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    Aged garlic extract attenuates gentamicin induced renal damage and oxidative stress in rats.

    Abstract

    Gentamicin (GM) is an antibiotic whose clinical use is limited by its nephrotoxicity. Experimental evidences suggest a role of reactive oxygen species in GM-induced nephrotoxicity. Therefore, we investigated if aged garlic extract (AGE), an antioxidant, has a protective role in this experimental model. Four groups of male Wistar rats were studied: 1) Control (CT), injected subcutaneously (s.c.) and intraperitoneally (i.p.) with saline, 2) GM, treated s.c. with GM (70 mg/kg/12 hours/4 days), 3) AGE, treated i.p with AGE (1.2 mL/kg/12 hours/6 days), and 4) GM + AGE treated with GM and AGE. The treatment with AGE started two days before the first dose of GM (GM + AGE group) or saline (AGE group). Animals were sacrificed on day 5, and blood, urine, and kidneys were obtained. Nephrotoxicity was made evident by: 1) the increase in blood urea nitrogen and plasma creatinine, 2) the decrease in plasma glutathione peroxidase (GPx) activity and the urinary increase in N-acetyl-beta-D-glucosaminidase activity and total protein, and 3) necrosis of proximal tubular cells. These alterations were prevented or ameliorated by AGE treatment. Furthermore, AGE prevented the GM-induced increase in the renal levels of oxidative stress markers: nitrotyrosine and protein carbonyl groups and the decrease in manganese superoxide dismutase (Mn-SOD), GPx, and glutathione reductase (GR) activities. The protective effect of AGE was associated with the decrease in the oxidative stress and the preservation of Mn-SOD, GPx, and GR activities in renal cortex. These data suggest that AGE may be a useful agent for the prevention of GM-nephrotoxicity.

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    Authors+Show Affiliations

    Maldonado PD
    ,

    Department of Biology, Faculty of Chemistry, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 D.F., México, Mexico.

    Barrera D
    ,
    Medina-Campos ON
    ,
    Hernández-Pando R
    ,
    Ibarra-Rubio ME
    ,
    Pedraza-Chaverrí J

    Source

    Life sciences 73:20 2003 Oct 03 pg 2543-56

    MeSH

    Animals
    Anti-Bacterial Agents
    Blotting, Western
    Body Weight
    Catalase
    Free Radical Scavengers
    Garlic
    Gentamicins
    Indicators and Reagents
    Kidney
    Kidney Cortex
    Kidney Diseases
    Kidney Glomerulus
    Kidney Tubules
    Male
    Oxidative Stress
    Plant Extracts
    Rats
    Rats, Wistar
    Superoxides
    Urination

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    12967679

    Citation

    TY - JOUR T1 - Aged garlic extract attenuates gentamicin induced renal damage and oxidative stress in rats. AU - Maldonado,Perla D, AU - Barrera,Diana, AU - Medina-Campos,Omar N, AU - Hernández-Pando,Rogelio, AU - Ibarra-Rubio,María E, AU - Pedraza-Chaverrí,José, PY - 2003/9/12/pubmed PY - 2003/10/10/medline PY - 2003/9/12/entrez SP - 2543 EP - 56 JF - Life sciences JO - Life Sci. VL - 73 IS - 20 N2 - Gentamicin (GM) is an antibiotic whose clinical use is limited by its nephrotoxicity. Experimental evidences suggest a role of reactive oxygen species in GM-induced nephrotoxicity. Therefore, we investigated if aged garlic extract (AGE), an antioxidant, has a protective role in this experimental model. Four groups of male Wistar rats were studied: 1) Control (CT), injected subcutaneously (s.c.) and intraperitoneally (i.p.) with saline, 2) GM, treated s.c. with GM (70 mg/kg/12 hours/4 days), 3) AGE, treated i.p with AGE (1.2 mL/kg/12 hours/6 days), and 4) GM + AGE treated with GM and AGE. The treatment with AGE started two days before the first dose of GM (GM + AGE group) or saline (AGE group). Animals were sacrificed on day 5, and blood, urine, and kidneys were obtained. Nephrotoxicity was made evident by: 1) the increase in blood urea nitrogen and plasma creatinine, 2) the decrease in plasma glutathione peroxidase (GPx) activity and the urinary increase in N-acetyl-beta-D-glucosaminidase activity and total protein, and 3) necrosis of proximal tubular cells. These alterations were prevented or ameliorated by AGE treatment. Furthermore, AGE prevented the GM-induced increase in the renal levels of oxidative stress markers: nitrotyrosine and protein carbonyl groups and the decrease in manganese superoxide dismutase (Mn-SOD), GPx, and glutathione reductase (GR) activities. The protective effect of AGE was associated with the decrease in the oxidative stress and the preservation of Mn-SOD, GPx, and GR activities in renal cortex. These data suggest that AGE may be a useful agent for the prevention of GM-nephrotoxicity. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/12967679/Aged_garlic_extract_attenuates_gentamicin_induced_renal_damage_and_oxidative_stress_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S002432050300609X ER -