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[Identification of the causative agents of glanders and melioidosis by polymerase chain reaction].

Abstract

Burkholderia mallei and B. pseudomallei are causative agents of glanders and melioidosis, respectively, i.e. severe and fatal infection diseases of man and animal. The computer-based analysis of the 23S rRNA gene sites was used for selecting the primers. Two pairs of primers were chosen for the identification of B. mallei and Bpseudomallei. DNAs from 48 B. pseudomallei and 15 strains of B. mallei, unlike from other geterological bacteria, were positively amplified. Therefore, the method of polymerase chain reaction can be used in laboratory diagnosis of glanders and melioidosis.

Authors+Show Affiliations

Research Institute for Plague Control, Volgograd.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

12966921

Citation

Tkachenko, G A., et al. "[Identification of the Causative Agents of Glanders and Melioidosis By Polymerase Chain Reaction]." Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, 2003, pp. 18-22.
Tkachenko GA, Antonov VA, Zamaraev VS, et al. [Identification of the causative agents of glanders and melioidosis by polymerase chain reaction]. Mol Gen Mikrobiol Virusol. 2003.
Tkachenko, G. A., Antonov, V. A., Zamaraev, V. S., & Iliukhin, V. I. (2003). [Identification of the causative agents of glanders and melioidosis by polymerase chain reaction]. Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, (3), 18-22.
Tkachenko GA, et al. [Identification of the Causative Agents of Glanders and Melioidosis By Polymerase Chain Reaction]. Mol Gen Mikrobiol Virusol. 2003;(3)18-22. PubMed PMID: 12966921.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Identification of the causative agents of glanders and melioidosis by polymerase chain reaction]. AU - Tkachenko,G A, AU - Antonov,V A, AU - Zamaraev,V S, AU - Iliukhin,V I, PY - 2003/9/12/pubmed PY - 2003/10/28/medline PY - 2003/9/12/entrez SP - 18 EP - 22 JF - Molekuliarnaia genetika, mikrobiologiia i virusologiia JO - Mol Gen Mikrobiol Virusol IS - 3 N2 - Burkholderia mallei and B. pseudomallei are causative agents of glanders and melioidosis, respectively, i.e. severe and fatal infection diseases of man and animal. The computer-based analysis of the 23S rRNA gene sites was used for selecting the primers. Two pairs of primers were chosen for the identification of B. mallei and Bpseudomallei. DNAs from 48 B. pseudomallei and 15 strains of B. mallei, unlike from other geterological bacteria, were positively amplified. Therefore, the method of polymerase chain reaction can be used in laboratory diagnosis of glanders and melioidosis. SN - 0208-0613 UR - https://www.unboundmedicine.com/medline/citation/12966921/[Identification_of_the_causative_agents_of_glanders_and_melioidosis_by_polymerase_chain_reaction]_ DB - PRIME DP - Unbound Medicine ER -