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Determinants of fetal leptin synthesis, fat mass, and circulating leptin concentrations in well-nourished ewes in late pregnancy.
Endocrinology 2003; 144(11):4947-54E

Abstract

We have investigated the factors regulating leptin synthesis, fat deposition, and circulating leptin concentrations in fetuses of well nourished ewes in late pregnancy. Vascular catheters were surgically inserted in 17 pregnant ewes and their fetuses at 103-120 d gestation (term = 147 +/- 3 d). Ewes were fed a diet providing either 100% (control; n = 9) or approximately 155% (well fed; n = 8) of the maintenance energy requirements and fetal perirenal and interscapular fat depots were collected at 139-141 d gestation. There was a significant relationship between the relative mass of fetal unilocular fat and fetal glucose (relative mass of unilocular fat, 1.14; fetal glucose, +0.16; r = 0.50; P < 0.04; n = 17), but not insulin, concentrations in the control and well-fed groups. In contrast to the controls, there was a positive relationship between the relative abundance of leptin mRNA and fetal insulin, but not glucose, concentrations in fetal perirenal adipose tissue in the well-fed group. A moderate increase in maternal nutrition also resulted in a strong reciprocal relationship between uncoupling protein 1 and leptin expression in fetal perirenal adipose tissue in late gestation (well-fed group: uncoupling protein 1 mRNA:18S rRNA, -0.51; leptin mRNA:beta-actin mRNA, +1.53; r = 0.80; P < 0.02; n = 8). These studies provide evidence that fetal glucose and insulin differentially regulate fetal fat deposition and leptin mRNA expression within the fetal perirenal fat depot in the well nourished animal during late gestation.

Authors+Show Affiliations

Department of Physiology, University of Adelaide, South Australia 5000, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12959967

Citation

Mühlhäusler, B S., et al. "Determinants of Fetal Leptin Synthesis, Fat Mass, and Circulating Leptin Concentrations in Well-nourished Ewes in Late Pregnancy." Endocrinology, vol. 144, no. 11, 2003, pp. 4947-54.
Mühlhäusler BS, Roberts CT, Yuen BS, et al. Determinants of fetal leptin synthesis, fat mass, and circulating leptin concentrations in well-nourished ewes in late pregnancy. Endocrinology. 2003;144(11):4947-54.
Mühlhäusler, B. S., Roberts, C. T., Yuen, B. S., Marrocco, E., Budge, H., Symonds, M. E., ... McMillen, I. C. (2003). Determinants of fetal leptin synthesis, fat mass, and circulating leptin concentrations in well-nourished ewes in late pregnancy. Endocrinology, 144(11), pp. 4947-54.
Mühlhäusler BS, et al. Determinants of Fetal Leptin Synthesis, Fat Mass, and Circulating Leptin Concentrations in Well-nourished Ewes in Late Pregnancy. Endocrinology. 2003;144(11):4947-54. PubMed PMID: 12959967.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determinants of fetal leptin synthesis, fat mass, and circulating leptin concentrations in well-nourished ewes in late pregnancy. AU - Mühlhäusler,B S, AU - Roberts,C T, AU - Yuen,B S J, AU - Marrocco,E, AU - Budge,H, AU - Symonds,M E, AU - McFarlane,J R, AU - Kauter,K G, AU - Stagg,P, AU - Pearse,J K, AU - McMillen,I C, Y1 - 2003/07/24/ PY - 2003/9/10/pubmed PY - 2003/12/4/medline PY - 2003/9/10/entrez SP - 4947 EP - 54 JF - Endocrinology JO - Endocrinology VL - 144 IS - 11 N2 - We have investigated the factors regulating leptin synthesis, fat deposition, and circulating leptin concentrations in fetuses of well nourished ewes in late pregnancy. Vascular catheters were surgically inserted in 17 pregnant ewes and their fetuses at 103-120 d gestation (term = 147 +/- 3 d). Ewes were fed a diet providing either 100% (control; n = 9) or approximately 155% (well fed; n = 8) of the maintenance energy requirements and fetal perirenal and interscapular fat depots were collected at 139-141 d gestation. There was a significant relationship between the relative mass of fetal unilocular fat and fetal glucose (relative mass of unilocular fat, 1.14; fetal glucose, +0.16; r = 0.50; P < 0.04; n = 17), but not insulin, concentrations in the control and well-fed groups. In contrast to the controls, there was a positive relationship between the relative abundance of leptin mRNA and fetal insulin, but not glucose, concentrations in fetal perirenal adipose tissue in the well-fed group. A moderate increase in maternal nutrition also resulted in a strong reciprocal relationship between uncoupling protein 1 and leptin expression in fetal perirenal adipose tissue in late gestation (well-fed group: uncoupling protein 1 mRNA:18S rRNA, -0.51; leptin mRNA:beta-actin mRNA, +1.53; r = 0.80; P < 0.02; n = 8). These studies provide evidence that fetal glucose and insulin differentially regulate fetal fat deposition and leptin mRNA expression within the fetal perirenal fat depot in the well nourished animal during late gestation. SN - 0013-7227 UR - https://www.unboundmedicine.com/medline/citation/12959967/Determinants_of_fetal_leptin_synthesis_fat_mass_and_circulating_leptin_concentrations_in_well_nourished_ewes_in_late_pregnancy_ L2 - https://academic.oup.com/endo/article-lookup/doi/10.1210/en.2003-0555 DB - PRIME DP - Unbound Medicine ER -