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SDF-1 increases recruitment of osteoclast precursors by upregulation of matrix metalloproteinase-9 activity.
Connect Tissue Res. 2003; 44 Suppl 1:79-84.CT

Abstract

Although chemokines play essential roles in the trafficking and homing of many circulating hematopoietic cell types, their potential influences on osteoclast (OC) recruitment or bone remodeling are not well known. Therefore, chemokine receptor expression was analyzed by RNase protection assay during OC formation induced by RANKL in a murine mononuclear cell line (RAW 264.7). Relatively high CXCR4 expression was detected in RAW cells (pre-OCs), whereas CXCR4 levels were downregulated during RAW-OC development. SDF-1, the unique ligand for CXCR4, stimulated RAW cell production of matrix metalloproteinase (MMP)-9 activity, a matrix-degrading enzyme essential for pre-OC migration into the developing bone marrow cavity. Induced MMP-9 activity in RAW cells was associated with their increased MMP-dependent transmigration through a collagen gel in response to SDF-1. We conclude that SDF-1 stimulation of MMP-9 activity in pre-OCs may be a key aspect of their recruitment to bone and migration within the marrow to sites for OC differentiation and bone resorption.

Authors+Show Affiliations

Department of Biology, Washington University, St. Louis, Missouri 63130, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12952178

Citation

Yu, Xuefeng, et al. "SDF-1 Increases Recruitment of Osteoclast Precursors By Upregulation of Matrix Metalloproteinase-9 Activity." Connective Tissue Research, vol. 44 Suppl 1, 2003, pp. 79-84.
Yu X, Collin-Osdoby P, Osdoby P. SDF-1 increases recruitment of osteoclast precursors by upregulation of matrix metalloproteinase-9 activity. Connect Tissue Res. 2003;44 Suppl 1:79-84.
Yu, X., Collin-Osdoby, P., & Osdoby, P. (2003). SDF-1 increases recruitment of osteoclast precursors by upregulation of matrix metalloproteinase-9 activity. Connective Tissue Research, 44 Suppl 1, 79-84.
Yu X, Collin-Osdoby P, Osdoby P. SDF-1 Increases Recruitment of Osteoclast Precursors By Upregulation of Matrix Metalloproteinase-9 Activity. Connect Tissue Res. 2003;44 Suppl 1:79-84. PubMed PMID: 12952178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SDF-1 increases recruitment of osteoclast precursors by upregulation of matrix metalloproteinase-9 activity. AU - Yu,Xuefeng, AU - Collin-Osdoby,Patricia, AU - Osdoby,Philip, PY - 2003/9/4/pubmed PY - 2004/1/6/medline PY - 2003/9/4/entrez SP - 79 EP - 84 JF - Connective tissue research JO - Connect Tissue Res VL - 44 Suppl 1 N2 - Although chemokines play essential roles in the trafficking and homing of many circulating hematopoietic cell types, their potential influences on osteoclast (OC) recruitment or bone remodeling are not well known. Therefore, chemokine receptor expression was analyzed by RNase protection assay during OC formation induced by RANKL in a murine mononuclear cell line (RAW 264.7). Relatively high CXCR4 expression was detected in RAW cells (pre-OCs), whereas CXCR4 levels were downregulated during RAW-OC development. SDF-1, the unique ligand for CXCR4, stimulated RAW cell production of matrix metalloproteinase (MMP)-9 activity, a matrix-degrading enzyme essential for pre-OC migration into the developing bone marrow cavity. Induced MMP-9 activity in RAW cells was associated with their increased MMP-dependent transmigration through a collagen gel in response to SDF-1. We conclude that SDF-1 stimulation of MMP-9 activity in pre-OCs may be a key aspect of their recruitment to bone and migration within the marrow to sites for OC differentiation and bone resorption. SN - 0300-8207 UR - https://www.unboundmedicine.com/medline/citation/12952178/SDF_1_increases_recruitment_of_osteoclast_precursors_by_upregulation_of_matrix_metalloproteinase_9_activity_ L2 - https://medlineplus.gov/stemcells.html DB - PRIME DP - Unbound Medicine ER -