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Activating (P253R, C278F) and dominant negative mutations of FGFR2: differential effects on calvarial bone cell proliferation, differentiation, and mineralization.
Connect Tissue Res. 2003; 44 Suppl 1:292-7.CT

Abstract

Various activating mutations of FgfR2 have been linked to a number of craniosynostosis syndromes, suggesting that FGFR2-mediated signaling plays significant roles in intramembranous bone formation. To define (i) the roles of FGFR2-mediated signaling in osteogenesis and (ii) bone cell functions affected by abnormal signaling induced by craniosynostosis mutations, chicken calvarial osteoblasts were infected with replication competent avian sarcoma viruses expressing FgfR2 with dominant negative (DN), P253R (Apert), or C278F (Pfeiffer and Crouzon) mutation. Analyses of the infected osteoblasts revealed that attenuated FGF/FGFR signaling by DN-FgfR2 resulted in a decrease in cell proliferation and accelerated mineralization. In contrast, the C278F mutation, which causes ligand-independent activation of the receptor, significantly stimulated cell proliferation and inhibited mineralization. Interestingly, the P253R mutation, which does not cause ligand-independent activation of the receptor, showed a weaker mitogenic effect than the C278F mutation and did not inhibit mineralization. Gene expression analysis also revealed diverse effects of C278F and P253R mutations on expression of several osteogenic genes. Based on these results, we conclude that one of the major functions of FGFR2 is to mediate mitogenic signals in osteoblasts and that distinctively different cellular mechanisms underlie the pathogenesis of craniosynostosis phenotypes resulting from P253R and C278F mutations of the FGFR2 gene.

Authors+Show Affiliations

Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12952211

Citation

Ratisoontorn, Chootima, et al. "Activating (P253R, C278F) and Dominant Negative Mutations of FGFR2: Differential Effects On Calvarial Bone Cell Proliferation, Differentiation, and Mineralization." Connective Tissue Research, vol. 44 Suppl 1, 2003, pp. 292-7.
Ratisoontorn C, Fan GF, McEntee K, et al. Activating (P253R, C278F) and dominant negative mutations of FGFR2: differential effects on calvarial bone cell proliferation, differentiation, and mineralization. Connect Tissue Res. 2003;44 Suppl 1:292-7.
Ratisoontorn, C., Fan, G. F., McEntee, K., & Nah, H. D. (2003). Activating (P253R, C278F) and dominant negative mutations of FGFR2: differential effects on calvarial bone cell proliferation, differentiation, and mineralization. Connective Tissue Research, 44 Suppl 1, 292-7.
Ratisoontorn C, et al. Activating (P253R, C278F) and Dominant Negative Mutations of FGFR2: Differential Effects On Calvarial Bone Cell Proliferation, Differentiation, and Mineralization. Connect Tissue Res. 2003;44 Suppl 1:292-7. PubMed PMID: 12952211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activating (P253R, C278F) and dominant negative mutations of FGFR2: differential effects on calvarial bone cell proliferation, differentiation, and mineralization. AU - Ratisoontorn,Chootima, AU - Fan,Gao-Feng, AU - McEntee,Kerry, AU - Nah,Hyun-Duck, PY - 2003/9/4/pubmed PY - 2004/1/6/medline PY - 2003/9/4/entrez SP - 292 EP - 7 JF - Connective tissue research JO - Connect. Tissue Res. VL - 44 Suppl 1 N2 - Various activating mutations of FgfR2 have been linked to a number of craniosynostosis syndromes, suggesting that FGFR2-mediated signaling plays significant roles in intramembranous bone formation. To define (i) the roles of FGFR2-mediated signaling in osteogenesis and (ii) bone cell functions affected by abnormal signaling induced by craniosynostosis mutations, chicken calvarial osteoblasts were infected with replication competent avian sarcoma viruses expressing FgfR2 with dominant negative (DN), P253R (Apert), or C278F (Pfeiffer and Crouzon) mutation. Analyses of the infected osteoblasts revealed that attenuated FGF/FGFR signaling by DN-FgfR2 resulted in a decrease in cell proliferation and accelerated mineralization. In contrast, the C278F mutation, which causes ligand-independent activation of the receptor, significantly stimulated cell proliferation and inhibited mineralization. Interestingly, the P253R mutation, which does not cause ligand-independent activation of the receptor, showed a weaker mitogenic effect than the C278F mutation and did not inhibit mineralization. Gene expression analysis also revealed diverse effects of C278F and P253R mutations on expression of several osteogenic genes. Based on these results, we conclude that one of the major functions of FGFR2 is to mediate mitogenic signals in osteoblasts and that distinctively different cellular mechanisms underlie the pathogenesis of craniosynostosis phenotypes resulting from P253R and C278F mutations of the FGFR2 gene. SN - 0300-8207 UR - https://www.unboundmedicine.com/medline/citation/12952211/Activating__P253R_C278F__and_dominant_negative_mutations_of_FGFR2:_differential_effects_on_calvarial_bone_cell_proliferation_differentiation_and_mineralization_ L2 - https://www.lens.org/lens/search?q=citation_id:12952211 DB - PRIME DP - Unbound Medicine ER -