transporters." The Journal of Pharmacology and Experimental Therapeutics, vol. 307, no. 1, 2003, pp. 34-41.
Ravna AW, Sylte I, Dahl SG. Molecular mechanism of citalopram and cocaine interactions with neurotransmitter transporters. J Pharmacol Exp Ther. 2003;307(1):34-41.
Ravna, A. W., Sylte, I., & Dahl, S. G. (2003). Molecular mechanism of citalopram and cocaine interactions with neurotransmitter transporters. The Journal of Pharmacology and Experimental Therapeutics, 307(1), 34-41.
Ravna AW, Sylte I, Dahl SG. Molecular Mechanism of Citalopram and Cocaine Interactions With Neurotransmitter Transporters. J Pharmacol Exp Ther. 2003;307(1):34-41. PubMed PMID: 12944499.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular mechanism of citalopram and cocaine interactions with neurotransmitter transporters. AU - Ravna,Aina Westrheim, AU - Sylte,Ingebrigt, AU - Dahl,Svein G, Y1 - 2003/08/27/ PY - 2003/8/29/pubmed PY - 2003/10/24/medline PY - 2003/8/29/entrez SP - 34 EP - 41 JF - The Journal of pharmacology and experimental therapeutics JO - J Pharmacol Exp Ther VL - 307 IS - 1 N2 - The selective serotonin reuptake inhibitors (SSRIs) and cocaine bind to the neural serotonin (5-HT) transporter (SERT) and thus inhibit presynaptic reuptake of 5-HT and elevate its concentration in the synaptic cleft. Cocaine also binds to the dopamine transporter (DAT) and to the noradrenaline transporter (NET) and inhibits presynaptic reuptake of dopamine and noradrenaline. SERT, DAT, and NET belong to the sodium/neurotransmitter symporter family, which is predicted to have a molecular structure with 12 transmembrane alpha-helices (TMHs) and intracellular amino- and carboxy terminals. We used an electron density projection map of the Escherichia coli Na+/H+ anti-porter, and site-directed mutagenesis data on DAT and SERT to construct 3-dimensional molecular models of SERT, DAT and NET. These models were used to simulate the molecular interaction mechanisms of the SSRI, S-citalopram, its less potent enantiomer, R-citalopram and of cocaine with the transporters. In the SERT model, a single amino acid (Tyr95) in TMH1 determined the transporter selectivity of S-citalopram for SERT over DAT and NET. A dipole-dipole interaction was formed between the hydroxy group of Tyr95 in SERT and the nitril group of S-citalopram, but could not be formed by S-citalopram in DAT and NET where the corresponding amino acid is a phenylalanine. The lower binding affinity of R-citalopram may be due to sterical hindrance at the binding site. The tropane ring of cocaine interacted with Tyr95 in SERT and with the corresponding phenylalanines in NET and DAT. This may explain why cocaine, but not S-citalopram, has high binding affinity to all three transporters. SN - 0022-3565 UR - https://www.unboundmedicine.com/medline/citation/12944499/Molecular_mechanism_of_citalopram_and_cocaine_interactions_with_neurotransmitter_transporters_ L2 - https://jpet.aspetjournals.org/cgi/pmidlookup?view=long&pmid=12944499 DB - PRIME DP - Unbound Medicine ER -