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Construction of shuttle vectors for cloning in Escherichia coli and Acetobacter pasteurianus.
Folia Microbiol (Praha). 1992; 37(6):395-400.FM

Abstract

New cloning vectors were prepared with the aid of a large plasmid isolated from Acetobacter pasteurianus and from plasmids pBR322 and pUC4-KAPA. Of the prepared cloning vectors, pACK5 contains a gene coding for kanamycin resistance, pACT7 and pACT71 contain a gene coding for tetracycline resistance and vector pACG3 with a gene coding for both kanamycin and tetracycline resistance. The vectors prepared only contained the beginning of replication from the pAC1 plasmid and possessed the ability to replicate within E. coli and A. pasteurianus. The vectors are highly stable in both strains and during the 5-d cultivation under nonselective conditions are not eliminated.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Science, Comenius University, Bratislava, Czechoslovakia.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1296922

Citation

Grones, J, and J Turna. "Construction of Shuttle Vectors for Cloning in Escherichia Coli and Acetobacter Pasteurianus." Folia Microbiologica, vol. 37, no. 6, 1992, pp. 395-400.
Grones J, Turna J. Construction of shuttle vectors for cloning in Escherichia coli and Acetobacter pasteurianus. Folia Microbiol (Praha). 1992;37(6):395-400.
Grones, J., & Turna, J. (1992). Construction of shuttle vectors for cloning in Escherichia coli and Acetobacter pasteurianus. Folia Microbiologica, 37(6), 395-400.
Grones J, Turna J. Construction of Shuttle Vectors for Cloning in Escherichia Coli and Acetobacter Pasteurianus. Folia Microbiol (Praha). 1992;37(6):395-400. PubMed PMID: 1296922.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction of shuttle vectors for cloning in Escherichia coli and Acetobacter pasteurianus. AU - Grones,J, AU - Turna,J, PY - 1992/1/1/pubmed PY - 1992/1/1/medline PY - 1992/1/1/entrez SP - 395 EP - 400 JF - Folia microbiologica JO - Folia Microbiol (Praha) VL - 37 IS - 6 N2 - New cloning vectors were prepared with the aid of a large plasmid isolated from Acetobacter pasteurianus and from plasmids pBR322 and pUC4-KAPA. Of the prepared cloning vectors, pACK5 contains a gene coding for kanamycin resistance, pACT7 and pACT71 contain a gene coding for tetracycline resistance and vector pACG3 with a gene coding for both kanamycin and tetracycline resistance. The vectors prepared only contained the beginning of replication from the pAC1 plasmid and possessed the ability to replicate within E. coli and A. pasteurianus. The vectors are highly stable in both strains and during the 5-d cultivation under nonselective conditions are not eliminated. SN - 0015-5632 UR - https://www.unboundmedicine.com/medline/citation/1296922/Construction_of_shuttle_vectors_for_cloning_in_Escherichia_coli_and_Acetobacter_pasteurianus_ DB - PRIME DP - Unbound Medicine ER -