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Aged garlic extract attenuates gentamicin induced renal damage and oxidative stress in rats.
Life Sci 2003; 73(20):2543-56LS

Abstract

Gentamicin (GM) is an antibiotic whose clinical use is limited by its nephrotoxicity. Experimental evidences suggest a role of reactive oxygen species in GM-induced nephrotoxicity. Therefore, we investigated if aged garlic extract (AGE), an antioxidant, has a protective role in this experimental model. Four groups of male Wistar rats were studied: 1) Control (CT), injected subcutaneously (s.c.) and intraperitoneally (i.p.) with saline, 2) GM, treated s.c. with GM (70 mg/kg/12 hours/4 days), 3) AGE, treated i.p with AGE (1.2 mL/kg/12 hours/6 days), and 4) GM + AGE treated with GM and AGE. The treatment with AGE started two days before the first dose of GM (GM + AGE group) or saline (AGE group). Animals were sacrificed on day 5, and blood, urine, and kidneys were obtained. Nephrotoxicity was made evident by: 1) the increase in blood urea nitrogen and plasma creatinine, 2) the decrease in plasma glutathione peroxidase (GPx) activity and the urinary increase in N-acetyl-beta-D-glucosaminidase activity and total protein, and 3) necrosis of proximal tubular cells. These alterations were prevented or ameliorated by AGE treatment. Furthermore, AGE prevented the GM-induced increase in the renal levels of oxidative stress markers: nitrotyrosine and protein carbonyl groups and the decrease in manganese superoxide dismutase (Mn-SOD), GPx, and glutathione reductase (GR) activities. The protective effect of AGE was associated with the decrease in the oxidative stress and the preservation of Mn-SOD, GPx, and GR activities in renal cortex. These data suggest that AGE may be a useful agent for the prevention of GM-nephrotoxicity.

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    Authors+Show Affiliations

    Maldonado PD
    Department of Biology, Faculty of Chemistry, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 D.F., México, Mexico.
    Barrera D
    No affiliation info available
    Medina-Campos ON
    No affiliation info available
    Hernández-Pando R
    No affiliation info available
    Ibarra-Rubio ME
    No affiliation info available
    Pedraza-Chaverrí J
    No affiliation info available

    MeSH

    AnimalsAnti-Bacterial AgentsBlotting, WesternBody WeightCatalaseFree Radical ScavengersGarlicGentamicinsIndicators and ReagentsKidneyKidney CortexKidney DiseasesKidney GlomerulusKidney TubulesMaleOxidative StressPlant ExtractsRatsRats, WistarSuperoxidesUrination

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    12967679

    Citation

    Maldonado, Perla D., et al. "Aged Garlic Extract Attenuates Gentamicin Induced Renal Damage and Oxidative Stress in Rats." Life Sciences, vol. 73, no. 20, 2003, pp. 2543-56.
    Maldonado PD, Barrera D, Medina-Campos ON, et al. Aged garlic extract attenuates gentamicin induced renal damage and oxidative stress in rats. Life Sci. 2003;73(20):2543-56.
    Maldonado, P. D., Barrera, D., Medina-Campos, O. N., Hernández-Pando, R., Ibarra-Rubio, M. E., & Pedraza-Chaverrí, J. (2003). Aged garlic extract attenuates gentamicin induced renal damage and oxidative stress in rats. Life Sciences, 73(20), pp. 2543-56.
    Maldonado PD, et al. Aged Garlic Extract Attenuates Gentamicin Induced Renal Damage and Oxidative Stress in Rats. Life Sci. 2003 Oct 3;73(20):2543-56. PubMed PMID: 12967679.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Aged garlic extract attenuates gentamicin induced renal damage and oxidative stress in rats. AU - Maldonado,Perla D, AU - Barrera,Diana, AU - Medina-Campos,Omar N, AU - Hernández-Pando,Rogelio, AU - Ibarra-Rubio,María E, AU - Pedraza-Chaverrí,José, PY - 2003/9/12/pubmed PY - 2003/10/10/medline PY - 2003/9/12/entrez SP - 2543 EP - 56 JF - Life sciences JO - Life Sci. VL - 73 IS - 20 N2 - Gentamicin (GM) is an antibiotic whose clinical use is limited by its nephrotoxicity. Experimental evidences suggest a role of reactive oxygen species in GM-induced nephrotoxicity. Therefore, we investigated if aged garlic extract (AGE), an antioxidant, has a protective role in this experimental model. Four groups of male Wistar rats were studied: 1) Control (CT), injected subcutaneously (s.c.) and intraperitoneally (i.p.) with saline, 2) GM, treated s.c. with GM (70 mg/kg/12 hours/4 days), 3) AGE, treated i.p with AGE (1.2 mL/kg/12 hours/6 days), and 4) GM + AGE treated with GM and AGE. The treatment with AGE started two days before the first dose of GM (GM + AGE group) or saline (AGE group). Animals were sacrificed on day 5, and blood, urine, and kidneys were obtained. Nephrotoxicity was made evident by: 1) the increase in blood urea nitrogen and plasma creatinine, 2) the decrease in plasma glutathione peroxidase (GPx) activity and the urinary increase in N-acetyl-beta-D-glucosaminidase activity and total protein, and 3) necrosis of proximal tubular cells. These alterations were prevented or ameliorated by AGE treatment. Furthermore, AGE prevented the GM-induced increase in the renal levels of oxidative stress markers: nitrotyrosine and protein carbonyl groups and the decrease in manganese superoxide dismutase (Mn-SOD), GPx, and glutathione reductase (GR) activities. The protective effect of AGE was associated with the decrease in the oxidative stress and the preservation of Mn-SOD, GPx, and GR activities in renal cortex. These data suggest that AGE may be a useful agent for the prevention of GM-nephrotoxicity. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/12967679/Aged_garlic_extract_attenuates_gentamicin_induced_renal_damage_and_oxidative_stress_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S002432050300609X DB - PRIME DP - Unbound Medicine ER -