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Multiplex RT-PCR for the detection of common BCR-ABL fusion transcripts in paraffin-embedded tissues from patients with chronic myeloid leukemia and acute lymphoblastic leukemia.
Diagn Mol Pathol 2003; 12(3):119-23DM

Abstract

Diagnosis of chronic myeloid leukemia and acute lymphoblastic leukemia requires the investigation of the Philadelphia chromosome translocation t(9;22) or the molecular detection of BCR-ABL fusion transcripts. Determination of the type of fusion transcript is crucial for quantitative molecular monitoring the course of the disease during treatment. Histopathologists, who usually use formalin-fixed tissues, may be confronted with the need to investigate the BCR-ABL rearrangement when evaluating tumor forming infiltrates and bone marrow trephines from patients presenting with chronic myeloproliferative disorders. Therefore, we have established a one-tube multiplex RT-PCR for the detection of common BCR-ABL fusion transcripts (b2a2, b3a2, e1a2) in routinely processed tissues and bone marrow trephines with respect to the inevitable fragmentation of ribonucleic acids in these specimens. RT-PCR products allow distinct and unequivocal differentiation of the underlying fusion in either the Major- or minor-breakpoint cluster region. Detection of BCR-ABL fusion transcripts by multiplex RT-PCR in routinely processed and fixed tissues is a time- and cost-sparing tool for definite diagnosis of typical chronic myeloid leukemia and Philadelphia chromosome positive acute lymphoblastic leukemia.

Authors+Show Affiliations

Institute of Pathology, Medizinische Hochschule Hanover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. Bock.Oliver@MH-Hannover.de

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12960692

Citation

Bock, Oliver, et al. "Multiplex RT-PCR for the Detection of Common BCR-ABL Fusion Transcripts in Paraffin-embedded Tissues From Patients With Chronic Myeloid Leukemia and Acute Lymphoblastic Leukemia." Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B, vol. 12, no. 3, 2003, pp. 119-23.
Bock O, Reising D, Kreipe H. Multiplex RT-PCR for the detection of common BCR-ABL fusion transcripts in paraffin-embedded tissues from patients with chronic myeloid leukemia and acute lymphoblastic leukemia. Diagn Mol Pathol. 2003;12(3):119-23.
Bock, O., Reising, D., & Kreipe, H. (2003). Multiplex RT-PCR for the detection of common BCR-ABL fusion transcripts in paraffin-embedded tissues from patients with chronic myeloid leukemia and acute lymphoblastic leukemia. Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B, 12(3), pp. 119-23.
Bock O, Reising D, Kreipe H. Multiplex RT-PCR for the Detection of Common BCR-ABL Fusion Transcripts in Paraffin-embedded Tissues From Patients With Chronic Myeloid Leukemia and Acute Lymphoblastic Leukemia. Diagn Mol Pathol. 2003;12(3):119-23. PubMed PMID: 12960692.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiplex RT-PCR for the detection of common BCR-ABL fusion transcripts in paraffin-embedded tissues from patients with chronic myeloid leukemia and acute lymphoblastic leukemia. AU - Bock,Oliver, AU - Reising,Dunja, AU - Kreipe,Hans, PY - 2003/9/10/pubmed PY - 2004/3/31/medline PY - 2003/9/10/entrez SP - 119 EP - 23 JF - Diagnostic molecular pathology : the American journal of surgical pathology, part B JO - Diagn. Mol. Pathol. VL - 12 IS - 3 N2 - Diagnosis of chronic myeloid leukemia and acute lymphoblastic leukemia requires the investigation of the Philadelphia chromosome translocation t(9;22) or the molecular detection of BCR-ABL fusion transcripts. Determination of the type of fusion transcript is crucial for quantitative molecular monitoring the course of the disease during treatment. Histopathologists, who usually use formalin-fixed tissues, may be confronted with the need to investigate the BCR-ABL rearrangement when evaluating tumor forming infiltrates and bone marrow trephines from patients presenting with chronic myeloproliferative disorders. Therefore, we have established a one-tube multiplex RT-PCR for the detection of common BCR-ABL fusion transcripts (b2a2, b3a2, e1a2) in routinely processed tissues and bone marrow trephines with respect to the inevitable fragmentation of ribonucleic acids in these specimens. RT-PCR products allow distinct and unequivocal differentiation of the underlying fusion in either the Major- or minor-breakpoint cluster region. Detection of BCR-ABL fusion transcripts by multiplex RT-PCR in routinely processed and fixed tissues is a time- and cost-sparing tool for definite diagnosis of typical chronic myeloid leukemia and Philadelphia chromosome positive acute lymphoblastic leukemia. SN - 1052-9551 UR - https://www.unboundmedicine.com/medline/citation/12960692/Multiplex_RT_PCR_for_the_detection_of_common_BCR_ABL_fusion_transcripts_in_paraffin_embedded_tissues_from_patients_with_chronic_myeloid_leukemia_and_acute_lymphoblastic_leukemia_ L2 - http://Insights.ovid.com/pubmed?pmid=12960692 DB - PRIME DP - Unbound Medicine ER -