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Phenotypic analysis of plasma cells in bone marrow using flow cytometry in AL amyloidosis.
Amyloid. 2003 Jun; 10(2):110-6.A

Abstract

AL amyloidosis is an intractable disease resulting from a plasma cell dyscrasia which has a wide clinical spectrum. To investigate the phenotype of plasma cells in the bone marrow, a flow cytometric analysis was carried out in 10 patients with this disease (mean age, 57.8 +/- 7.9 years) and controls with M-protein (positive controls, n = 4) and without it (negative controls, n = 8). All patients were shown to have either A kappa- or A lambda-immunoreactive amyloid deposits on the biopsied tissues. On flow cytometry CD38++CD19+CD56- cells (polyclonal plasma cells) showed no significant difference between patients (0.59 +/- 0.37%) and either negative (2.25 +/- 2.84%) or positive controls (0.38 +/- 0.20%), while CD38++CD19-CD56+ cells (monoclonal plasma cells) showed a significantly higher level in the patients (1.34 +/- 1.54%) than in either negative (0.041 +/- 0.004%, p < 0.005) or positive controls (0.11 +/- 0.09%, p < 0.05). With respect to maturation of plasma cells, five of the patients (50%), three of the positive controls (75%) and all of the negative controls showed a dominant proliferation of mature subtype (CD45+MPC-1+CD49e- or CD45+MPC-1+CD49e+). Immature (CD45+MPC-1- or CD45-MPC-1-) and intermediate (CD45-MPC-1+CD49e-) subtypes were dominantly present in the bone marrow in 2 and 3 patients, respectively. In AL amyloidosis monoclonal plasma cells producing M-protein can be easily and reliably detected in the bone marrow by flow cytometry. This analysis might provide plasma cell phenotypic markers useful for assessing the prognosis and for monitoring the response to treatment.

Authors+Show Affiliations

Third Department of Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan. matsuda@hsp.md.shinshu-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12964418

Citation

Matsuda, Masayuki, et al. "Phenotypic Analysis of Plasma Cells in Bone Marrow Using Flow Cytometry in AL Amyloidosis." Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis, vol. 10, no. 2, 2003, pp. 110-6.
Matsuda M, Gono T, Shimojima Y, et al. Phenotypic analysis of plasma cells in bone marrow using flow cytometry in AL amyloidosis. Amyloid. 2003;10(2):110-6.
Matsuda, M., Gono, T., Shimojima, Y., Hoshii, Y., & Ikeda, S. (2003). Phenotypic analysis of plasma cells in bone marrow using flow cytometry in AL amyloidosis. Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis, 10(2), 110-6.
Matsuda M, et al. Phenotypic Analysis of Plasma Cells in Bone Marrow Using Flow Cytometry in AL Amyloidosis. Amyloid. 2003;10(2):110-6. PubMed PMID: 12964418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic analysis of plasma cells in bone marrow using flow cytometry in AL amyloidosis. AU - Matsuda,Masayuki, AU - Gono,Takahisa, AU - Shimojima,Yasuhiro, AU - Hoshii,Yoshinobu, AU - Ikeda,Shu-ichi, PY - 2003/9/11/pubmed PY - 2004/4/20/medline PY - 2003/9/11/entrez SP - 110 EP - 6 JF - Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis JO - Amyloid VL - 10 IS - 2 N2 - AL amyloidosis is an intractable disease resulting from a plasma cell dyscrasia which has a wide clinical spectrum. To investigate the phenotype of plasma cells in the bone marrow, a flow cytometric analysis was carried out in 10 patients with this disease (mean age, 57.8 +/- 7.9 years) and controls with M-protein (positive controls, n = 4) and without it (negative controls, n = 8). All patients were shown to have either A kappa- or A lambda-immunoreactive amyloid deposits on the biopsied tissues. On flow cytometry CD38++CD19+CD56- cells (polyclonal plasma cells) showed no significant difference between patients (0.59 +/- 0.37%) and either negative (2.25 +/- 2.84%) or positive controls (0.38 +/- 0.20%), while CD38++CD19-CD56+ cells (monoclonal plasma cells) showed a significantly higher level in the patients (1.34 +/- 1.54%) than in either negative (0.041 +/- 0.004%, p < 0.005) or positive controls (0.11 +/- 0.09%, p < 0.05). With respect to maturation of plasma cells, five of the patients (50%), three of the positive controls (75%) and all of the negative controls showed a dominant proliferation of mature subtype (CD45+MPC-1+CD49e- or CD45+MPC-1+CD49e+). Immature (CD45+MPC-1- or CD45-MPC-1-) and intermediate (CD45-MPC-1+CD49e-) subtypes were dominantly present in the bone marrow in 2 and 3 patients, respectively. In AL amyloidosis monoclonal plasma cells producing M-protein can be easily and reliably detected in the bone marrow by flow cytometry. This analysis might provide plasma cell phenotypic markers useful for assessing the prognosis and for monitoring the response to treatment. SN - 1350-6129 UR - https://www.unboundmedicine.com/medline/citation/12964418/Phenotypic_analysis_of_plasma_cells_in_bone_marrow_using_flow_cytometry_in_AL_amyloidosis_ L2 - http://www.tandfonline.com/doi/full/10.3109/13506120309041732 DB - PRIME DP - Unbound Medicine ER -