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Detection of IgM specific antibody using indirect immunofluorescent assay for diagnosis of acute leptospirosis.
J Med Assoc Thai. 2003 Jul; 86(7):641-6.JM

Abstract

Leptospirosis is a worldwide zoonosis, caused by Leptospira interrogans. At the earlier stage of the disease, the IgM immunoassays are expected to have more sensitivity than other immunoassays. Previous reports showed that the indirect immunofluorescent assay for detection of immunoglobulins (IFA-Igs) against Leptospira spp showed higher sensitive and specific than some genus specific tests. The authors determined an efficacy of the indirect immunofluorescent assay for detection of IgM specific leptospiral antibody (IFA-IgM). One hundred and eighty patients with acute febrile illness without localizing signs admitted to Hat Yai Hospital were studied. Using the cut-off value of > or = 1:400, the overall sensitivity, and overall specificity of the IFA-IgM were 89.2 per cent and 95.1 per cent, respectively. They were slightly greater than those of the conventional IFA-Igs (86.5% and 91.6%). The first sera obtained from patients including acute sera and single sera showed a low sensitivity (32.4%) but still higher than the IFA-Igs (29.7%). None of the patients with various diseases commonly confused with leptospirosis or healthy blood donors gave a titer greater than 1:200. In conclusion, the IFA-IgM has the same protocol as the IFA-Igs but the sensitivity and specificity is slightly greater than the IFA-Igs. This is another alternative test for the diagnosis of acute leptospirosis.

Authors+Show Affiliations

Department of Pathology, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, Thailand.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12948259

Citation

Pradutkanchana, S, et al. "Detection of IgM Specific Antibody Using Indirect Immunofluorescent Assay for Diagnosis of Acute Leptospirosis." Journal of the Medical Association of Thailand = Chotmaihet Thangphaet, vol. 86, no. 7, 2003, pp. 641-6.
Pradutkanchana S, Pradutkanchana J, Khuntikij P. Detection of IgM specific antibody using indirect immunofluorescent assay for diagnosis of acute leptospirosis. J Med Assoc Thai. 2003;86(7):641-6.
Pradutkanchana, S., Pradutkanchana, J., & Khuntikij, P. (2003). Detection of IgM specific antibody using indirect immunofluorescent assay for diagnosis of acute leptospirosis. Journal of the Medical Association of Thailand = Chotmaihet Thangphaet, 86(7), 641-6.
Pradutkanchana S, Pradutkanchana J, Khuntikij P. Detection of IgM Specific Antibody Using Indirect Immunofluorescent Assay for Diagnosis of Acute Leptospirosis. J Med Assoc Thai. 2003;86(7):641-6. PubMed PMID: 12948259.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of IgM specific antibody using indirect immunofluorescent assay for diagnosis of acute leptospirosis. AU - Pradutkanchana,S, AU - Pradutkanchana,J, AU - Khuntikij,P, PY - 2003/9/2/pubmed PY - 2003/10/3/medline PY - 2003/9/2/entrez SP - 641 EP - 6 JF - Journal of the Medical Association of Thailand = Chotmaihet thangphaet JO - J Med Assoc Thai VL - 86 IS - 7 N2 - Leptospirosis is a worldwide zoonosis, caused by Leptospira interrogans. At the earlier stage of the disease, the IgM immunoassays are expected to have more sensitivity than other immunoassays. Previous reports showed that the indirect immunofluorescent assay for detection of immunoglobulins (IFA-Igs) against Leptospira spp showed higher sensitive and specific than some genus specific tests. The authors determined an efficacy of the indirect immunofluorescent assay for detection of IgM specific leptospiral antibody (IFA-IgM). One hundred and eighty patients with acute febrile illness without localizing signs admitted to Hat Yai Hospital were studied. Using the cut-off value of > or = 1:400, the overall sensitivity, and overall specificity of the IFA-IgM were 89.2 per cent and 95.1 per cent, respectively. They were slightly greater than those of the conventional IFA-Igs (86.5% and 91.6%). The first sera obtained from patients including acute sera and single sera showed a low sensitivity (32.4%) but still higher than the IFA-Igs (29.7%). None of the patients with various diseases commonly confused with leptospirosis or healthy blood donors gave a titer greater than 1:200. In conclusion, the IFA-IgM has the same protocol as the IFA-Igs but the sensitivity and specificity is slightly greater than the IFA-Igs. This is another alternative test for the diagnosis of acute leptospirosis. SN - 0125-2208 UR - https://www.unboundmedicine.com/medline/citation/12948259/Detection_of_IgM_specific_antibody_using_indirect_immunofluorescent_assay_for_diagnosis_of_acute_leptospirosis_ L2 - https://www.diseaseinfosearch.org/result/4178 DB - PRIME DP - Unbound Medicine ER -